Immunomagnetic isolation of magnetoferritin‐labeled cells in a modified ferrograph

Abstract

Pan T, helper, and cytotoxic lymphocytes were isolated from the human peripheral blood mononuclear cell fraction by antibody staining, ferritin labeling, and deposition on glass slides. Two distinct forms of ferritin were used: one was native horse spleen ferritin, and the other was magnetoferritin. Magnetoferritin was obtained by reconstituting the horse spleen ferritin iron core with superparamagnetic magnetite instead of the usual paramagnetic ferrihydrite crystal. The cell deposition on microscopic glass slides in the magnetic field was obtained by an instrument that was adapted from an industrial magnetic deposition analyzer, the ferrograph. The identity of cells in the magnetic deposits was confirmed by comparing the cell fractions in the feed and in the eluate with the use of flow cytometry. The immunostaining protocol amplified the number of ferritin molecules per cell surface antigen 20-70 times. Magnetoferritin, but not native ferritin, imparted a sufficient magnetic moment to cells to deplete the labeled cell population between 67 and 88% of its initial concentration in a magnetic field of 1.67 Tesla (T), a field gradient of 2.57 T/mm, and a flow rate of 0.01 ml/min. This study showed that the magnetic moment of magnetoferritin was sufficient for immunomagnetic isolation of lymphocytes from mononuclear cell preparations in the modified ferrograph.

Aqueous Near‐Infrared Fluorescent Composites Based on Apoferritin‐Encapsulated PbS Quantum Dots

“…[271] They used magnetoferritin for immunogenetic isolation of lymphocytes from mononuclear cell samples. Magnetoferritin was synthesized as described previously.…”

Diagnosis of Malaria by Magnetic Deposition Microscopy

“…[15][16][17][18][19] The magnetic field was designed to maximize local Maxwell stress gradients that drive cell separation from the flowing suspension ( Figure 1A), optimizing erythrocyte capture from the cell suspension. 20 A fringing field of an interpolar gap, combined with a thin flow channel pressed against the interpolar gap, was used as a means of cell capture from suspension.…”

Magnetic isolation of particles suspended in synovial fluid for diagnostics of natural joint chondropathies

“…So far, BF has been used to track Escherichia coli bacteria at low concentrations in natural waters [28][29][30], characterize biofilms within ships' water ballast tanks [31], separate breast cancer and other cells from human peripheral blood [32,33], separate polyethylene wear debris from hip simulator fluid [34], capture of carbon nanoparticles produced by a pulsed arc submerged in ethanol [27] and determination of the role of magnetic minerals embedded in the comb cells of Vespinae [35].…”