Immunomodulatory effect of bone marrow mesenchymal stem cells on T lymphocytes in patients with decompensated liver cirrhosis

Abstract

ABSTRACT. We explored the immunomodulatory effects of bone marrow mesenchymal stem cells (BMSCs) on peripheral blood T lymphocytes in patients with decompensation stage, hepatitis B-associated cirrhosis. MSCs from nine patients were analyzed by flow cytometry. Peripheral blood lymphocytes were isolated for fluorescent staining. Following stimulation by phytohemagglutinin (PHA), peripheral blood lymphocytes were co-cultured with BMSCs in serum and divided into four groups: (1) BMSC + lymphocyte + PHA contact culture group; (2) BMSC + lymphocyte + PHA non-contact culture group; (3) lymphocyte + PHA positive control group; and (4) lymphocyte-only negative control group. Lymphocyte proliferation and frequencies of CD4 + CD25 +

Cytotoxicity and apoptogenic properties of the standardized extract of Portulaca oleracea on glioblastoma multiforme cancer cell line (U-87): a mechanistic study

“…The live cells were counted using trypan blue solution % 0.04. The cell viability and the cell numbers were more than 98 % and 2.5×10 6 cells/mL, respectively in all samples (Guo et al, 2015[ 26 ], Askari et al, 2016[ 10 ]). The proliferation of isolated lymphocytes was assessed using colorimetric WST-1 assay kit (Roche Diagnostic, Mannheim, Germany) according to manufacturer's instruction.…”

“…Fifteen ml of brachial vein whole blood were prepared from each subject and collected into a heparinized tube. Peripheral blood mononuclear cells (PBMC) were separated using Ficoll density gradient as described previously (Boskabady et al, 2011[ 19 ]; Guo et al, 2015[ 26 ]; Askari et al; 2018[ 8 ][ 9 ]). The live cells were counted using trypan blue solution % 0.04.…”

Progress of Interference of Traditional Chinese Medicine on Cirrhosis Treated with Bone Marrow Mesenchymal Stem Cells

“…BMSCs secrete various soluble mediators, inhibit the proliferation and activation of various immune cells, and ultimately inhibit the activation of hepatic stellate cells. Research by Guo et al found that MSCs transplantation in patients with liver cirrhosis can inhibit the proliferation of peripheral blood T lymphocytes, upregulate the expression of CD4, CD25, and CD127, regulatory T cells (Treg), and also alleviate the Treg/Th17 imbalance [ 16 ]. Liu et al [ 17 ] also found that, during liver fibrosis, CD4+, CD25+, FoxD3, and other regulatory T cells in the liver increased, while NK cells decreased.…”

BMSC-derived Exosomes Protect Against Delayed Encephalopathy after Acute Carbon Monoxide Poisoning in Rats via Blockade of Notch Signaling

“…BMSC-derived exosomes are nano-sized extracellular vesicles loaded with various RNAs and proteins, and act as paracrine factors in intercellular communication[8] [9]. Exosome-based therapy is an emerging modality in various in ammatory conditions involving an overactive immune system [10] [11], and has shown promising effects in clinical trials and experimental models [12]. Several studies have demonstrated that the modulation of regulatory T cells (Tregs) is due to immunoregulatory effects of exosomes [13] [14].…”