Immunological substance testing on human lymphatic micro-organoids in vitro

In vitro innate immune cell based models to assess whole cell Bordetella pertussis vaccine quality: A proof of principle

“…Functional immunological assays could complement this approach and ultimately reduce animal based lot release testing. Although previous studies have shown that APC platforms can be used in vitro to determine vaccineinduced responses in R&D [26,27], this is the first evaluation of these types of APC platforms for monitoring vaccine quality. Here, we provide proof-of-principle that moDC and MM6 cells can be used to monitor the quality of wP vaccine in vitro, although validation experiments will be necessary.…”

Substrate-bound CCL21 and ICAM1 combined with soluble IL-6 collectively augment the expansion of antigen-specific murine CD4+ T cells

“…[8][9][10][11][12][13][14][15][16][17][18][19][20][21] The development of SINs for the selective stimulation of specific T cells is a particularly challenging mission, as it must encompass the broad diversity of natural immune niches and the complex interplay between the stromal and immune cell types that reside within them. These studies have provided valuable insights into the molecular complexity and specific functionalities of the various factors residing in natural immune niches, but have yielded limited information on the synergy between them, nor have they addressed the role of topology in their effective integration.…”

Biomaterial Strategies for Immunomodulation

“…Giese et al (146, 147) employed agarose gels or nonwoven polyamide fiber scaffolds as matrices for human T and B cells, and DCs that mimic the extremely high cell densities present in lymphoid tissues. These constructs have shown the ability to support antibody responses to licensed vaccines and physiologically relevant alterations in cytokine production in response to immunosuppressive drugs (146).…”

“…Giese et al (146, 147) employed agarose gels or nonwoven polyamide fiber scaffolds as matrices for human T and B cells, and DCs that mimic the extremely high cell densities present in lymphoid tissues. These constructs have shown the ability to support antibody responses to licensed vaccines and physiologically relevant alterations in cytokine production in response to immunosuppressive drugs (146). Higbee et al (148) developed a modular in vitro system termed MIMIC (Modular Immune In vitro Construct) with separate in vitro scaffolds representing peripheral tissues and LNs, and that aimed to predict the responses of cultured human leukocytes to vaccine administration or other biologicals.…”