Immunomagnetic separation of circulating tumor cells with microfluidic chips and their clinical applications

Chen, Hongmei; Li, Yong; Zhang, Zhifeng; Wang, Shuangshou

doi:10.1063/5.0005373

Cited by 11 publications

(12 citation statements)

References 134 publications

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“…After magnetization treatment, these CTCs can be attracted to the magnetic field, greatly improving the capture efficiency. [250] Ghassemi et al proposed a microfluidic sensor system that can identify and count CTCs in blood (Figure 3C). CTCs deform as they pass through contractile channels, while blood cells maintain their shape.…”

Section: Deformabilitymentioning

confidence: 99%

Section: Recognition and Capture Of Ctcsmentioning

confidence: 99%

“…In the current recognition and capture techniques for CTCs, some techniques use cell line validation, such as the μ‐LaFF chip the one proposed by Lee et al, the DEP‐FFF device proposed by Gascoyne et al., and the 3D DEP microfluidic platform proposed by Cheng et al., [ 241,259,260 ] using this validation method may not provide sufficiently accurate results. The ISET technology and the hybrid magnet deformable chip used both cell line validation and patient blood samples validation, [ 235a,250 ] which is relatively reliable. Therefore, In the future capture technology of CTCs, it is necessary to conduct both cell line validation and clinical sample validation simultaneously.…”

Section: Recognition and Capture Of Ctcsmentioning

confidence: 99%

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Origin, Metastasis, Harm, Recognition and Capture, and Elimination of Circulating Tumor Cells

Yuan,

Chen,

Wan

et al. 2024

Adv Materials Technologies

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Cancer is now a major threat to human health and life. Although a number of clinical approaches to cancer treatment have been developed in recent years, tumor recurrence and metastasis remain a challenge. As the “seed” for the formation of tumor metastasis, Circulating Tumor Cells (CTCs) can not only grow and spread in the primary site, but also spread to other parts of the body through various ways. The close connection between CTCs and tumor progression makes them a focus of clinical and basic research attention, and it not only has an important guide for the clinical treatment of patients, but also a window into the occurrence and development of distant metastases. The identification and detection of CTCs have an important clinical significance in the assessment of the prognosis of cancer, efficacy prediction, efficacy evaluation, and the study of recurrent metastasis and drug resistance mechanisms of cancer patients. Therefore, this review not only introduces the source, attributes, transfer, and harm of CTCs, but also highlights the progress and latest technologies for the identification and capture and elimination strategies of CTCs and presents prospects for future research of CTCs.

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Section: Deformabilitymentioning

confidence: 99%

Section: Recognition and Capture Of Ctcsmentioning

confidence: 99%

Section: Recognition and Capture Of Ctcsmentioning

confidence: 99%

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Origin, Metastasis, Harm, Recognition and Capture, and Elimination of Circulating Tumor Cells

Yuan,

Chen,

Wan

et al. 2024

Adv Materials Technologies

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“…In the more widespread case of in vitro CTC capture, microfluidics-based approaches offer an attractive alternative to macroscale techniques, such as bulk magnetic sorting, since they are operator-independent and have the potential to provide precise sorting conditions and continuous sample processing, minimizing rare cell loss. Thus, many efforts have been devoted to the development of microfluidic systems based on magnetism to isolate CTCs in the past decade [110,177,[232][233][234], as recently reviewed by Chen et al [235]. The IsoFlux System (Fluxion Biosciences Inc, South San Francisco, CA) is an example of microfluidics-based technology using IMS and dedicated to CTC isolation brought to the market.…”

Section: Microfluidics-based Approachesmentioning

confidence: 99%

Basic Principles and Recent Advances in Magnetic Cell Separation

Frénéa‐Robin

Marchalot

2022

Magnetochemistry

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Magnetic cell separation has become a key methodology for the isolation of target cell populations from biological suspensions, covering a wide spectrum of applications from diagnosis and therapy in biomedicine to environmental applications or fundamental research in biology. There now exists a great variety of commercially available separation instruments and reagents, which has permitted rapid dissemination of the technology. However, there is still an increasing demand for new tools and protocols which provide improved selectivity, yield and sensitivity of the separation process while reducing cost and providing a faster response. This review aims to introduce basic principles of magnetic cell separation for the neophyte, while giving an overview of recent research in the field, from the development of new cell labeling strategies to the design of integrated microfluidic cell sorters and of point-of-care platforms combining cell selection, capture, and downstream detection. Finally, we focus on clinical, industrial and environmental applications where magnetic cell separation strategies are amongst the most promising techniques to address the challenges of isolating rare cells.

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“…As the potential of microfluidics is evident, microfluidic devices are now being commercialized successfully. Commercially available platforms for the detection of CTCs have been reviewed by Chen et al [28] and Habli et al [29]. Further, Sachdeva et al [30] analyzed the commercial landscape of microfluidic POC testing.…”

Section: Physical Measurements In a Microfluidic Hematology Laboratorymentioning

confidence: 99%

Microfluidic Systems for Blood and Blood Cell Characterization

2022

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A laboratory blood test is vital for assessing a patient’s health and disease status. Advances in microfluidic technology have opened the door for on-chip blood analysis. Currently, microfluidic devices can reproduce myriad routine laboratory blood tests. Considerable progress has been made in microfluidic cytometry, blood cell separation, and characterization. Along with the usual clinical parameters, microfluidics makes it possible to determine the physical properties of blood and blood cells. We review recent advances in microfluidic systems for measuring the physical properties and biophysical characteristics of blood and blood cells. Added emphasis is placed on multifunctional platforms that combine several microfluidic technologies for effective cell characterization. The combination of hydrodynamic, optical, electromagnetic, and/or acoustic methods in a microfluidic device facilitates the precise determination of various physical properties of blood and blood cells. We analyzed the physical quantities that are measured by microfluidic devices and the parameters that are determined through these measurements. We discuss unexplored problems and present our perspectives on the long-term challenges and trends associated with the application of microfluidics in clinical laboratories. We expect the characterization of the physical properties of blood and blood cells in a microfluidic environment to be considered a standard blood test in the future.

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Immunomagnetic separation of circulating tumor cells with microfluidic chips and their clinical applications

Cited by 11 publications

References 134 publications

Origin, Metastasis, Harm, Recognition and Capture, and Elimination of Circulating Tumor Cells

Origin, Metastasis, Harm, Recognition and Capture, and Elimination of Circulating Tumor Cells

Basic Principles and Recent Advances in Magnetic Cell Separation

Microfluidic Systems for Blood and Blood Cell Characterization

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