Immunomagnetic separation methods for the isolation of Campylobacter jejuni from ground poultry meats

Yu, Linda; Uknalis, Joseph; Tu, Shu‐I

doi:10.1016/s0022-1759(01)00372-6

Cited by 53 publications

(34 citation statements)

References 20 publications

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“…The two types of microspheres were tested, using three different volumes, and the detection limit of the IMS procedure was approximately 20 UFC/mL for both. This detection limit was similar to that found in other IMS studies with Salmonella (11) and other bacteria such as Actinobacillus pleuropneumoniae and Pasteurella multocida (1) and was better than the 10 3 UFC/mL found for Campylobacter jejuni (17).…”

Section: Resultssupporting

confidence: 88%

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Conceição¹,

Moreira²,

Ramos³

et al. 2008

Braz. J. Microbiol.

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The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S. Enteritidis were analyzed immediately after contamination and after 24h of refrigeration using three enrichment protocols. The detection limit of the IMS-plating procedure after standardization with pure culture was about 2x10 CFU/mL. The protocol using non-selective enrichment for 6-8h, selective enrichment for 16-18h and a post-enrichment for 4h gave the best results of S. Enteritidis detection by IMS-plating in experimentally contaminated meat. IMSplating using this protocol was compared to the standard culture method for salmonellae detection in naturally contaminated chicken cuts and yielded 100% sensitivity and 94% specificity. The method developed using in house prepared magnetic microespheres for IMS and plating in selective agar was able to diminish by at least one day the time required for detection of Salmonella in chicken products by the conventional culture method.

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Section: Resultssupporting

confidence: 88%

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Conceição¹,

Moreira²,

Ramos³

et al. 2008

Braz. J. Microbiol.

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show abstract

“…However, pre-enrichment may still be necessary to obtain a high number of bacteria for detection. Immunocapture followed by plating on selective agar had a threshold of 10 4 CFU per g -1 for the detection of Campylobacter in ground poultry meat (Yu et al, 2001). The sensitivity did not improve when magnetic beads (Dynabeads TM ) were coated with a monoclonal antibody to the major 45 KDa porin and coupled with a DNA hybridization assay specific for the 23S rRNA gene of Campylobacter spp.…”

Section: Concentration By Immunomagnetic Separationmentioning

confidence: 99%

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

Oyarzábal¹,

Battie²

2012

Trends in Immunolabelled and Related Techniques

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“…Specifically, the capture moieties detect the desired target (ie, protein biomarker, DNA, RNA) for subsequent separation from the remaining solution via a simple magnet, and consequently, for various methods of analyses. [2][3][4][5][6] The noninvasiveness and simple nature of this method continues to enable the widespread use of magnetic particles. Furthermore, the high surface-area-to-volume ratio of the particles confers a correspondingly high probability of interaction with target biomarkers, and essentially increases the efficiency of the system.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic nanoparticle-based assays for detection of biomarkers

Park¹,

Hwang²,

Lee³

2013

IJN

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The emergence of biomarkers as key players in the paradigm shift towards preventative medicine underscores the need for their detection and quantification. Advances made in the field of nanotechnology have played a crucial role in achieving these needs, and have contributed to recent advances in the field of medicine. Nanoparticle-based immunomagnetic assays, in particular, offer numerous advantages that utilize the unique physical properties of magnetic nanoparticles. In this review, we focus on recent developments and trends with regards to immunomagnetic assays used for detection of biomarkers. The various immunomagnetic assays are categorized into the following: particle-based multiplexing, signal control, microfluidics, microarray, and automation. Herein, we analyze each category and discuss their advantages and disadvantages.

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Immunomagnetic separation methods for the isolation of Campylobacter jejuni from ground poultry meats

Cited by 53 publications

References 20 publications

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Detection of salmonella sp in chicken cuts using immunomagnetic separation

Immunological Methods for the Detection of Campylobacter spp. - Current Applications and Potential Use in Biosensors

Immunomagnetic nanoparticle-based assays for detection of biomarkers

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