Immunological Study on Marginal Periodontitis. 1

Abstract

Twenty pregnant women with marginal periodontitis and 8 pregnant women without periodontitis, aged between 21 and 30 years old, were examined. The stage of periodontitis was classified according to the slight modification of the periodontal index of Russel. Serum protein fractions were separated by electrophoresis on cellulose acetate membrane, using veronal buffer, pH 8.6, ionic strength 0.06, and measured by a densitometer after staining with Ponceau 3R. For the immuno-electrophoretic analysis agar gel plates were used. Buffer solution was veronal buffer, pH 8.6, ionic strength 0.025. Anti-human serum rabbit sera were prepared in our laboratory.