Immunomodulatory activity of a fructooligosaccharide isolated from burdock roots

Zhang, Xiujuan; Liu, Shao-fang; Lü, Yan; Wang, Jian-yue; Chen, Kaoshan

doi:10.1039/c8ra10091h

Cited by 13 publications

(9 citation statements)

References 44 publications

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“…Although a water‐soluble GFn inulin‐type polysaccharide (ALP‐1) purified from A. lappa was found by Wang et al 23 to alleviate mouse colitis, this polysaccharide had a molecular weight of 5120 Da and possessed branched chains, differing from the polysaccharide used in the present study, which may be related to different extraction methods 24 . Our previous research had found that BFO effectively improved the immunological function of S180 tumor‐bearing mice 18 . Herein, we aimed to investigate the preventive effects of BFO in colitis and elucidate the underlying mechanism.…”

Section: Introductionmentioning

confidence: 68%

“…All mice were allowed to freely drink the respective solutions for 42 days. The selection of BFO concentration was based on our preliminary experiment and previous research 18,28 …”

Section: Methodsmentioning

confidence: 99%

“…24 Our previous research had found that BFO effectively improved the immunological function of S180 tumor-bearing mice. 18 Herein, we aimed to investigate the preventive effects of BFO in colitis and elucidate the underlying mechanism.…”

mentioning

confidence: 99%

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Long‐term oral administration of burdock fructooligosaccharide alleviates DSS‐induced colitis in mice by mediating anti‐inflammatory effects and protection of intestinal barrier function

Ma,

Zhang,

et al. 2023

Immunity Inflam & Disease

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BackgroundUlcerative colitis, a typical subtype of inflammatory bowel disease, can cause many serious complications. Burdock fructooligosaccharide (BFO), a linear inulin with a purity of 99.439% and a molecular weight of 2345 Da, demonstrates anti‐inflammatory and immunomodulatory properties.MethodsThe Kunming mice were divided into two experimental models: a normal pretreatment model and a colitis experimental model. During the experimental treatment period, we assessed changes in weight and disease activity index (DAI), quantified the intestinal index, and determined myeloperoxidase (MPO) activity and reactive oxide species (ROS) levels in colitis mice. We also photographed colon morphology to investigate alterations in the integrity of the intestinal barrier function. Finally, we performed ELISA and qRT‐PCR to evaluate the anti‐inflammatory effect of BFO treatment on colitis mice.ResultThe long‐term oral administration of BFO alone exhibited protective effects by preventing disruption of the intestinal functional structure and increasing the colon index in mice. However, in a dextran sodium sulfate (DSS)‐induced colitis mouse model, BFO administration facilitated quick recovery of body weight and effectively reduced the DAI, especially in the BFO‐H group (500 mg/kg/day). BFO treatment maintained the integrity of the intestinal barrier by attenuating the crypt distortion and increasing the goblet cells count It restored the DSS‐induced colon shortening and reduced the symptoms of colitis. These effects may be attributed to the appropriate concentrations of BFO effectively inhibiting MPO activity, clearing excessive ROS, and relieving spleen abnormalitie. BFO also attenuated the overexpression and excessive secretion of inflammatory cytokines (TNF‐α, IL‐1β, IL‐6, and MCP‐1) induced by DSS, reduced intestinal inflammation, and consequently protected the intestinal barrier function.ConclusionBFO effectively alleviated the symptoms of DSS‐induced colitis by mediating anti‐inflammatory effects and protecting the intestinal barrier integrity, thereby potentially facilitating the utilization of safer and more efficacious polysaccharides for managing chronic inflammatory diseases.

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Section: Introductionmentioning

confidence: 68%

“…All mice were allowed to freely drink the respective solutions for 42 days. The selection of BFO concentration was based on our preliminary experiment and previous research 18,28 …”

Section: Methodsmentioning

confidence: 99%

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Long‐term oral administration of burdock fructooligosaccharide alleviates DSS‐induced colitis in mice by mediating anti‐inflammatory effects and protection of intestinal barrier function

Ma,

Zhang,

et al. 2023

Immunity Inflam & Disease

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“…A recent study revealed the immune‐regulatory and anticancerous effect of FOS derived from Arctium lappa root on S180 tumor‐bearing mice model. A dose of 1000 mg kg −1 day −1 of A. lappa FOS inhibited the tumor up to 44% (Zhang et al., 2019). Another noteworthy contribution unraveled the colon cancer inhibition upon fructan administration involved tumor necrosis factor alpha (TNFα) (Rivera‐Huerta et al., 2017).…”

Section: Resultsmentioning

confidence: 99%

Purification of Aspergillus tamarii mycelial fructosyltransferase (m‐FTase), optimized FOS production, and evaluation of its anticancer potential

Choukade

Kango

2022

Journal of Food Science

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In the present study, generation of prebiotic fructooligosaccharides (FOS) using Aspergillus tamarii FTase was optimized by applying response surface methodology. Optimal FOS (251 g L −1 ) was generated at 28.4 • C, pH 7.0 and 50% (w/v) sucrose leading to 1.97-fold yield enhancement. The m-FTase was purified using ultrafiltration followed by HiTrap Q HP anion exchange chromatography resulting in 2.15-fold purified FTase with 12.76 U mg -1 specific activity. Purified FTase (75 kDa) had K m and V max values of 1049.717 mM and 2.094 µmol min -1 mg -1 , respectively. FOS incorporation led to upregulation of caspase 3, caspase 9, and Bax genes suggesting mitochondrial apoptosis activation in cancer cells. The study describes characteristics of purified FTase from A. tamarii, production optimization of FOS and unravels the role of FOS in anticancer activity against HT-29 cells.

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“…Protocols with details for proliferation assays are detailed in Supplement 2. Further, the 6-CFDA test [25], acid phosphatase test [26], DAF-2 DA intracellular nitric oxide test [27], LDH in media test [17], MTT test [28], xCELLigence real time cell analysis were used [29].…”

Section: Proliferation and Viability Assaysmentioning

confidence: 99%

The cAMP Inducers Modify N-Acetylaspartate Metabolism in Wistar Rat Brain

Kowalski¹,

Pikuł

Lewandowski

et al. 2021

Antioxidants

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Neuronal N-acetylaspartate production appears in the presence of aspartate N-acetyltransferase (NAT8L) and binds acetyl groups from acetyl-CoA with aspartic acid. Further N-acetylaspartate pathways are still being elucidated, although they seem to involve neuron-glia crosstalk. Together with N-acetylaspartate, NAT8L takes part in oligoglia and astroglia cell maturation, myelin production, and dopamine-dependent brain signaling. Therefore, understanding N-acetylaspartate metabolism is an emergent task in neurobiology. This project used in in vitro and in vivo approaches in order to establish the impact of maturation factors and glial cells on N-acetylaspartate metabolism. Embryonic rat neural stem cells and primary neurons were maturated with either nerve growth factor, trans-retinoic acid or activators of cAMP-dependent protein kinase A (dibutyryl-cAMP, forskolin, theophylline). For in vivo, adult male Wistar rats were injected with theophylline (20 mg/kg b.w.) daily for two or eight weeks. Our studies showed that the N-acetylaspartate metabolism differs between primary neurons and neural stem cell cultures. The presence of glia cells protected N-acetylaspartate metabolism from dramatic changes within the maturation processes, which was impossible in the case of pure primary neuron cultures. In the case of differentiation processes, our data points to dibutyryl-cAMP as the most prominent regulator of N-acetylaspartate metabolism.

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Immunomodulatory activity of a fructooligosaccharide isolated from burdock roots

Abstract: Immunomodulatory activity of burdock fructooligosaccharide (BFO-1) on immune cells in in vitro normal mice, immunosuppressed mice treated with cyclophosphamide and S180 tumor-bearing mice.

Cited by 13 publications

References 44 publications

Long‐term oral administration of burdock fructooligosaccharide alleviates DSS‐induced colitis in mice by mediating anti‐inflammatory effects and protection of intestinal barrier function

Long‐term oral administration of burdock fructooligosaccharide alleviates DSS‐induced colitis in mice by mediating anti‐inflammatory effects and protection of intestinal barrier function

Purification of Aspergillus tamarii mycelial fructosyltransferase (m‐FTase), optimized FOS production, and evaluation of its anticancer potential

The cAMP Inducers Modify N-Acetylaspartate Metabolism in Wistar Rat Brain

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