Immunomagnetic microfluidic integrated system for potency-based multiple separation of heterogeneous stem cells with high throughput capabilities

Kang, Byunghoon; Han, Seungmin; Son, Ho‐Young; Mun, Byeonggeol; Shin, Moo-Kwang; Choi, Yuna; Min, Jeong‐Ki; Park, Dae‐Won; Lim, Eun‐Kyung; Huh, Yong Min; Haam, Seungjoo

doi:10.1016/j.bios.2021.113576

Cited by 7 publications

(2 citation statements)

References 58 publications

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“…In MPDA, the hMNPs bound to the PDA/DMPC NPs, so the magnetization value of MPDA decreased slightly compared with that of the hMNPs; however, both hMNPs and MPDA maintained superparamagnetic behavior (Figure 1F). [47] To confirm that MPDA has a colorimetric response, which is a unique characteristic of PCDA, despite the presence of hMNPs, MPDA was polymerized in the same manner as PCDA/DMPC NPs and then stimulated at 70 °C for 30 min, and their absorbance before and after stimulation was measured. The color of MPDA changed upon thermal stimulation (Figure 1G), similar to that of PCDA/DMPC NPs (Figure S1B, Supporting Information).…”

Section: Mpda Preparation and Characteristicsmentioning

confidence: 99%

Colorimetric Detection of HER2‐Overexpressing‐Cancer‐Derived Exosomes in Mouse Urine Using Magnetic‐Polydiacetylene Nanoparticles

Kim,

Mun,

Lim

et al. 2023

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Breast cancer (BC) is a major global health problem, with ≈20–25% of patients overexpressing human epidermal growth factor receptor 2 (HER2), an aggressive marker, yet access to early detection and treatment varies across countries. A low‐cost, equipment‐free, and easy‐to‐use polydiacetylene (PDA)‐based colorimetric sensor is developed for HER2‐overexpressing cancer detection, designed for use in low‐ and middle‐income countries (LMICs). PDA nanoparticles are first prepared through thin‐film hydration. Subsequently, hydrophilic magnetic nanoparticles and HER2 antibodies are sequentially conjugated to them. The synthesized HER2‐MPDA can be concentrated and separated by a magnetic field while inheriting the optical characteristics of PDA. The specific binding of HER2 antibody in HER2‐MPDA to HER2 receptor in HER2‐overexpressing exosomes causes a blue‐to‐red color change by altering the molecular structure of the PDA backbone. This colorimetric sensor can simultaneously separate and detect HER2‐overexpressing exosomes. HER2‐MPDA can detect HER2‐overexpressing exosomes in the culture medium of HER2‐overexpressing BC cells and in mouse urine samples from a HER2‐overexpressing BC mouse model. It can selectively isolate and detect only HER2‐overexpressing exosomes through magnetic separation, and its detection limit is found to be 8.5 × 108 particles mL−1. This colorimetric sensor can be used for point‐of‐care diagnosis of HER2‐overexpressing BC in LMICs.

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Section: Mpda Preparation and Characteristicsmentioning

confidence: 99%

Colorimetric Detection of HER2‐Overexpressing‐Cancer‐Derived Exosomes in Mouse Urine Using Magnetic‐Polydiacetylene Nanoparticles

Kim,

Mun,

Lim

et al. 2023

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“…Magnetic nano- and microparticles can carry various analytes such as proteins, nucleic acids, and cells for medical diagnosis under the control of a magnetic field. − Approaches based on magnetic particle detection are projected to account for over 58% of the $141.65 billion in vitro diagnostics market by 2030. Typically magnetic particles, commonly called magnetic beads, consist of a ferrite core and a polymer or silica shell surface-modified with molecules or antibodies for biological recognition and specific reaction. , The manipulation of such magnetic beads is typically realized by permanent magnets that are either manually controlled or automatically operated to achieve particle separation and reaction product collection. ,− As complex mechanical structures are typically required to physically rotate and translate the magnet, a permanent magnet-based system is difficult to miniaturize for portable analysis.…”

Section: Introductionmentioning

confidence: 99%

Fully Integrated Microfluidic Device for Magnetic Bead Manipulation to Assist Rapid Reaction and Cleaning

Yang,

Ren,

et al. 2023

Anal. Chem.

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Methods to manipulate magnetic beads are essential factors to determine the efficiency and dimension of an in vitro diagnostic system. Currently, using movable permanent magnets and planar electromagnets is still the major approach to achieve magnetic bead control, causing significant constraint in the miniaturization of in vitro diagnostic systems. Here, we propose techniques to construct a fully integrated microfluidic device that can conduct automatic magnetic bead manipulation as well as rapid chemical reaction and cleaning in a minimized dimension similar to a USB disk. The device combines the precision control of multiple electromagnetic coils with the compactness of microfluidic channels, leading to one of the smallest automatic magnetic bead manipulation systems that can complete several major magnetic bead-based operation steps such as sample injection, reaction, cleaning, and collection. The influencing factors such as coil driving parameters, surface treatment of the microchannels, and properties of magnetic particles have also been investigated to optimize the device performance. The device can drive mixtures of Fe3O4 microparticles and polymer magnetic beads (PMBs) with a weight ratio of 1:1 at a maximum speed of 0.5 cm·s–1 and reduce the time for DNA binding and dissociation reactions from 20 min to only 48 s. This device has significantly advanced the conventional manipulation methods of magnetic beads and has demonstrated the possibility to construct an automatic and ultraminiaturized in vitro diagnostic system that may facilitate portable or even wearable chemical analysis.

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Magnetic frequency mixing technological advances for the practical improvement of point‐of‐care testing

Wang

Zheng

et al. 2021

Biotech & Bioengineering

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Nanomaterials, especially superparamagnetic nanomaterials, have recently played essential roles in point-of-care testing due to their intrinsic magnetic, electrochemical, and optical properties. The inherent superparamagnetism of magnetic nanoparticles makes them highly sensitive for quantitative detection. Among the various magnetic detection technologies, frequency mixing technology (FMT) technology is an emerging detection technique in the nanomedical field. FMT sensors have high potential for development in the field of biomedical quantitative detection due to their simple structure, and they are not limited to the materials used. In particular, they can be applied for large-scale disease screening, early tumor marker detection, and low-dose drug detection. This review summarizes the principles of FMT and recent advances in the fields of immunoadsorption, lateral flow assay detection, magnetic imaging, and magnetic nanoparticles recognition. The advantages and limitations of FMT sensors for robust, ultrasensitive biosensing are highlighted. Finally, the future requirements and challenges in the development of this technology are described. This review provides further insights for researchers to inspire the future development of FMT by integration into biosensing and devices with a broad field of applications in analytical sensing and clinical usage.frequency mixing technology (FMT), lateral flow assay (LFA), magnetic imaging, magnetic nanoparticles (MNPs), point-of-care testing (POCT) | INTRODUCTIONRapid medical diagnosis is difficult using current laboratory testing techniques and their application is hindered by factors such as equipment requirements, operator capabilities, and the testing time.In particular, there is an increasing demand for rapid, convenient, and high precision medical diagnosis techniques for detecting coronavirus disease 2019 (COVID-19), severe acute respiratory syndrome (SARS), and other highly contagious infectious diseases (Alfadly et al., 2020;Chu et al., 2021;X. Dong et al., 2021). Point-of-care testing (POCT) can be used for in vitro clinical testing and diagnosis (Wei et al., 2021;Yan et al., 2019). POCT usually refers to detection techniques that do not require a professional operator to perform tests at the bedside next to the patient (Nichols, 2003;Wang et al., 2021;Xia et al., 2016). POCT is characterized by rapid analysis at the sampling site, and thus complicated laboratory processing procedures are not required (Sharma et al., 2021). POCT methods might not have the same level of sensitivity and accuracy compared with laboratory-level medical diagnosis techniques. However, with the advantages of its simple implementation and lack of requirement for professional operational support, this technology receives widespread attention in countries and regions, especially those with a low level of

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Immunomagnetic microfluidic integrated system for potency-based multiple separation of heterogeneous stem cells with high throughput capabilities

Cited by 7 publications

References 58 publications

Colorimetric Detection of HER2‐Overexpressing‐Cancer‐Derived Exosomes in Mouse Urine Using Magnetic‐Polydiacetylene Nanoparticles

Colorimetric Detection of HER2‐Overexpressing‐Cancer‐Derived Exosomes in Mouse Urine Using Magnetic‐Polydiacetylene Nanoparticles

Fully Integrated Microfluidic Device for Magnetic Bead Manipulation to Assist Rapid Reaction and Cleaning

Magnetic frequency mixing technological advances for the practical improvement of point‐of‐care testing

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