Immunomicroscopical study of type VI collagen in the trabecular meshwork of normal and glaucomatous eyes

Lütjen–Drecoll, Elke; Rittig, Michael; Rauterberg, Jürgen; Jander, Renate; Mollenhauer, Jürgen

doi:10.1016/0014-4835(89)90027-4

Cited by 118 publications

(66 citation statements)

References 23 publications

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“…This network seems to anchor collagen type I/III fibres or the basement membranes of large interstitial structures, such as nerves and blood vessels, into surrounding connective tissue [21]. In accordance with our findings, an increase in type VI collagen has been observed in cerebral blood vessels in arterial hypertension [38], in experiments with haemodynamically stressed vessels [22], in the trabecular meshwork [25] and in the optic nerve [17] of eyes with increased ocular pressure. These findings might indicate that increased stress or strain on connective tissue matrices augments formation of type VI collagen.…”

Section: Discussionsupporting

confidence: 89%

“…We also stained type III collagen, which is regarded as the major component of reticulin fibres in the adventitial dermis [29]. In previous ultraimmunohistochemical studies we were able to demonstrate that in the human eye, broad-banded aggregates similar to zebra bodies or fibrous long-spacing fibres stain with polyclonal antibodies against type VI collagen [25,37]. We therefore studied the localization of type VI collagen in the sheath material of cherry haemangioma capillaries both light-and electron-microscopically.…”

mentioning

confidence: 99%

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Increase in types IV and VI collagen in cherry haemangiomas

Tamm

Jungkunz

et al. 1992

Arch Dermatol Res

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Summary. The capillaries in cherry haemangiomas show perivascular hyalinized sheaths. In order to clarify the nature of this sheath material, the extracellular matrix of cherry haemangiomas from 20 normal volunteers (age range 30-64 years) was investigated using immunohistochemical and electronmicroscopical methods. Antibodies against collagen types III, IV and VI and laminin were used. Hyaluronic acid was visualized using the hyaluronic acid binding region of the cartilage proteoglycan as ligand. Electronmicroscopically, the sheaths contained multilaminated basement membrane-like material, collagen fibres 20-25 nm thick with a periodicity of 67 nm and broadbanded aggregates with a periodicity of 100 nm (zebra bodies or fibrous long-spacing fibres). Immunohistochemically, type IV collagen was stained throughout the whole sheath material. Staining for laminin was more confined to the endothelial side of the sheath. Intense staining for type III collagen and hyaluronic acid was found in the connective tissue of the subpapillary layer and between the cherry haemangioma capillaries. Much weaker staining for type III coUagen and no staining for hyaluronic acid were found invariably in an area 4-10 [tm thick directly around the capillaries. Both sheath material and intercapillary connective tissue of the haemangiomas showed pronounced staining for collagen type VL Immunogold staining revealed that type VI collagen was localized to microfihrils 5-6 nm thick and to the broad-banded aggregates with 100 nm periodicity. These findings further underline the assumption that the broad-banded aggregates consist of type VI collagen.

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Section: Discussionsupporting

confidence: 89%

mentioning

confidence: 99%

Increase in types IV and VI collagen in cherry haemangiomas

Tamm

Jungkunz

et al. 1992

Arch Dermatol Res

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“…They have been observed associated with basement membranes in areas where collagen or elastic fibrils intersect or link to them; associated with the striated collagen fibrils forming a fine filamentous lattice around and between fibrils; associated with the SD microfibrils and to a lesser extent with the dense elastic cores; and associated with long-spaced collagen. (Lütjen-Drecoll et al 1989;Marshall et al 1991;Hernandez and Gong 1996;Lutjen-Drecoll 1999;Ueda et al 2002;Ueda and Yue 2003) Some variations in their precise localizations were observed, probably reflecting differential epitope availability.…”

Section: Collagensmentioning

confidence: 99%

“…Studies with general microscopic stains and electron microscopy identified GAGs, fibrillar collagens, unevenly-spaced or curly collagen, elastic fibrils with associated sheath-derived plaque material, basement membrane and amorphous basement membrane-like materials and other non-specific ECM components. (Hogan et al 1971;Francois 1975;Grierson and Lee 1975;Lütjen-Drecoll et al 1981;Richardson 1982;Lütjen-Drecoll et al 1986;Lütjen-Drecoll and Tamm 1987;Lütjen-Drecoll and Rohen 1996;Lutjen-Drecoll 1999;Gong et al 2002) …”

Section: Ecm Components Identified Within the Tmmentioning

confidence: 99%

“…(Segawa 1979;Lütjen-Drecoll et al 1981;Rohen 1983;Lütjen-Drecoll et al 1986;Lütjen-Drecoll and Tamm 1987;Lutjen-Drecoll and Rohen 2001) The exact molecular nature of this material is not clear and a direct causal link to the increased outflow resistance has been difficult to establish. An additional caveat to these glaucoma studies is that, with few exceptions, (Rohen et al 1993) the tissue is from eyes that have been on various glaucoma medications for extended periods.…”

Section: Glaucoma and Ecmmentioning

confidence: 99%

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Extracellular matrix in the trabecular meshwork

Acott

Kelley

2008

Experimental Eye Research

420

443

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The extracellular matrix (ECM) of the trabecular meshwork (TM) is thought to be important in regulating intraocular pressure (IOP) in both normal and glaucomatous eyes. IOP is regulated primarily by a fluid resistance to aqueous humor outflow. However, neither the exact site nor the identity of the normal resistance to aqueous humor outflow has been established. Whether the site and nature of the increased outflow resistance, which is associated with open-angle glaucoma, is the same or different from the normal resistance is also unclear.The ECMs of the TM beams, juxtacanalicular region (JCT) and Schlemm's canal (SC) inner wall are comprised of fibrillar and non-fibrillar collagens, elastin-containing microfibrils, matricellular and structural organizing proteins, glycosaminoglycans (GAGs) and proteoglycans. Both basement membranes and stromal ECM are present in the TM beams and JCT region. Cell adhesion proteins, cell surface ECM receptors and associated binding proteins are also present in the beams, JCT and SC inner wall region.The outflow pathway ECM is relatively dynamic, undergoing constant turnover and remodeling. Regulated changes in enzymes responsible for ECM degradation and biosynthetic replacement are observed. IOP homeostasis, triggered by pressure changes or mechanical stretching of the TM, appears to involve ECM turnover. Several cytokines, growth factors and drugs, which affect the outflow resistance, change ECM component expression, mRNA alternative splicing, cellular cytoskeletal organization or all of these. Changes in ECM associated with open-angle glaucoma have been identified.

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Pharmacologic Trabeculocanalotomy

Kaufman¹

1992

Arch Ophthalmol

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Immunomicroscopical study of type VI collagen in the trabecular meshwork of normal and glaucomatous eyes

Cited by 118 publications

References 23 publications

Increase in types IV and VI collagen in cherry haemangiomas

Increase in types IV and VI collagen in cherry haemangiomas

Extracellular matrix in the trabecular meshwork

Pharmacologic Trabeculocanalotomy

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