Immunomodulation of Mytilus hemocytes by individual estrogenic chemicals and environmentally relevant mixtures of estrogens: In vitro and in vivo studies

Canesi, Laura; Lorusso, Lucia Cecilia; Ciacci, Caterina; Betti, Michele; Rocchi, Marco; Pojana, Giulio; Marcomini, Antonio

doi:10.1016/j.aquatox.2006.10.010

Cited by 108 publications

(46 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The MAP-kinase pathway is important in embryogenesis, cell growth, differentiation and apoptosis, and disturbances of STAT signalling favour tumour development by compromising the immune surveillance. Thus the findings of Canesi et al (2007a) may have significant implications for mussel health. Canesi et al (2007b) also reported altered patterns of gene expression and activity of enzymes involved in the redox balance following an injection of BPA into the posterior adductor muscle (at nominal doses of 3, 15 and 60 ng BPA g 21 dry wt.).…”

mentioning

confidence: 99%

“…Canesi et al (2005) showed that BPA at a nominal concentration of 25 nM (5.7 mg BPA l 21 ) in the haemolymph caused a significant lysosomal membrane destabilization in haemocytes in M. galloprovincialis. Canesi et al (2007a) evaluated the lysosomal membrane destabilization in extracted haemocytes of the same species and reported an NOEC of 1 mM and a corresponding EC 50 value of 34.5 mM (7.87 mg l…”

mentioning

confidence: 99%

See 1 more Smart Citation

A critical analysis of the biological impacts of plasticizers on wildlife

Oehlmann

Schulte‐Oehlmann

Kloas

et al. 2009

Phil. Trans. R. Soc. B

721

341

View full text Add to dashboard Cite

This review provides a critical analysis of the biological effects of the most widely used plasticizers, including dibutyl phthalate, diethylhexyl phthalate, dimethyl phthalate, butyl benzyl phthalate and bisphenol A (BPA), on wildlife, with a focus on annelids (both aquatic and terrestrial), molluscs, crustaceans, insects, fish and amphibians. Moreover, the paper provides novel data on the biological effects of some of these plasticizers in invertebrates, fish and amphibians. Phthalates and BPA have been shown to affect reproduction in all studied animal groups, to impair development in crustaceans and amphibians and to induce genetic aberrations. Molluscs, crustaceans and amphibians appear to be especially sensitive to these compounds, and biological effects are observed at environmentally relevant exposures in the low ng l 21 to mg l 21 range. In contrast, most effects in fish (except for disturbance in spermatogenesis) occur at higher concentrations. Most plasticizers appear to act by interfering with the functioning of various hormone systems, but some phthalates have wider pathways of disruption. Effect concentrations of plasticizers in laboratory experiments coincide with measured environmental concentrations, and thus there is a very real potential for effects of these chemicals on some wildlife populations. The most striking gaps in our current knowledge on the impacts of plasticizers on wildlife are the lack of data for long-term exposures to environmentally relevant concentrations and their ecotoxicity when part of complex mixtures. Furthermore, the hazard of plasticizers has been investigated in annelids, molluscs and arthropods only, and given the sensitivity of some invertebrates, effects assessments are warranted in other invertebrate phyla.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

A critical analysis of the biological impacts of plasticizers on wildlife

Oehlmann

Schulte‐Oehlmann

Kloas

et al. 2009

Phil. Trans. R. Soc. B

721

341

View full text Add to dashboard Cite

show abstract

“…Phagocytosis is the predominant mechanism of cellular defence, which, in bivalves, is mainly mediated by circulating and tissue haemocytes (Pipe and Coles, 1995;Pipe et al, 1999). This process proceeds through a number of well-defined stages: recognition, chemotaxis, attachment, respiratory burst, ingestion, and destruction (Galloway and Goven, 2006); and has been focused on as biomarkers for immunotoxic effects in a number of studies (Bussell et al, 2008;Canesi et al, 2007;Pipe et al, 1999;Reid et al, 2003). Here, phagocytic activity (uptake of neutral red stained zymosan particles) and apoptosis of haemocytes (caspase 3/7 activity) were analysed as cellular and haemolytic activity (lysis of erythrocytes) as humoral endpoints.…”

Section: Introductionmentioning

confidence: 99%

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Höher

Köhler

Strand

et al. 2012

Marine Environmental Research

View full text Add to dashboard Cite

a b s t r a c tThe Baltic Sea is a semi-enclosed sea with a steady salinity gradient (3&e30&). Organisms have adapted to such low salinities, but are suspected to be more susceptible to stress. Within the frame of the integrated environmental monitoring BONUS þ project "BEAST" the applicability of immune responses of the blue mussel was investigated in Danish coastal waters. The sampling sites were characterised by a salinity range (11e19&) and different mixtures of contaminants (metals, PAHs and POPs), according to chemical analysis of mussel tissues. Variation partitioning (redundancy analysis) was applied to decompose salinity and contamination effects. The results indicated that cellular immune responses (total and differential haemocyte count, phagocytic activity and apoptosis) were mainly influenced by contaminants, whereas humoral factors (haemolytic activity) were mainly impacted by salinity. Hence, cellular immune functions may be suitable as biomarkers in monitoring programmes for the Baltic Sea and other geographic regions with salinity variances of the studied range.Crown

show abstract

“…However, some studies have demonstrated that E2 can disrupt non-reproductive endocrine events, namely the stress responses in fish (Teles et al 2005;Maria et al 2008;Moura Costa et al 2010). The immune system of molluscs also represents a significant target for the action of environmental estrogens, as E2 in the nM range (1-100 nM) induces lysosomal destabilisation in Mytilus (Canesi et al 2007). Exposure to E2 can also disturb the intra-cellular environment, increasing the production of reactive oxygen species (ROS), thereby impairing the antioxidant defence system (Maria et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant enzymes responses of polychaete Perinereis aibuhitensis following chronic exposure to 17β-estradiol

Dong

et al. 2016

Italian Journal of Animal Science

View full text Add to dashboard Cite

The occurrence of 17b-estradiol (E2) in the aquatic environment can lead to damage to the reproductive system, along with other adverse effects including oxidative stress, in aquatic organisms. In the present study, Perinereis aibuhitensis were treated with E2 at 0.1, 1, 10, 100 and 1000 lg/L for 205 d, after which the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), and the concentrations of glutathione antioxidant biomarker responses were studied. Weight gain and specific growth rate of P. aibuhitensis were not significantly affected by E2 treatment. Although no significant differences in mortality were observed, the group receiving the highest dose of E2 (1000 lg/L) experienced the highest rate of mortality. Treatment with E2 enhanced the levels of total glutathione (T-GSH), but levels of glutathione reduced (GSH) were significantly decreased by 17.02, 20.55, 23.45, 31.91 and 56.08%, with a concomitant increase in the levels of glutathione disulphide (GSSG) by 148.58, 213.05, 232.62, 294.63 and 306.45%, at 0.1, 1, 10, 100 and 1000 lg/L E2, respectively. The redox ratio of GSH/GSSG was significantly decreased (p < 0.01). The results of this study suggest that long-term exposure of P. aibuhitensis to E2 may inhibit antioxidant enzyme activities, thereby reducing the capacity of its detoxification system. ARTICLE HISTORY

show abstract

Immunomodulation of Mytilus hemocytes by individual estrogenic chemicals and environmentally relevant mixtures of estrogens: In vitro and in vivo studies

Cited by 108 publications

References 50 publications

A critical analysis of the biological impacts of plasticizers on wildlife

A critical analysis of the biological impacts of plasticizers on wildlife

Effects of various pollutant mixtures on immune responses of the blue mussel (Mytilus edulis) collected at a salinity gradient in Danish coastal waters

Antioxidant enzymes responses of polychaete Perinereis aibuhitensis following chronic exposure to 17β-estradiol

Contact Info

Product

Resources

About