Immunomagnetic reduction assay using high-Tc superconducting-quantum-interference-device-based magnetosusceptometry

Chieh, Jen Jie; Yang, Shieh Yueh; Horng, Herng Er; Yu, Chien-Lin; Lee, C. L.; Wu, Huei-Wen; Hong, Chin Yih; Hong, Yang

doi:10.1063/1.3340861

Cited by 29 publications

(28 citation statements)

References 11 publications

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“…To determine the assay performance of our platform, we applied a logistic fit. 21 According to these results (see Figure 3), we are able to detect sHER2 protein for concentrations 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 10 higher than 0.44 nM (limit of detection, i.e. the sHER2 concentration at which the signal reaches 3x the error of the 1 nM sHER2 sample).…”

Section: Resultsmentioning

confidence: 99%

Homogeneous Protein Analysis by Magnetic Core–Shell Nanorod Probes

Schrittwieser

Pelaz

Parak

et al. 2016

ACS Appl. Mater. Interfaces

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Studying protein interactions is of vital importance both to fundamental biology research and to medical applications. Here, we report on the experimental proof of a universally applicable labelfree homogeneous platform for rapid protein analysis. It is based on optically detecting changes in the rotational dynamics of magnetically agitated core-shell nanorods upon their specific interaction with proteins. By adjusting the excitation frequency, we are able to optimize the measurement signal for each analyte protein size. In addition, due to the locking of the optical signal to the magnetic excitation frequency, background signals are suppressed, thus allowing exclusive studies of processes at the nanoprobe surface only. We study target proteins (soluble domain of the human epidermal growth factor receptor 2 -sHER2) specifically binding to antibodies (trastuzumab) immobilized on the surface of our nanoprobes and demonstrate direct deduction of their respective sizes. Additionally, we examine the dependence of our measurement signal on the concentration of the analyte protein, and deduce a minimally detectable sHER2 concentration of 440 pM. For our homogeneous measurement platform, good dispersion stability of the applied nanoprobes under physiological conditions is of vital importance. To that end, we support our measurement data by theoretical modeling of the total particle-particle interaction energies. The successful implementation of our platform offers scope for applications in biomarker-based diagnostics as well as for answering basic biology questions.

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Section: Resultsmentioning

confidence: 99%

Homogeneous Protein Analysis by Magnetic Core–Shell Nanorod Probes

Schrittwieser

Pelaz

Parak

et al. 2016

ACS Appl. Mater. Interfaces

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“…One of methods for this check is to observe the association between the anti-CEA functionalized Fe 3 O 4 magnetic nanoparticles and CEA molecules in PBS solution. The method used in this experiment for observing the nanoparticle-CEA association is so-called immunomagnetic reduction [24][25][26]. The time dependent ac magnetic susceptibility ac of the reagent mixing with 5 ng/mL CEA solution was recorded and shown with dots in Figure 5.…”

Section: Resultsmentioning

confidence: 99%

“…To check the immunoreactivity of anti-CEA functionalized Fe 3 O 4 magnetic nanoparticles, the technology so-called immunomagnetic reduction is used [24][25][26]. The working principle of immunomagnetic reduction (IMR) is illustrated in Figure 2.…”

Section: Methodsmentioning

confidence: 99%

Time‐Evolution Contrast of Target MRI Using High‐Stability Antibody Functionalized Magnetic Nanoparticles: An Animal Model

Huang

Yang²,

Horng

et al. 2014

Journal of Nanomaterials

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In this work, high-quality antibody functionalized Fe3O4magnetic nanoparticles are synthesized. Such physical characterizations as particle morphology, particle size, stability, and relaxivity of magnetic particles are investigated. The immunoreactivity of biofunctionalized magnetic nanoparticles is examined by utilizing immunomagnetic reduction. The results show that the mean diameter of antibody functionalized magnetic nanoparticles is around 50 nm, and the relaxivity of the magnetic particles is 145 (mM·s)−1. In addition to characterizing the magnetic nanoparticles, the feasibility of using the antibody functionalized magnetic nanoparticles for the contrast medium of target magnetic resonance imaging is investigated. These antibody functionalized magnetic nanoparticles are injected into mice bearing with tumor. The tumor magnetic-resonance image becomes darker after the injection and then recovers 50 hours after the injection. The tumor magnetic-resonance image becomes the darkest at around 20 hours after the injection. Thus, the observing time window for the specific labeling of tumors with antibody functionalized magnetic nanoparticles was found to be 20 hours after injecting biofunctionalized magnetic nanoparticles into mice. The biopsy of tumor is stained after the injection to prove that the long-term darkness of tumor magnetic-resonance image is due to the specific anchoring of antibody functionalized magnetic nanoparticles at tumor.

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“…For more on the status of the perspective biomagnetic high-T c SQUIDs, interested readers may refer to [31]- [34].…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

Low-Noise Serial High- $T_{c}$ $\hbox{YBa}_{2}\hbox{Cu}_{3}\hbox{O}_{y}$ Superconducting Quantum Interference Devices Based on Bicrystal Junctions

Chen

Liao

Chen

et al. 2015

IEEE Trans. Appl. Supercond.

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The fabrication of high-T c YBa 2 Cu 3 O y (YBCO) superconducting quantum interference devices (SQUIDs) that produce low flux noise while maintaining a high fabrication yield continues to challenge scientists. In this paper, we report the characterization of low-noise serial high-T c SQUIDs for biomagnetic applications. Epitaxial high-T c YBCO thin films with a root-mean-square surface roughness less than 5 nm are optimized for the low-noise serial SQUID coupled to a common pickup coil to form magnetometers and gradiometers. Current-voltage curves, voltage-flux curves, and flux noise of SQUID devices are characterized. A smooth surface morphology across the bicrystal line is essential for SQUIDs to show good characteristics. The field sensitivity is improved by a factor of (2) 1/2 with the designed serial SQUID array, which is coupled to a common pickup loop. This is demonstrated, and its fabrication yield is higher than 80% for devices with peak-to-peak voltage V PP ≥ 15 μV. The challenges and perspectives of the high-T c serial SQUID array for biomagnetic applications are addressed and discussed.

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Immunomagnetic reduction assay using high-Tc superconducting-quantum-interference-device-based magnetosusceptometry

Cited by 29 publications

References 11 publications

Homogeneous Protein Analysis by Magnetic Core–Shell Nanorod Probes

Homogeneous Protein Analysis by Magnetic Core–Shell Nanorod Probes

Time‐Evolution Contrast of Target MRI Using High‐Stability Antibody Functionalized Magnetic Nanoparticles: An Animal Model

Low-Noise Serial High- $T_{c}$ $\hbox{YBa}_{2}\hbox{Cu}_{3}\hbox{O}_{y}$ Superconducting Quantum Interference Devices Based on Bicrystal Junctions

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