Immunomagnetic nanoparticle-based sandwich chemiluminescence-ELISA for the enrichment and quantification of E. coli

Pappert, Gerhard; Rieger, Martin; Nießner, Reinhard; Seidel, Michael

doi:10.1007/s00604-009-0264-x

Cited by 60 publications

(32 citation statements)

References 28 publications

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“…Similarly, the USDA microbiology laboratory guidebook (USDA, 2012) suggested methods for Shiga toxin-producing E. coli (STEC) strains detections, which involving both serological tests and polymerase chain reaction (PCR) procedures, could take up to 28 pieces of equipment and materials and may also take one to three days to provide positive identification. Other methods, such as enzyme-linked immunosorbent assay (ELISA) detection for E. coli in water samples (e.g., Pappert et al, 2010), PCR detection for Salmonella spp., Listeria monocytogenes , and Escherichia coli O157:H7 in meat samples (e.g., Kawasaki et al, 2005), and loop-mediated isothermal amplification detection for E. coli strains from various food samples (e.g., Wang et al, 2012), have been used for the detection of foodborne bacteria. While useful, these methods are often coupled to culture enrichment procedures to ensure detection of metabolically-active cells.…”

Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS)

Zhu

Hill

2013

Food Microbiology

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Escherichia coli O157:H7 (EC O157:H7), as well as its recently emerging non-O157 relatives, are a notorious group of pathogenic bacteria associated with foodborne outbreaks. In this study, we demonstrated that secondary electrospray ionization mass spectrometry (SESI-MS) could be a rapid and accurate detection technology for foodborne pathogens. With SESI-MS volatile organic compound (VOC) profiling, we were able to detect and separate a group of eleven E. coli strains from two major foodborne bacteria, S. aureus and S. Typhimurium in three food modeling media. In addition, heat map analysis of relative peak intensity show that there are six core peaks (m/z of 65, 91, 92, 117, 118 and 119) present and at a similar intensity in all eleven E. coli strains at the experimental conditions we tested. These peaks can be considered conserved VOC biomarkers for E. coli species (robustly produced after just four hours of growth). Bacterial strain-level differentiation was also attempted via VOC profiling, and we found that EC O157:H7 and EC O145 were differentiable from all other EC strains under the conditions investigated.

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Section: Introductionmentioning

confidence: 99%

Detection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS)

Zhu

Hill

2013

Food Microbiology

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“…As these holograms can be acquired by cell phone cameras and can also be transferred to a central computer for processing, this device has a potential for pathogen diagnosis in resource-limited settings. In one study, magnetic nanoparticles coupled with antibodies against the enterobacterial common antigen were applied to enrich E. coli cells from 10 mL water samples (Pappert et al 2010 In another study, a simple method was developed to concentrate a model virus in order to reduce the limit of detection of lateral-flow immunoassays (Mashayekhi et al 2010). An aqueous two-phase micellar system was In another study, comparison of the performances of hollow-fiber ultra-filtration and capsule filtration was performed for the concentration of 10 to 1000 Toxoplasma gondii oocysts in 1 L of spiked water, fresh surface water, and sea water samples (Shapiro et al 2010 …”

Section: Immunoassay-based Detection Schemes Amentioning

confidence: 99%

Detection and Occurrence of Indicator Organisms and Pathogens

Seyrig¹,

Herzog²,

Ahmad³

et al. 2011

Water Environment Research

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This review summarizes the studies related to detection and occurrence of fecal indicator bacteria (FIB) and pathogens published during 2010. The first section of this review summarizes various detection methods for waterborne pathogens including endpoint, reverse transcriptase, and real-time polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), microarrays, and immunoassays. Relevant literature on sample processing is also included. The second section focuses on phenotypic and genotypic methods for microbial source tracking. The third section summarizes studies related to occurrence, persistence, and transport with emphasis on microbial quality of recreational beaches and watersheds.

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“…The bioactive coating on the surface captures and magnetically 'tags' the target, enabling magnetic enrichment 8 , washing and resuspension into a clean matrix or buffer to facilitate detection.…”

mentioning

confidence: 99%

Nanoparticle sample preparation and mass spectrometry for rapid diagnosis of microbial infections

2013

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In vitro diagnostics encompasses a wide range of medical devices and assays, which aim to provide reliable and accurate diagnosis of disease. This can be achieved by detecting a target, for example, a protein biomarker or a pathogen bacterium, and/or host factors such as cytokines induced in an inflammatory response. Detection involves an assay to capture target molecules and distinguish them from other substances in an ex vivo sample matrix. Selective capturing can be achieved using affinity probes, such as antibodies or small molecules, often coupled to a label, for example, an enzyme or a particle, to facilitate detection in complex matrixes (Figure 1). Today, the combination of nanoparticle approaches for sample preparation/concentration, with high information content, rapid analysis by mass spectrometry, is changing the way we detect and identify pathogenic bacteria in the diagnosis of microbial infection.Most diagnostic assays are still carried out in centralised laboratories with equipment operated by dedicated laboratory personnel.Decentralised and rapid testing is important for time-critical diagnoses, where time affects morbidity and mortality outcomes for the patient, particularly in the case of bacteria sepsis. The trend towards decentralisation poses demanding constraints on assay technologies. Target molecules are present in body fluids in minute concentrations. To achieve high sensitivity detection, the physics, biology and chemistry of the sensing device, or biosensor, needs to be well integrated and controlled to transduce the presence of specific analytes into a measurable signal. This challenge is even harder when trying to detect bacteria, due to the incredible diversity of the genotype and phenotypes between species and within species. Detection of most pathogens is still carried out by culturing a clinical sample, a process susceptible to contamination, with a long lead time to diagnosis. Magnetic nanoparticles can be decorated with a high density of capture probes due to their extremely high surface-to-volume ratio 7 .Additionally, they can be externally manipulated by controlled magnetic fields in a background of a complex biological matrix.The bioactive coating on the surface captures and magnetically 'tags' the target, enabling magnetic enrichment 8 , washing and resuspension into a clean matrix or buffer to facilitate detection.Sensitive particle-based diagnostic nanotechnologies require efficient suppression of the background arising from non-specific interactions originating from the sample matrix. As a consequence, a great deal of research has focused on the development of robust surface architectures to extend assay applicability to complex matrixes. Hydrophilic polymer coatings (e.g. polyethylene glycol)are extensively exploited to reduce biofouling and to space the bioactive probes from the surface of the particle, which ameliorates risk of steric hindrance for analyte capture 9-11 . Once tagged, the target can be detected magnetically or optically (Table 1)

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Immunomagnetic nanoparticle-based sandwich chemiluminescence-ELISA for the enrichment and quantification of E. coli

Cited by 60 publications

References 28 publications

Detection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS)

Detection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS)

Detection and Occurrence of Indicator Organisms and Pathogens

Nanoparticle sample preparation and mass spectrometry for rapid diagnosis of microbial infections

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