Immunological responses to antigen B from <i>Echinococcus granulosus</i> cyst fluid in hydatid patients

Ioppolo, S.; Notargiacomo, S; Profumo, Elisabetta; Franchi, Cristiana; Ortona, Elena; Riganò, Rachele; Siracusano, Alessandra

doi:10.1046/j.1365-3024.1996.d01-31.x

Cited by 65 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An enzyme-linked immunosorbent assay or indirect haemagglutination test is commonly used as an initial screen, and is positive in onlyy50% of patients with pulmonary hydatidosis and w90% of patients with hepatic cysts [38]. Testing for specific antibodies, such as specific immunoglobulin G1 or G4 rather than total immunoglobulin G, may improve the specificity of a test [39,40]. The specificity of these tests increases considerably when utilising antigen 5 (arc 5 test), immunoblotting or gel diffusion assay for specific hydatid antigen.…”

Section: Laboratory and Special Investigationsmentioning

confidence: 99%

Pulmonary echinococcosis

Morar¹,

Feldman²

2003

Eur Respir J

271

317

View full text Add to dashboard Cite

Section: Laboratory and Special Investigationsmentioning

confidence: 99%

Pulmonary echinococcosis

Morar¹,

Feldman²

2003

Eur Respir J

271

317

View full text Add to dashboard Cite

“…2,7,9 Both antigens proved to exhibit good diagnostic parameters, although a variable range of diagnostic sensitivities and specificities have been documented by different groups. 6,7,[10][11][12][13][14] One of the major obstacles encountered in the evaluation of these serologic procedures was the lack of definition of the diagnostic status of the sera used and the lack of performance under real routine diagnostic conditions. Thus, only a few studies have included malignancies as one of the most common pathologies to be taken into account in the differential diagnosis of space-occupying lesions.…”

mentioning

confidence: 99%

Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies.

Poretti

Felleisen²,

Grimm³

et al. 1999

The American Journal of Tropical Medicine and Hygiene

109

View full text Add to dashboard Cite

Abstract.We assessed an Echinococcus granulosus hydatid fluid antigen-ELISA (EgHF-ELISA) as a serologic prescreening test for E. granulosus infections, supplemented by more specific confirmatory tests, including arc-5 immunoprecipitation and antigen B subunit 8-kD immunoblotting. The diagnostic sensitivity of the EgHF-ELISA was 91%. With regard to the test specificity of the EgHF-ELISA (overall ϭ 82%), we observed relatively frequent crossreactions in tumor patients (6%) and in patients with other parasitic diseases. Cestode-related cross-reactivity can be resolved by the complementary use of E. multilocularis-specific antigens or Taenia solium cysticercosis-specific immunoblotting. Immunoblotting based upon the detection of antibody reactivity to the 8-kD antigen of EgHF, or if appropriately detectable, to the 29-kD and 34-kD bands exhibited a 91% diagnostic sensitivity and an overall specificity of 97% or 94%, respectively. Thus, immunoblotting provided a 99% discrimination between seropositive preoperative cystic hydatid disease cases and cross-reactive non-cestode parasitic infections or malignancies.For primary serologic diagnosis and for support of clinical diagnosis of cystic echinococcosis (cystic hydatid disease [CHD]), the selection of a particular immunodiagnostic test involves consideration of the diagnostic operating characteristics of the technique and the purpose for which it will be used. The diagnostic sensitivity and specificity of the tests vary according to the nature and quality of the antigen and the methodologic sensitivity of the selected technology. A concise definition of the sera used for the assessment of test parameters is essential, with special attention paid to the definition of pre-or post-operative situations respective to CHD serum sampling time point. One of the most specific conventional immunodiagnostic approaches for CHD relies upon the demonstration of serum antibodies precipitating an antigen called antigen 5 by immunoelectrophoresis or similar techniques.1 Diagnostic sensitivities with respect to hepatic CHD have been reported to vary between 50% and 80%. Antibodies to antigen 5 also occurred in serum of human patients with neurocysticercosis 3 and alveolar echinococcosis (AE). 4 Comparative studies showed that 58% of Swiss patients with AE were arc 5-positive compared with 74% of patients with CHD. 5 Resolution of E. granulosus hydatid cyst fluid by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by immunoblotting, resulted in the identification of the arc-5 subunits, including two subunits with relative molecular masses estimated by different laboratories to be between 37 and 38 kD and 20-22-24 kD, respectively. 2 Diagnostic assessment of these two antigens by immunoblotting performed in different laboratories have resulted in the publication of discrepant sensitivity and specificity parameters. 2,6,7 The second major parasite antigen in hydatid cyst fluid is a thermostable lipoprotein called antigen B. The major components of antigen B r...

show abstract

“…Because of the association-specific response to antigens of E. granulosus, it can reduce cross-reactivity, whereas IgG4 recognizes antigen B or other immunomodulatory molecules; therefore, it would be a choice for serodiagnostic tests of complementary value associated with a total IgG assay. 12,[20][21][22][23][24] rEPC1 has a relatively promising performance in total IgG ELISA for the detection of antibodies in sera from patients with CE.…”

Section: Discussionmentioning

confidence: 99%

A Study of Cross-Reactivity Between Recombinant EPC1 Antigen of Echinococcus granulosus in Serum from Patients with Confirmed Cystic Echinococcosis Infection and Other Parasitic Infections

Fathi¹,

Jalousian²,

Hosseini³

et al. 2016

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. A standardized test for the serodiagnosis of cystic echinococcosis (CE) remains an important challenge because of the problems in specificity and sensitivity of the available commercial kits and lack of proper evaluation of antigen. Using appropriate sources of antigenic material is crucial in improvement of the serological methods such as enzyme-linked immunosorbent assay (ELISA). This study was conducted to evaluate the performance of protein named Echinococcus protoscolex calcium binding protein EPC1 for the detection of antibodies in sera from patients with CE. Expressed and purified recombinant protein EPC1 (rEPC1) was used as antigen in ELISA method. Characterization of the rEPC1 antigen was evaluated using the serum of 25 patients with both surgical and imaging confirmed CE and 25 healthy donors as negative controls. Also, a panel of sera including chronic toxoplasmosis (IgG positive), strongyloidosis, fascioliasis, toxocariasis, and kala azar were used and patients with related parasites were confirmed by medical laboratories or clinically by research centers using microscopy or specific ELISA. rEPC1 showed relatively promising performance in total IgG ELISA for the detection of antibodies in sera from the negative controls, and the cut off value 0.4 units of optical density at 490 nm was calculated for ELISA. In this study, sensitivity of 100%, specificity of 93.7, positive predictive value of 92.6%, and negative predictive value of 100% were calculated for rEPC1. On the other hand, commercial ELISA kit based on the native antigen B of Echinococcus granulosus had sensitivity of 96.2% and specificity of 96.8%. No significant difference was found for sensitivity or specificity between the rEPC1 and commercial kit. However, rEPC1 may be a valuable antigen for diagnosis of human CE.

show abstract

Immunological responses to antigen B from Echinococcus granulosus cyst fluid in hydatid patients

Cited by 65 publications

References 0 publications

Pulmonary echinococcosis

Pulmonary echinococcosis

Differential immunodiagnosis between cystic hydatid disease and other cross-reactive pathologies.

A Study of Cross-Reactivity Between Recombinant EPC1 Antigen of Echinococcus granulosus in Serum from Patients with Confirmed Cystic Echinococcosis Infection and Other Parasitic Infections

Contact Info

Product

Resources

About