Immunological Studies on the Occurrence and Properties of Chromogranin A and B and Secretogranin II in Endocrine Tumors

Weiler, Rosa Maria Eid; Fischer‐Colbrie, Reiner; Schmid, Kurt Werner; Feichtinger, Hans G.; Bussolati, Gianni; Grimelius, Lars; Krisch, K; Kerl, Helmut; O'Connor, D.; Winkler, Hans

doi:10.1097/00000478-198811000-00008

Cited by 101 publications

(37 citation statements)

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“…For immunoblotting with a polyclonal antiserum [14] against human chromogranin (dilution 1:200) heat stable extracts (corresponding to 1 g wet tissue) of lyophilized brain tissue [15] were used. For synaptin/synaptopbysin (polyclonal antiserum against rat synaptin/synaptophysin kindly provided by R. Jahn, Munich, FRG) lyophilized tissue samples (corresponding to 25 mg wet tissue) were dissolved directly in electrophoresis sample buffer.…”

Section: Methodsmentioning

confidence: 99%

A high ratio of chromogranin A to synaptin/synaptophysin is a common feature of brains in Alzheimer and Pick disease

et al. 1990

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Chromogranin A and synaptin/synaptophysin were characterized by immunological methods in human autopsy brain tissue from patients with Alzheimer's and Pick's disease. In immunoblots there was no qualitative difference between the antigens in control and diseased brain, but significant quantitative differences were found. In all Alzheimer cases there was a significantly lower level of synaptin/synaptophysin, whereas chromogranin A was higher in 4 out of 5 cases and in all cases relative to synaptin/synaptophysin. An analogous findingwas obtained for Pick's disease. Immunohistologically a consistent staining of neuritic plaques for chromogranin A, but not for secretogranin II was found in Alzheimer cases. In Pick's disease the characteristic Pick bodies showed an analogous specific immunostaining.

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Section: Methodsmentioning

confidence: 99%

A high ratio of chromogranin A to synaptin/synaptophysin is a common feature of brains in Alzheimer and Pick disease

et al. 1990

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“…To validate the differential expression of the proteins detected by 2-DGE proteomic pattern comparison by western blot we selected chromogranin B, as its expression had been documented in pheochromocytoma in prior studies, but with no consideration of the patient's genetic background (Lloyd et al 1988, Weiler et al 1988, Portela-Gomes et al 2004. Tissue samples from five MEN2A-and five VHL-associated pheochromocytomas were investigated for expression of chromogranin B.…”

Section: Validation Of Differentially Expressed Proteinmentioning

confidence: 99%

“…VHL-associated pheochromocytoma may lack the machinery for catecholamine storage Among the differentially expressed proteins identified, we selected chromogranin B for further validation as its expression has been studied in pheochromocytoma previously (Lloyd et al 1988, Weiler et al 1988, Portela-Gomes et al 2004). However, these prior studies did not investigate pheochromocytomas from different genetic backgrounds.…”

Section: F M Brouwers Et Al: Comparison Of Vhl and Men2a Pheochromocmentioning

confidence: 99%

Proteomic profiling of von Hippel–Lindau syndrome and multiple endocrine neoplasia type 2 pheochromocytomas reveals different expression of chromogranin B

Brouwers

Gläsker

Nave³

et al. 2007

Endocr Relat Cancer

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Pheochromocytomas are catecholamine-producing tumors that can occur in the context of von Hippel-Lindau syndrome (VHL) and multiple endocrine neoplasia type 2 (MEN2). Pheochromocytomas in these two syndromes differ in histopathological features, catecholamine metabolism, and clinical phenotype. To further investigate the nature of these differences, we compared the global protein expressions of 8 MEN2A-associated pheochromocytomas with 11 VHL-associated pheochromocytomas by two-dimensional gel electrophoresis proteomic profiling followed by sequencing and identification of differentially expressed proteins. Although both types of pheochromocytoma shared similarities in their protein expression patterns, the expression of several proteins was distinctly different between VHL-and MEN2A-associated pheochromocytomas. We identified several of these differentially expressed proteins. One of the proteins with higher expression in MEN2-associated tumors was chromogranin B, of which the differential expression was confirmed by western blot analysis. Our results expand the evidence for proteomic differences between these two tumor entities, and suggest that VHL-associated pheochromocytomas may be deficient in fundamental machinery for catecholamine storage. In light of these new findings, as well as existing evidence for differences between both types of pheochromocytomas, we propose that these tumors may have different developmental origins.

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“…Perhaps the most widely expressed such protein, especially in neuroendocrine neoplasia (15), is chromogranin A. We therefore analyzed its promoter region for clues to mechanisms universally governing gene expression in the neuroendocrine system, that is, elements controlling acquisition of the neuroendocrine secretory phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Among the chromogranins/secretogranins, perhaps the most widespread in distribution, especially in neuroendocrine neoplasia (15), is chromogranin A, a 48-kD protein encoded by a recently isolated (6, 7) eight-exon gene whose chromosomal position has been established in homologous (conserved) regions in man (16,17), mouse (18), rat (18), and cattle (19).…”

Section: Introductionmentioning

confidence: 99%

Cell type-specific gene expression in the neuroendocrine system. A neuroendocrine-specific regulatory element in the promoter of chromogranin A, a ubiquitous secretory granule core protein.

Wu¹,

Rozansky²,

Webster³

et al. 1994

J. Clin. Invest.

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Immunological Studies on the Occurrence and Properties of Chromogranin A and B and Secretogranin II in Endocrine Tumors

Cited by 101 publications

References 0 publications

A high ratio of chromogranin A to synaptin/synaptophysin is a common feature of brains in Alzheimer and Pick disease

A high ratio of chromogranin A to synaptin/synaptophysin is a common feature of brains in Alzheimer and Pick disease

Proteomic profiling of von Hippel–Lindau syndrome and multiple endocrine neoplasia type 2 pheochromocytomas reveals different expression of chromogranin B

Cell type-specific gene expression in the neuroendocrine system. A neuroendocrine-specific regulatory element in the promoter of chromogranin A, a ubiquitous secretory granule core protein.

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