Immunomodulation by Bioprocessed Polysaccharides from &amp;lt;i&amp;gt;Lentinus edodes&amp;lt;/i&amp;gt; Mycelia Cultures with Rice Bran in the &amp;lt;i&amp;gt;Salmonella&amp;lt;/i&amp;gt; Gallinarum-infected Chicken Macrophages

Tae, Lee Hyung; Jong, Lee Sang; Yoon, Jang Won

doi:10.13103/jfhs.2018.33.5.383

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…In previous studies on FLE products, analysis of the structure and content of constituents of FLE mushroom (Xue, Ma, et al., 2020), analysis of the bio‐active capacity of FLE‐derived polysaccharides (Kim, Kim, et al., 2007), food development (Jung et al., 2016; Kim, Kim, et al., 2007), and evaluation of biological activities, including anti‐cancer (Liu et al., 2009; Zhong et al., 2012), anti‐skin photoaging (Choi et al., 2019), and anti‐obesity effects (Kim, Lee, et al, 2017), have been performed. In particular, several studies have recently described the development of LE‐fermented food materials using apple or pear pomace, rice bran (Kim et al., 2019; Lee et al., 2018), and LE‐fermented beverages and foods using black rice ( Oryza sativa Linne) (Park et al., 2012; Zhang et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity, metabolic enzyme inhibitory, and anti‐inflammatory effect of Lentinula edodes fermented using probiotic lactobacteria

et al. 2021

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We found that the fermented Lentinula edodes (FLE) products exhibited various differences in terms of proximate composition, free sugar, and amino acid. In particular, there were higher levels of ergosterol, and ergothioneine in FLE‐Pediococcus pentosaceus (PP) and ‐Lactobacillus acidophilus (LA) than in the L. edodes (LE) products. The survival rates of lactic acid bacteria (LAB) strains on artificial gastric juice, artificial bile, or heat (50–60°C) were observed to vary from 60%–66%, 60%–66%, to 42%–79%, respectively. The FLE products up to 300 μg/ml had no cytotoxicity on RAW264.7, AGS, and RBL‐2H3 cells, but inhibited the activities of α‐amylase, α‐glucosidase, and pancreatic lipase, as well as the production of nitrite, IL‐1β, IL‐4, TNF‐α, and prostaglandin E2 (PGE2) from lipopolysaccharide (LPS)‐induced inflammatory response. Our findings suggest that FLE products have metabolic enzyme inhibitory and anti‐inflammatory effects. Practical applications Fermentation plays a critical role in improving the functional and nutritional properties of food. In addition, lactobacteria are the main microorganisms involved in the fermentation of food known to have a variety of biological activities. Therefore, the utilization of lactobacteria for research and development of mushroom food materials can be used as a key strategy to improve the biological activity characteristics of mushroom food materials and to increase their active ingredient content. The present results show that FLE products had promising inhibitory efficacies against the activities of obesity‐related metabolic enzymes and LPS‐induced inflammatory response. These suggest that FLE products have the potential to be developed as functional probiotic dietary supplements or food products.

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Section: Discussionmentioning

confidence: 99%

Cytotoxicity, metabolic enzyme inhibitory, and anti‐inflammatory effect of Lentinula edodes fermented using probiotic lactobacteria

et al. 2021

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Immunostimulatory Effect of Rice Bran Fermented by Lentinus edodes Mycelia on Mouse Macrophages and Splenocytes

Kang¹,

Yang²,

Lee

et al. 2022

JKFN

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Rice bran, a by-product of the rice dehulling process, contains bioactive polysaccharides which have immunomodulatory activity. We prepared fermented rice bran with Lentinus edodes mycelia [Erom's fermented rice bran (EFR)] to extract the polysaccharides and improve the immune-enhancing properties. The objective of this study was to evaluate the in vitro and ex vivo immunostimulatory effects of EFR on mouse macrophages and splenocytes. We investigated the effects of EFR on the production of nitric oxide (NO) and cytokines using the Griess assay and enzyme linked immunosorbent assay (ELISA), while the expression of inducible nitric oxide (iNOS) and cytokines in RAW264.7 cells was measured using reverse transcription-polymerase chain reaction (RT-PCR), Additionally, peritoneal macrophages and splenocytes were isolated from C57/BL6 mice. The macrophage lysosomal enzyme activity and splenocyte proliferation were determined using a lysosomal acid phosphatase assay and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, respectively. In vitro studies showed that EFR significantly increased the production of NO and cytokines [interleukin (IL)-6, IL-1β, IL-10, and tumor necrosis factor-α], and up-regulated the mRNA expression of iNOS and cytokines in RAW264.7 cells. Ex vivo studies revealed that EFR increased splenocyte proliferation and lysosomal enzyme activity of peritoneal macrophages. These findings indicate that EFR could enhance immunity by inducing macrophage activation and splenocyte proliferation. Therefore, EFR has potential as a source material for functional foods that boost the immune system.

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Immunomodulation by Bioprocessed Polysaccharides from <i>Lentinus edodes</i> Mycelia Cultures with Rice Bran in the <i>Salmonella</i> Gallinarum-infected Chicken Macrophages

Cited by 2 publications

References 27 publications

Cytotoxicity, metabolic enzyme inhibitory, and anti‐inflammatory effect of Lentinula edodes fermented using probiotic lactobacteria

Cytotoxicity, metabolic enzyme inhibitory, and anti‐inflammatory effect of Lentinula edodes fermented using probiotic lactobacteria

Immunostimulatory Effect of Rice Bran Fermented by Lentinus edodes Mycelia on Mouse Macrophages and Splenocytes

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