Immunomodulatory actions of antibiotics

Minic, Svetlana; Bojic, Milena; Vukadinov, J; Canak, G; Fabri, Milotka; Bojić, I

doi:10.2298/mpns0908327m

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…Antibiotics treatment significantly eliminates the bacterial load and inhibits the formation of TPE. Although antibiotics are most widely known for their direct microbicidal activity 12 , 13 , increasing evidence suggests that many antibiotics also modulate activities of immune cells 14 . For example, rifampicin is known to induce the expression of CD1 in human T cells and monocytes and therefore immune responses to various infections 15 , 16 .…”

Section: Introductionmentioning

confidence: 99%

Antibiotics induce polarization of pleural macrophages to M2-like phenotype in patients with tuberculous pleuritis

Wang

Zhang

Sui

et al. 2017

Sci Rep

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Pleural macrophages play critical roles in pathogenesis of tuberculous pleuritis, but very little is known about their response to anti-tuberculosis antibiotics treatment. Here, we examined whether and how pleural macrophages change in phenotype, transcription and function following antibiotics treatment in patients with tuberculous pleuritis. Results show pro-inflammatory cytokines were down-regulated significantly post antibiotic treatment in the pleural effusions and pleural macrophages up-regulated markers characteristic of M2 macrophages such as CD163 and CD206. Differential expression analysis of transcriptomes from four paired samples before and after treatment identified 230 treatment-specific responsive genes in pleural macrophages. Functional analysis identified interferon-related pathway to be the most responsive genes and further confirmed macrophage polarization to M2-like phenotype. We further demonstrate that expression of a significant fraction of responsive genes was modulated directly by antibiotics in pleural macrophages in vitro. Our results conclude that pleural macrophages polarize from M1-like to M2-like phenotype within a mean of 3.5 days post antibiotics treatment, which is dependent on both pleural cytokine environment and direct modulatory effects of antibiotics. The treatment-specific genes could be used to study the roles of pleural macrophages in the pathogenesis of tuberculous pleuritis and to monitor the response to antibiotics treatment.

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Section: Introductionmentioning

confidence: 99%

Antibiotics induce polarization of pleural macrophages to M2-like phenotype in patients with tuberculous pleuritis

Wang

Zhang

Sui

et al. 2017

Sci Rep

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“…Hirurški zatvorena oroantralna komunikacija vestibularnim mukoperiostalnim režnjem (Rehrmann) Fig. Antibiotici omogućavaju pozitiv ne imunomodulatorne reakcije u organizmu (Slika 2) [5]. Surgically closed oroantral communication by vestibular mucoperiosteal slice (Rehrmann) do hirurške intervencije.…”

Section: Uvodunclassified

Stimulation of mucoperiostal slice epithelization by small power laser after the primary plastic of oroantral communication

Jovanović¹,

Burić²,

Tijanić³

2010

Med pregl

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“…Depending on their biochemical structure and mechanisms of action, they can act not only as bactericidal compounds but may also influence neutrophil or lymphocyte functions [ 6 , 7 ]. Moreover, they can influence release of cytokines or delayed-type hypersensitivity reactions [ 6 , 8 , 9 ]. However, the influence of antibiotics on NET release is largely unknown.…”

Section: Introductionmentioning

confidence: 99%

The effect of clindamycin and amoxicillin on neutrophil extracellular trap (NET) release

Bystrzycka

Moskalik

Sieczkowska

et al. 2016

cejoi

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Neutrophil extracellular traps (NETs) are threads of nuclear DNA complexed with antimicrobial proteins released by neutrophils to extracellular matrix to bind, immobilise, and kill different pathogens. NET formation is triggered by different physiological and non-physiological stimulants. It is also suggested that antibiotics could be non-physiological compounds that influence NET release.The aim of the study was to investigate the effect of clindamycin and amoxicillin on NET release and the phagocyte function of neutrophils.Neutrophils isolated from healthy donors by density centrifugation method were incubated with amoxicillin or clindamycin for two hours, and then NET release was stimulated with phorbol 12-myristate 13-acetate (PMA). After three hours of incubation with PMA NETs were quantified as amount of extracellular DNA by fluorometry and visualised by immunofluorescent microscopy. The percent of phagocyting cells was measured by flow cytometry.We showed that amoxicillin induces NET formation (increase of extracellular DNA fluorescence, p = 0.03), while clindamycin had no influence on NET release (p > 0.05), as confirmed by quantitative measurement and fluorescent microscopy. Regarding phagocyte function, both antibiotics increased bacterial uptake (43.3% and 61.6% median increase for amoxicillin and clindamycin, respectively).We concluded that the ability of antibiotics to modulate NET release depends on the antibiotic used and is not associated with their ability to influence phagocytosis.

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Immunomodulatory actions of antibiotics

Abstract: There is still no clear standing on real effects of antibiotics on the immune system. Clinicians should search for more information from this new-old field of investigation in order to give more adequate therapy to patients.

Cited by 8 publications

References 12 publications

Antibiotics induce polarization of pleural macrophages to M2-like phenotype in patients with tuberculous pleuritis

Antibiotics induce polarization of pleural macrophages to M2-like phenotype in patients with tuberculous pleuritis

Stimulation of mucoperiostal slice epithelization by small power laser after the primary plastic of oroantral communication

The effect of clindamycin and amoxicillin on neutrophil extracellular trap (NET) release

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