Immunomodulatory assessment of Portulaca oleracea L. extract in a mouse model of colitis

Alfwuaires, Manal; Algefare, Abdulmohsen I.; Afkar, Eman; Salam, Sherine Abdel; El-Moaty, Heba Ibrahim Abd; Badr, Gehan M.

doi:10.1016/j.biopha.2021.112148

Cited by 15 publications

(4 citation statements)

References 56 publications

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“…Phenolic acids can inhibit NF-κB, COX-2, iNOS and PGE2, which demonstrate suppression of intestinal edema and amelioration of ulcerative colitis. Phenolic compounds can inhibit NO and PGE2, which results in the suppression of edema [36,[45][46][47][48].…”

Section: Discussionmentioning

confidence: 99%

Sinapic Acid Ameliorates Acetic Acid-Induced Ulcerative Colitis in Rats by Suppressing Inflammation, Oxidative Stress, and Apoptosis

et al. 2022

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Background: Ulcerative colitis (UC) is a long-term condition which results in inflammation and ulcers of the colon and rectum. The key indications of active disease are abdominal pain and diarrhea mixed with blood. Aims: We explore the underlying colon protective mechanism of sinapic acid (SA) against acetic acid (AA) induced ulcerative colitis in rats. The implications of inflammation, oxidative stress, and apoptosis are studied. Methodology: Twenty-four rats were distributed into four categories, normal control (NC), ulcerative colitis (UC), ulcerative Colitis with SA 40 mg/kg (SA 40 mg/kg + AA), and ulcerative colitis with prednisolone (PRDL 10 mg/kg + AA), and were pretreated orally with saline, saline and SA (40 mg/kg/day) or PRDL (10 mg/kg/day) respectively, for 7 days. UC was prompted by trans-rectal administration of 4% AA on the 5th day, colon tissues were surgically removed for gross morphology and histological inspection, oxidative stress, and inflammatory markers and immunoblot analysis of Bax, caspase-3, and Bcl-2. Results: Macroscopic and histological inspection demonstrated that both SA 40 mg/kg and PRDL (10 mg/kg/day) significantly ameliorates colonic injuries. In addition, both pretreatments significantly ameliorates AA-induced UC, oxidative stress, as indicated by suppressed malondialdehyde (MDA), nitric oxide (NO) levels and restoring antioxidant/oxidant balance as indicated by catalase and glutathione levels, suppressed inflammation via inhibiting cytokines TNF-α, IL-6, inflammatory markers MPO, PGE2, COX-2 and NF-κB and inhibiting the protein expression of Bax and caspase-3 apoptotic protein and increasing the anti-apoptotic protein, Bcl-2 thereby inhibiting apoptosis. Conclusion: Sinapic acid significantly ameliorates AA induced UC in rats by suppressing inflammation, oxidative stress, and apoptosis in colonic tissues which exhibits its potential for the management of UC.

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Section: Discussionmentioning

confidence: 99%

Sinapic Acid Ameliorates Acetic Acid-Induced Ulcerative Colitis in Rats by Suppressing Inflammation, Oxidative Stress, and Apoptosis

et al. 2022

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“…Moreover, PO can regulate the gut microbiota, promote probiotics and inhibit pathogenic bacteria ( 24 ). Several studies have shown that PO has strong immunomodulatory effects, improving T-helper (Th) 1/Th2 and Th2/regulatory T (Treg) balance and reducing Ig E levels, thus exerting anti-inflammatory effects ( 25 – 27 ).…”

Section: Discussionmentioning

confidence: 99%

Portulaca oleracea L. extracts alleviate 2,4-dinitrochlorobenzene-induced atopic dermatitis in mice

Huang

et al. 2022

Front. Nutr.

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Atopic dermatitis (AD) is a common chronic allergic skin disease characterized clinically by severe skin lesions and pruritus. Portulaca oleracea L. (PO) is a resourceful plant with homologous properties in medicine and food. In this study, we used two different methods to extract PO, and compared the therapeutic effects of PO aqueous extract (POAE) and PO ultrasound-assisted ethanol extract (POEE) on 2,4-dinitrochlorobenzene (DNCB)-induced AD mice. The results showed that in POAE and POEE, the extraction rates of polysaccharides were 16.95% and 9.85%, while the extraction rates of total flavonoids were 3.15% and 3.25%, respectively. Compared with AD mice, clinical symptoms such as erythema, edema, dryness and ulceration in the back and left ear were alleviated, and pruritus behavior was reduced after POAE and POEE treatments. The thickness of the skin epidermis was thinned, the density of skin nerve fibers labeled with protein gene product 9.5 (PGP9.5) was decreased, and mast cell infiltration was reduced. There was a decrease in blood lymphocytes, eosinophils and basophils, a significant decrease in spleen index and a noticeable decrease in serum immunoglobulin E (Ig E). POEE significantly reduced the concentration of the skin pruritic factor interleukin (Il)-31. POAE and POEE reduced the concentration of skin histamine (His), down-regulated mRNA expression levels of interferon-γ (Ifnγ), tumor necrosis factor-α (Tnf-α), thymic stromal lymphopoietin (Tslp) and Il-4, with an increase of Filaggrin (Flg) and Loricrin (Lor) in skin lesions. These results suggested that POAE and POEE may inhibit atopic response and alleviate the clinical symptoms of AD by inhibiting the expression of immune cells, inflammatory mediators and cytokines. PO may be a potential effective drug for AD-like diseases.

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“…[5,7,8] Notably, the dosage of natural phytochemical compounds can be flexibly adjusted according to the patients' symptoms, offering a highly personalized treatment approach. For instance, the ethanolic extract of Portulaca oleracea leaf, which contains two phenolic acids and five flavonoids, has been shown to effectively alleviate colitis symptoms through a wide range of mechanisms, including reducing the immune response, inhibiting histological injury, decreasing myeloperoxidase activity, and reducing fecal calprotectin levels; [9] aronia melanocarpa fruit juice rich in polyphenolic chemicals has been shown to dose-dependently ameliorate colitis and improve both macroscopic and microscopic signs of colitis, likely due to its antioxidant and anti-inflammatory properties; [10] dietary flavonoids such as kaempferol and fisetin have been demonstrated to effectively relieve intestinal inflammation, protect colonic mucosa, and provide promising nutraceutical alternatives for the treatment of colitis. [11,12] Unfortunately, poor water solubility and stability often limit the in vivo administration and bioavailability of many natural phytochemical compounds.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Potential Evaluation of Silk Sericin Stabilized Fisetin to Ulcerative Colitis

Wang,

Xiang,

et al. 2023

Macromolecular Bioscience

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Ulcerative colitis is a chronic inflammatory bowel disease with a high recurrence rate. Natural phytochemical compounds are increasingly being considered as preventative and supportive treatments for this condition. However, the poor water solubility and stability of many of these compounds limit their effectiveness in vivo. To address this issue, fisetin (FT), a natural phytochemical with poor solubility, was stabilized using silk sericin (SS) to create a composite (SS/FT). The therapeutic potential of the SS/FT on ulcerative colitis was extensively investigated, and the results showed that it effectively alleviated the body weight loss and colon length shortening induced by dextran sulfate sodium. Notably, SS/FT downregulated the immune response, decreased colonic histopathological lesions, and reduced the cGAS/STING signal activation. This suggests that SS/FT may offer a promising therapy for treating ulcerative colitis.This article is protected by copyright. All rights reserved

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Immunomodulatory assessment of Portulaca oleracea L. extract in a mouse model of colitis

Cited by 15 publications

References 56 publications

Sinapic Acid Ameliorates Acetic Acid-Induced Ulcerative Colitis in Rats by Suppressing Inflammation, Oxidative Stress, and Apoptosis

Sinapic Acid Ameliorates Acetic Acid-Induced Ulcerative Colitis in Rats by Suppressing Inflammation, Oxidative Stress, and Apoptosis

Portulaca oleracea L. extracts alleviate 2,4-dinitrochlorobenzene-induced atopic dermatitis in mice

Therapeutic Potential Evaluation of Silk Sericin Stabilized Fisetin to Ulcerative Colitis

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