Immunomodulating Activity of Aronia melanocarpa Polyphenols

Ho, Giang Thanh Thi; Bräunlich, Marie; Austarheim, Ingvild; Wangensteen, Helle; Malterud, Karl Egil; Slimestad, Rune; Barsett, Hilde

doi:10.3390/ijms150711626

Cited by 38 publications

(28 citation statements)

References 27 publications

(37 reference statements)

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“…Trimeric procyanidin C1 (IC 50 19.4 ± 2.1 µM) possessed stronger complement fixating activity compared to the dimeric procyanidins B2 (IC 50 70.6 ± 4.5 µM) and B5 (IC 50 65.0 ± 3.1 µM), while the monomers epicatechin and catechin were almost inactive (>200 µM). These results are in agreement with those reported previously [27,28]. Due to the fact that anthocyanins are metabolized to simpler phenolic substances, it was also of interest to investigate whether these metabolites that are detected in urine, feces and plasma after consumption of elderberries could have complement fixating activity.…”

Section: Resultssupporting

confidence: 92%

Elderberry and Elderflower Extracts, Phenolic Compounds, and Metabolites and Their Effect on Complement, RAW 264.7 Macrophages and Dendritic Cells

Wangensteen

Barsett

2017

IJMS

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Modulation of complement activity and inhibition of nitric oxide (NO) production by macrophages and dendritic cells may have therapeutic value in inflammatory diseases. Elderberry and elderflower extracts, constituents, and metabolites were investigated for their effects on the complement system, and on NO production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages and murine dendritic D2SC/I cells. The EtOH crude extracts from elderberry and elderflower and the isolated anthocyanins and procyanidins possessed strong complement fixating activity and strong inhibitory activity on NO production in RAW cells and dendritic cells. Phenolic compounds in the range of 0.1-100 µM showed a dose-dependent inhibition of NO production, with quercetin, rutin, and kaempferol as the most potent ones. Among the metabolites, caffeic acid and 3,4-dihydroxyphenylacetic acid showed the strongest inhibitory effects on NO production in both cell lines, without having cytotoxic effect. Only 4-methylcatechol was cytotoxic at the highest tested concentration (100 µM). Elderberry and elderflower constituents may possess inflammatory modulating activity, which increases their nutritional value.

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Section: Resultssupporting

confidence: 92%

Elderberry and Elderflower Extracts, Phenolic Compounds, and Metabolites and Their Effect on Complement, RAW 264.7 Macrophages and Dendritic Cells

Wangensteen

Barsett

2017

IJMS

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“…Primary antibodies against IjBa, phospho-JNK, JNK, phospho-ERK, ERK, phospho-p38, and p38 (Cell Signaling Technology, Beverly, MA, USA), and ICAM-1, lamin B, and b-actin (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) were supplied commercially. Oligo-(dT) 15 primer was purchased from Promega (Madison, WI, USA). Other oligonucleotide primers (Bioneer, Seoul, Korea) were obtained commercially.…”

Section: Cell Culture and Reagentsmentioning

confidence: 99%

“…Two micrograms of total RNA was reverse transcribed using 10,000 U of reverse transcriptase and 0.5 lg/lL oligo-(dT) 15 primer for 1 h at 37°C. 5,18 The resulting cDNA was amplified by polymerase chain reaction (PCR) using the following sense and antisense primers (5 0 /3 0 ): human ICAM-1sense, CAC CCT AGA GCC AAG GTG AC; human ICAM-1 antisense, CAT TGG AGT CTG CTG GGA AT; human b-actin sense, GCG GGA AAT CGT GCG TGA CAT T; human b-actin antisense, GAT GGA GTT GAA GGT AGT TTC GTG; mouse TNF-a sense, ACA AGC CTG TAG CCC ACG; mouse TNF-a antisense, TCC AAA GTA GAC CTG CCC; mouse IL-1b sense, TGC AGA GTT CCC CAA CTG GTA CAT C; mouse IL-1b antisense, GTG CTG CCT AAT GTC CCC TTG AAT C; mouse IL-6 sense, CAA GAA AGA CAA AGC CAG AGT CCT T; mouse IL-6 antisense, TGG ATG GTC TTG GTC CTT AGC C; mouse b-actin sense, AGT GTG ACG TTG ACA TCC GTA AAG A; and mouse b-actin antisense, GGA CAG TGA GGC CAG GAT GG.…”

Section: Reverse Transcription-polymerase Chain Reaction Analysismentioning

confidence: 99%

“…14 Aronia crude extract exerted protective effects in LPS-stimulated RAW 264.7 cells and endotoxin-induced uveitis in rats by suppressing pro-inflammatory mediators such as iNOS and COX-2. 15,16 Recent studies have shown that A. melanocarpa fruit extract inhibits TNF-a-induced expression of adhesion molecules through suppression of NF-jB activation and reactive oxygen species (ROS) production in human aortic endothelial cells. 12 Although growing evidence suggested various disease-controlling activities of A. melanocarpa fruit, the modulatory activities of A. melanocarpa fruit on skin inflammation and its action mechanisms have not been fully determined.…”

Section: Introduction C Hronic and Dysregulated Inflammatory Responsesmentioning

confidence: 99%

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Aronia melanocarpaConcentrate Ameliorates Pro-Inflammatory Responses in HaCaT Keratinocytes and 12-O-Tetradecanoylphorbol-13-Acetate-Induced Ear Edema in Mice

Goh¹,

Youn

Yoo

et al. 2016

Journal of Medicinal Food

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Abnormal expression of pro-inflammatory mediators such as cell adhesion molecules and cytokines has been implicated in various inflammatory skin diseases, including atopic dermatitis. In this study, we investigated the anti-inflammatory activity of Aronia melanocarpa concentrate (AC) and its action mechanisms using in vivo and in vitro skin inflammation models. Topical application of AC on mouse ears significantly suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema formation, as judged by measuring ear thickness and weight, and histological analysis. Topical administration of AC also reduced the expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in TPA-stimulated mouse ears. Pretreatment with AC suppressed TNF-α-induced ICAM-I expression and subsequent monocyte adhesiveness in human keratinocyte cell line HaCaT. In addition, AC significantly decreased intracellular reactive oxygen species (ROS) generation as well as mitogen-activated protein kinase (MAPK) activation in TNF-α-stimulated HaCaT cells. AC and its constituent cyanidin 3-glucoside also attenuated TNF-α-induced IKK activation, IκB degradation, p65 phosphorylation/nuclear translocation, and p65 DNA binding activity in HaCaT cells. Overall, our results indicate that AC exerts anti-inflammatory activities by inhibiting expression of pro-inflammatory mediators in vitro and in vivo possibly through suppression of ROS-MAPK-NF-κB signaling pathways. Therefore, AC may be developed as a therapeutic agent to treat various inflammatory skin diseases.

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“…이때 세포의 여러 조건에서도 그 발현 정도의 차이 가 거의 없는 house keeping gene인 GAPDH을 positive control로 사용하였다. LPS에 의해 증가된 RAW 264.7 [14,15], 항염증 [16][17][18], 항암 [19], tyrosinase 저해 활성 [20,21], 피부 주름 생성 저해 활성 화시키며 돌연변이 및 DNA 손상을 입힌다 [27][28][29]. …”

Section: Inos 및 Cox-2 Mrna 발현 억제 효과 측정unclassified

Anti-inflammatory Effects of Amelanchier asiatica Fruits Ethanol Extract

Lee¹,

Yoo²,

주다혜³

et al. 2017

Journal of the Society of Cosmetic Scientists of Korea

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This study investigated the anti-inflammatory activities and cell viability of Amelanchier asiatica (A. asiatica) 70% ethanol extracts against RAW 264.7 cells induced by lipopolysaccharide (LPS). Cell toxicity test on macrophage cells (RAW 264.7) was performed by 3-[4,5-dimethyl-thiazol-2-yl]-2, 5-diphenyl-tetrazoliumbromide (MTT) assay and results showed 96% cell viability at 1,000 µg/mL concentration. Anti-inflammatory activity was examined via the inhibitory tests on the production of LPS induced NO in RAW 264.7 cells by Griess assay. The result showed that the extract inhibited NO production in concentration dependent manner. The iNOS and COX-2 protein expression inhibitory effects were confirmed by western blot and by reverse transcription-polymerase chain reaction (RT-PCR). From the former they were decreased by 84.3%, 56.2% at 500 µg/mL concentration, respectively, and from the latter decreased by 89.8%, 84.9% at 500 µg/mL, respectively. In conclusion, this study showed the anti-inflammatory effects of A. asiatica extracts. Thus, this could be applied to an anti-inflammatory agent.

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Immunomodulating Activity of Aronia melanocarpa Polyphenols

Cited by 38 publications

References 27 publications

Elderberry and Elderflower Extracts, Phenolic Compounds, and Metabolites and Their Effect on Complement, RAW 264.7 Macrophages and Dendritic Cells

Elderberry and Elderflower Extracts, Phenolic Compounds, and Metabolites and Their Effect on Complement, RAW 264.7 Macrophages and Dendritic Cells

Aronia melanocarpaConcentrate Ameliorates Pro-Inflammatory Responses in HaCaT Keratinocytes and 12-O-Tetradecanoylphorbol-13-Acetate-Induced Ear Edema in Mice

Anti-inflammatory Effects of Amelanchier asiatica Fruits Ethanol Extract

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