Immunological Response against Allogeneic Chondrocytes Transplanted into Joint Surface Defects in Rats

Hyc, Anna; Malejczyk, Jacek; Osiecka, Anna; Moskalewski, Stanisław

doi:10.1177/096368979700600205

Cited by 11 publications

(10 citation statements)

References 21 publications

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“…in sera of intact animals and recipients of syngeneic transplants, like in the previous studies (17,33). Cyto toxic activity was significantly increased in sera of un protected allogeneic transplant recipients and remained at the control level in animals with protected transplants (Fig.…”

Section: Cytotoxic Activity Against Chondrocytes Was Presentsupporting

confidence: 81%

“…Cartilage formed by isolated syngeneic (Fig. la) and allogeneic chondrocytes in unprotected transplants filled the defect and had similar microscopic appearance as described previously (17,30,33). Superficial chondro cytes were flattened, like in intact articular cartilage (Fig.…”

Section: Unprotected Chondrocyte Transplantssupporting

confidence: 64%

“…Like in the previous reports (17,33), SMC from recip ients of unprotected allogeneic transplants were stimu lated by allogeneic chondrocytes. The response was con siderably stronger after 3 weeks than after 8 weeks posttransplantation.…”

Section: Evaluation Of Antichondrocyte Immune Responsementioning

confidence: 80%

“…Thus, the use of allogeneic chondrocytes for cartilage repair would be of considerable value. However, cartilage produced by al logeneic chondrocytes in articular surface defects in rats has been resorbed by infiltrating immune cells (17) and its rejection was not prevented even by strong immuno suppressive agents (33). Nevertheless, these experiments suggested another approach aimed at the protection of recipients against sensitization by transplanted chondro cytes.…”

Section: Introductionmentioning

confidence: 99%

“…The ability of syngeneic and allogeneic chondrocytes to stimulate SMC was evaluated as described previously(17,33). In brief, splenocytes were mixed with chondro cytes in wells of culture plates and cultured for 96 h.…”

mentioning

confidence: 99%

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Mechanical Barrier as a Protection against Rejection of Allogeneic Cartilage Formed in Joint Surface Defects in Rats

et al. 2000

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Cartilage formed in transplants of allogeneic chondrocytes into joint cartilage defects in rats was infiltrated by immune cells migrating from the bone marrow while the surface on the side of the joint cavity remained free of infiltrations. This suggested that immunization occurred via bone marrow and not via joint cavity. Because articular cartilage is nourished exclusively by the synovial fluid, we have attempted to prevent cartilage rejection by protecting transplants from the contact with bone marrow. Defects in articular surface were filled with bone cement and chondrocytes were transplanted into a cavity prepared within the bone cement plug. Cartilage formed within the cement shell remained free of infiltrations and did not evoke systemic immunological response. However, distribution of glycosaminoglycans in the matrix of protected transplants was irregular. Cultures of chondrocytes growing in vitro on cement contained less glycosaminoglycans than the controls. This suggests that some factor(s) released from the cement unfavorably influenced chondrocytes and matrix production in protected transplants.

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Section: Cytotoxic Activity Against Chondrocytes Was Presentsupporting

confidence: 81%

Section: Unprotected Chondrocyte Transplantssupporting

confidence: 64%

Section: Evaluation Of Antichondrocyte Immune Responsementioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Mechanical Barrier as a Protection against Rejection of Allogeneic Cartilage Formed in Joint Surface Defects in Rats

et al. 2000

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Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

Chalain

Phillips

Hinek

1999

J. Biomed. Mater. Res.

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Transplantation of isolated chondrocytes has long been acknowledged as a potential method for rebuilding small defects in damaged or deformed cartilages. Recent advances in tissue engineering permit us to focus on production of larger amounts of cartilaginous tissue, such as might be needed for reconstructive surgery of the entire auricle. In this report we describe modification of the basic techniques that lead to production of a large amount of elastic cartilage originated from porcine and human isolated chondrocytes. Small fragments of auricular cartilage were harvested from children undergoing ear reconstruction for microtia or extirpation of preauricular tags and from ears of juvenile pigs. Enzymatically isolated elastic chondrocytes were then agitated in suspension to form the chondronlike aggregates, which were further embedded in molded hydrogel constructs made of alginate and type I collagen augmented with kappa-elastin. The constructs were then implanted in nude mice and harvested 4 and 12 weeks after heterotransplantation. The resulting neocartilage closely resembled native auricular cartilage at the gross, microscopic, and ultrastructural levels. Immunohistochemistry and electron microscopy additionally confirmed that the newly produced cartilage contained the major components of the elastic cartilage-specific matrix, including collagen type II, proteoglycans, and well-assembled elastic fibers.

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Immune response by host after allogeneic chondrocyte transplant to the cartilage

Moskalewski

Hyc

Osiecka-Iwan

2002

Microscopy Res & Technique

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Chondrocytes constitutively express class I and, in some species, class II major histocompatibility complex (MHC). It is also possible that they possess specific differentiation antigen(s). Furthermore, lymphocytic cells, corresponding to NK cells, display spontaneous cytotoxic activity against chondrocytes. Studies on articular cartilage repair by transplants of allogeneic chondrocytes were mainly done on non-inbred animals, such as rabbits and hens. Surprisingly, only in single instances these transplants were rejected. In inbred rats, allogeneic chondrocytes transplanted into full-thickness defects in articular cartilage immediately after isolation evoked systemic immunological reaction and produced cartilage was rejected. Combined immunosuppression with cyclosporin A and cladribine did not prevent rejection of such transplants. Mechanical separation of transplants from bone marrow prevented sensitization of recipients and rejection of the produced cartilage. Successful allogeneic chondrocyte transplants in rabbits and hens could be tentatively explained by a certain degree of inbreeding among experimental animals, by the use of chondrocytes cultivated before grafting in artificial scaffolds and thus protected by matrix produced in vitro, and also by creation of a temporary mechanical barrier between transplant and bone marrow by tissues damaged during preparation of the defect.

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Immunological Response against Allogeneic Chondrocytes Transplanted into Joint Surface Defects in Rats

Cited by 11 publications

References 21 publications

Mechanical Barrier as a Protection against Rejection of Allogeneic Cartilage Formed in Joint Surface Defects in Rats

Mechanical Barrier as a Protection against Rejection of Allogeneic Cartilage Formed in Joint Surface Defects in Rats

Bioengineering of elastic cartilage with aggregated porcine and human auricular chondrocytes and hydrogels containing alginate, collagen, and ?-elastin

Immune response by host after allogeneic chondrocyte transplant to the cartilage

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