Immunology of skin transplantation

Richters, C. D.; Hoekstra, M.J.; Pont, J.S. du; Kreis, R.W.; Kamperdijk, E. W. A.

doi:10.1016/j.clindermatol.2004.07.022

Cited by 55 publications

(47 citation statements)

References 26 publications

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“…Treatment with NaOH for 2 or 4 weeks was apparently too short to remove all antigenic components; an inflammatory response was induced that most probably interfered with the outgrowth of the epidermis from the autologous skin graft. We have observed earlier in other experiments that inflammatory cells can slow down keratinocyte outgrowth; in a rat partial excision wound model (Richters et al 2004) but also in full thickness wound in a porcine model (Richters et al 2005). These wounds were grafted with the sandwich technique; meshed autologous skin covered with fresh allogeneic skin or glycerol preserved allogeneic skin.…”

Section: Discussionmentioning

confidence: 99%

Development of a dermal matrix from glycerol preserved allogeneic skin

et al. 2008

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Dermal substitutes can be used to improve the wound healing of deep burns when placed underneath expanded, thin autologous skin grafts. Such dermal matrix material can be derived from xenogeneic or human tissue. Antigenic structures, such as cells and hairs must be removed to avoid adverse inflammatory response after implantation. In this study, a cost-effective method using low concentrations of NaOH for the de-cellularization of human donor skin preserved in 85% glycerol is described. The donor skin was incubated into NaOH for different time periods; 2, 4, 6 or 8 weeks. These dermal matrix prototypes were analyzed using standard histology techniques. Functional tests were performed in a rat subcutaneous implant model and in a porcine transplantation model; the prototypes were placed in full thickness excision wounds covered with autologous skin grafts.An incubation period of 6 weeks was most optimal, longer periods caused damage to the collagen fibers. Elastin fibers were well preserved. All prototypes showed intact biocompatibility in the rat model by the presence of ingrowing blood vessels and fibroblasts at 4 weeks after implantation. An inflammatory response was observed in the prototypes that were treated for only 2 or 4 weeks with NaOH. The prototypes treated with 6 or 8 weeks NaOH were capable to reduce wound contraction in the porcine model. In neo-dermis of these wounds, elastin fibers derived from the prototype could be observed at 8 weeks after operation, surrounded by more random orientated collagen fibers. Thus, using this effective low cost method, a dermal matrix can be obtained from human donor skin. Further clinical studies will be performed to test this material for dermal substitution in deep (burn) wounds.

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Section: Discussionmentioning

confidence: 99%

Development of a dermal matrix from glycerol preserved allogeneic skin

et al. 2008

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“…It is also known that tissue injury trigger immunosuppressive factors such as proinflammatory cytokines, prostaglandin E2 and stress hormones resulting in the depression of T cell numbers (Schaffer and Barbul, 1998). Furthermore, Richters et al (2005) reported that CD4 + or CD8 + T cell did not migrate into the graft after the skin auto-grafting. Taken together, reduction of CD4 + T cell numbers at day 3 in syngeneic group is considered due to the influence of scale graft operation.…”

Section: Discussionmentioning

confidence: 97%

Kinetics of lymphocyte subpopulations in allogeneic grafted scales of ginbuna crucian carp

Shibasaki

Matsuura

Toda

et al. 2015

Developmental & Comparative Immunology

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“…However, allogeneic skin grafts evoke potent immune responses. Following the transplantation of skin onto a healthy MHC-mismatched recipient, a quick immune rejection process will lead to the failure of the graft within 10-14 days (Richters et al 2005) with characteristic skin tissue destruction. Allogeneic skin grafts are rejected in a short time period of approximately 20-30 days, even if grafts are placed over the fully excised burn wound base (Bravo et al 2000;Hansbrough et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Fundamental Immunology of Skin Transplantation and Key Strategies for Tolerance Induction

Zhou

Wang

Luo

et al. 2013

Arch. Immunol. Ther. Exp.

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Transplantation of allogeneic or xenogeneic skin grafts can evoke strong immune responses that lead to acute rejection of the graft tissues. In this process, donor-derived dendritic cells play crucial roles in the triggering of such immune responses. Both the innate and acquired host immune systems participate in graft rejection. At present, the rejection of skin grafts cannot be well-controlled by ordinary systemic immunosuppression therapy. Although several strategies for the long-term survival of allogeneic or xenogeneic skin grafts have been demonstrated in animal models, the induction of long-term tolerance to skin grafts is still a great challenge in clinical settings. In this article, we review the progress in the understanding of immune responses to skin grafts and discuss the possible methods that can decrease the immunogenicity of graft tissues and improve the survival of skin grafts, especially those included in preoperative pre-treatments.

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Immunology of skin transplantation

Cited by 55 publications

References 26 publications

Development of a dermal matrix from glycerol preserved allogeneic skin

Development of a dermal matrix from glycerol preserved allogeneic skin

Kinetics of lymphocyte subpopulations in allogeneic grafted scales of ginbuna crucian carp

Fundamental Immunology of Skin Transplantation and Key Strategies for Tolerance Induction

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