Immunomodulatory activity of Melaleuca alternifolia concentrate (MAC): Inhibition of LPS-induced NF-κB activation and cytokine production in myeloid cell lines

Low, Pauline; Clark, Amanda M.; Chou, Tz-Chong; Chang, Tsu‐Chung; Reynolds, Maxwell; Ralph, Stephen John

doi:10.1016/j.intimp.2015.03.034

Cited by 20 publications

(14 citation statements)

References 46 publications

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“…On human peripheral blood cell lines, contrasting results were found. For three in vitro studies, similar concentrations 0.015% (Nogueira et al, ) and 0.016% (Brand et al, ; Low et al, ) showed no cytotoxic effects. Likewise, negligible effects were found for 0.1% (Caldefie‐Chézet et al, ).…”

Section: Resultsmentioning

confidence: 91%

“…alternifolia based treatments on inflammatory response and described important immunomodulatory events. Between its properties, cytokines modulation (Abe, Maruyama, Sekimoto, et al, ; Brand et al, ; Caldefie‐Chezet, Fusillier, Jarde, et al, ; Hart et al, ; Low et al, ; Nogueira, Aquino, Rossa Junior, & Spolidorio, ) and antioxidant activity (Brand, Ferrante, Prager, et al, ; Caldefie‐Chézet et al, ) were the main responsible for its anti‐inflammatory effect (Ninomiya, Hayama, Ishijima, et al, ). Its action through the suppression of the cytokines interleukin 1‐beta (IL‐1b), interleukin‐6 (IL‐6), interleukin‐10 (IL‐10) (Nogueira et al, ), tumoral necrosis factor (TNF‐α), and Prostaglandin E2 (Hart et al, ) was the most described.…”

Section: Resultsmentioning

confidence: 99%

“…alternifolia based treatments was contrasting on laboratory studies. Although some authors did not report cytotoxicity (Brand et al, ; Caldefie‐Chézet et al, ; Low et al, ; Nogueira et al, ), it was described by others (Caldefie‐Chezet et al, ; Hart et al, ). However, the toxicity to the cells increases in proportion to the concentration (Takarada et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…The reduction on the cytokine production by macrophage‐like cell lines basically sums up the M . alternifolia (0.03–0.0001%) activity (Low et al, ). These immuno‐inflammatory responses are shrunken by a cascade of different reactions produced by the organism.…”

Section: Discussionmentioning

confidence: 99%

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Melaleuca alternifoliaand its application against dental plaque and periodontal diseases: A systematic review

Casarin

Pazinatto

Santos

et al. 2017

Phytotherapy Research

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This is a systematic review of clinical and laboratory studies evaluating the effect of Melaleuca alternifolia on periodontopathogens, dental plaque, gingivitis, periodontitis, and inflammatory responses. The PubMed, Cochrane, Web of science, Bireme, Lilacs, Prospero, Open Grey, and Clinical Trials databases were searched to identify potentially eligible studies through October 2016. Of 1,654 potentially eligible studies, 25 were included in the systematic review. Their methodology was evaluated through the Cochrane Handbook for clinical studies and the GRADE system for in vivo/in vitro studies. Although clinical studies must be interpreted with caution due to methodological limitations, laboratory studies have found promising results. In vitro evidences showed that M. alternifolia has bactericidal and bacteriostatic effects against the most prevalent periodontopathogens. Clinical studies found comparable effects to chlorhexidine 0.12% in reducing gingival inflammation, although the antiplaque effect was lower. M. alternifolia also showed antioxidant properties, which are beneficial to the host, allied to the reduction on immune-inflammatory responses to pathogens. This systematic review suggests that the M. alternifolia has potential anti-inflammatory and antimicrobial properties, which can be easily applied to the periodontal tissues. However, further clinical trials are needed to elucidate the clinical relevance of its application.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Melaleuca alternifoliaand its application against dental plaque and periodontal diseases: A systematic review

Casarin

Pazinatto

Santos

et al. 2017

Phytotherapy Research

View full text Add to dashboard Cite

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“…The EO concentrations evaluated were as follows: 0.0 – control, 1.0, 2.0, 4.0, 8.0 and 16.0 μ g/ml. These concentrations were chosen based on literature data (Manosroi et al., ; de Almeida et al., ; Kumar et al., ; Santin et al., ; Kpoviessi et al., ; Nogueira et al., ; Low et al., ) and preliminary cytotoxicity studies of the EOs on red drum head‐kidney macrophages by trypan blue exclusion test (Valadares et al., ), as well as by examining haemolytic activity of the EOs against red drum red blood cells as described by Rodrigues et al. ().…”

Section: Methodsmentioning

confidence: 99%

In vitro effects of plant essential oils on non‐specific immune parameters of red drum, Sciaenops ocellatus L.

Sutili

Gatlin

Rossi

et al. 2016

Animal Physiology Nutrition

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Phytochemicals such as plant essential oils (EOs) have been reported to favour various activities in the innate immune system of fish. Thus, the aim of this study was to verify the in vitro effect of three different plant EOs (Ocimum americanum, Cymbopogon flexuosus and Melaleuca alternifolia) on non-specific immune parameters and erythrocyte osmotic fragility of red drum, Sciaenops ocellatus. Concentrations of each plant EO evaluated in preparations of head-kidney macrophages, blood leucocytes and blood plasma were as follows: 0.0 (control), 1.0, 2.0, 4.0, 8.0, and 16.0 μg/ml. Red drum head-kidney macrophages significantly increased extracellular superoxide anion production when exposed (20 h) to O. americanum EO (1.0-8.0 μg/ml) and C. flexuosus EO (2.0 and 4.0 μg/ml). The respiratory burst of blood leucocytes (NBT test) significantly increased in all concentrations when compared to the respective control group, for all EOs. At the highest concentration (16.0 μg/ml), C. flexuosus EO significantly inhibited the haemolytic activity of complement system in red drum blood after 1 h exposure. None of the tested concentrations significantly altered plasma lysozyme activity or erythrocyte osmotic fragility after exposing (1 h) red drum whole blood to each EO. This study demonstrated that these plant EOs are capable of triggering superoxide anion production in red drum leucocytes (head-kidney macrophages and/or blood leucocytes). In vivo studies are warranted to address their potential as immunostimulants in the diet of red drum and other aquacultured species.

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Intratumoral pro-oxidants promote cancer immunotherapy by recruiting and reprogramming neutrophils to eliminate tumors

Ralph

Reynolds²

2022

Cancer Immunol Immunother

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Neutrophils have recently gained recognition for their potential in the fight against cancer. Neutrophil plasticity between the N1 anti-tumor and N2 pro-tumor subtypes is now apparent, as is the ability to polarize these individual subtypes by interventions such as intratumoral injection of various agents including bacterial products or pro-oxidants. Metabolic responses and the production of reactive oxygen species (ROS) such as hydrogen peroxide act as potent chemoattractants and activators of N1 neutrophils that facilitates their recruitment and ensuing activation of a toxic respiratory burst in tumors. Greater understanding of the precise mechanism of N1 neutrophil activation, recruitment and regulation is now needed to fully exploit their anti-tumor potential against cancers both locally and at distant sites. This systematic review critically analyzes these new developments in cancer immunotherapy.

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Immunomodulatory activity of Melaleuca alternifolia concentrate (MAC): Inhibition of LPS-induced NF-κB activation and cytokine production in myeloid cell lines

Cited by 20 publications

References 46 publications

Melaleuca alternifoliaand its application against dental plaque and periodontal diseases: A systematic review

Melaleuca alternifoliaand its application against dental plaque and periodontal diseases: A systematic review

In vitro effects of plant essential oils on non‐specific immune parameters of red drum, Sciaenops ocellatus L.

Intratumoral pro-oxidants promote cancer immunotherapy by recruiting and reprogramming neutrophils to eliminate tumors

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