Immunomodulatory Activity of a Traditional Sri Lankan Concoction of Coriandrum sativum L. and Coscinium fenestratum G.

Abstract

Objective. To investigate the immunomodulatory activity of a traditional Sri Lankan concoction of Coriandrum sativum L. and Coscinium fenestratum (Gaertn.) Colebr., which is a Sri Lankan traditional medicine used to relieve inflammation and cold. Methods. In vivo anti-inflammatory activity was tested using carrageenan-induced rat paw-edema model. Mechanism of anti-inflammatory activity was assessed by investigating the production of nitric oxide (NO), expression of iNOS enzyme, and reactive oxygen species (ROS) by rat peritoneal cells. The membrane stabilizing activity was also tested. The antibody response was determined by assessing the specific haemagglutination antibodies raised against sheep red blood cells. Results. The three doses of freeze-dried concoction used ((human equivalent dose (HED)—183 mg/kg) 2 × HED and 1/2HED; n = 6 rats/group) showed significant inhibition of paw edema compared to water control at 3rd–5th hours (p<0.05). Both HED and 1/2HED exhibited marked anti-inflammatory activity (72–83% inhibition at 4th-5th hours; p<0.05). The HED of the concoction showed significant inhibition of NO (77.5 ± 0.73%, p<0.001) and ROS production (26.9 ± 2.55%; p<0.01) by rat peritoneal cells. Inhibition of NO production in the concoction treated rat peritoneal cells was confirmed by the lack of iNOS expression. The concoction also exhibited significant membrane stabilizing activity (IC50 = 0.0006 μg/ml; p=0.001). HED resulted in a significantly high induction of specific antibody production against SRBC antigens as detected by SRBC haemagglutination assay (mean day 14 titers 253.3 compared to control: 66.7) (p<0.01). Conclusions. The traditional Sri Lankan concoction of C. sativum and C. fenestratum demonstrated potent in vivo anti-inflammatory activity, significant reduction of ROS, and NO production by rat peritoneal cells and the lack of iNOS expression confirmed the low NO production. The increased membrane stability also supports the anti-inflammatory activity of the concoction. Further, this concoction induced a significantly high antibody response reflecting its immunostimulatory activity. Together these results scientifically validate the therapeutic use of the concoction of C. sativum and C. fenestratum in Sri Lankan traditional medicinal system for immunomodulatory effects.

Leptadenia Pyrotechnica Aqueous Extract Suppresses Innate Immunity and Enhances Adaptive Immunity in Rats

“…To give more proof to this finding is the unpublished finding of higher lymphocytes count for the LD group, but not for the HD group, compared with the count for the control. The present findings agree with the previous studies on healthy rats administered with methanolic leaf extract of Moringa oleifera, [33] and the concoction of Coriandrum sativum and Coscinium fenestratum [38], and on mice using an extract of zapoteca portoricensis [34] which showed a significantly higher mean haemagglutinating antibody titer to sheep red blood cells. The current results are in disagreement with a previous study [39] in healthy mice administered with the aqueous extract of Stachytarpetha jamaicensis, which resulted in significantly higher haemagglutinating antibody titer compared with the control group.…”

Increased expression of co-stimulatory molecules and enhancement of the IgG response in rats orally administered with a polyherbal formulation

“…However, ELISAs to determine the specific IgM and IgG antibody concentrations raised against immunogens such as keyhole limpet hemocyanin (KLH) [22] or BSA [23] have became the preferred assays to measure the humoral immunity [ 24 ]. Our previous studies have also used the hemagglutination assay [ 9 ] or ELISA (unpublished data) for the detection of antibodies against SRBC to describe the immunostimulatory activity of the concoction of C. sativum and C. fenestratum . The present study deployed the use of ELISA to determine anti-BSA specific IgM and IgG responses to demonstrate the immunostumulatory activity of LS.…”

“…Different medicinal plants and combinations have been shown to exhibit immunostimulatory activity including several formulations and concoctions which contain some of the ingredients in LS. A recent study on a Sri Lankan traditional concoction consisting two ingredients found in LS, ie, Coriandrum sativum and Coscinium fenestratum had demonstrated its immunostimulatory activity [ 9 ]. Other studies have demonstrated the effect of different A yurvedic preparations/polyherbal formulations on immunomodulation/immunostimulation using animal models.…”

A Review on Herbal Medicinal plants as Natural Immunomodulators from the Indian Kitchen

“…In vivo anti-inflammatory and immunostimulatory activity of Coriandrum sativu was studied using carrageenan-induced rat paw edema model which showed significant inhibition of nitric oxide, membrane stabilizing activity, superoxide production, and production of antibody [15] . Methanol extracts of Coriandrum sativum made from leaves and seeds were analyzed.…”