Immunological relationships betweenSalmonella flagella and their potential application for salmonellae detection by immunoassay

Ibrahim, G. F.; Fleet, Graham H.; Lyons, M. J.; Walker, R A

doi:10.1007/bf02123230

Cited by 19 publications

(14 citation statements)

References 19 publications

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“…ELISA of this antiserum after absorption with heterologous flagella suggested that, as is the case with Salmonella flagella (14), a proportion of these surface-exposed epitopes were crossreactive. The immunodominant surface-exposed Campylobacter flagellin epitopes were, however, serospecific and readily demonstrated by immunoprecipitation.…”

Section: Discussionmentioning

confidence: 90%

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Location of epitopes on Campylobacter jejuni flagella

Logan¹,

Trust²

1986

J Bacteriol

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Flagella were isolated from strains of Campylobacterjejuni belonging to different heat-labile serogroups and from a strain of Campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (Mr) were approximately 62,000. The flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the C. jejuni flagellins were structurally similar, and differed from C. fetus flageUin. Immunochemical analysis by Western blotting, enzyme-linked immunosorbent assay, immune electron microscopy, and immunoprecipitation with polyclonal and monoclonal antibodies revealed the presence of both internal and surface-exposed epitopes. The internal epitopes were antigenically cross-reactive and linear, and in the case of C. jejuni flagellin were located on cyanogen bromide peptides of apparent Mr 22,400 and 11,000. Antigenically cross-reactive epitopes were also present on an Mr 43,000 cyanogen bromide peptide of C. fetus flageilin. The Mr 22,400 peptide of C. jejuni VC74 flageilin also carried closely positioned internal linear epitopes for two monoclonal antibodies. One epitope was strain specific, while the other was shared by some but not all Campylobacter flageilins. The flagella of C. jejuni VC74 also displayed both surface-exposed antigenically cross-reactive and surface-exposed serospecific epitopes. Both linear and conformational epitopes contributed to the serospecificity of C. jejuni VC74 flagella, and a linear serospecific epitope was located on a cyanogen bromide peptide of apparent Mr 4,000.

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Section: Discussionmentioning

confidence: 90%

“…Trust, unpublished data). Conformational epitopes also play a major role in the serospecificity of Salmonella flagella (14,15).…”

Section: Discussionmentioning

confidence: 99%

Location of epitopes on Campylobacter jejuni flagella

Logan¹,

Trust²

1986

J Bacteriol

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“…A sensibilidade dos anti-soros testados neste trabalho foi concordante com a observada em vários outros trabalhos realizados com diferentes técnicas imunoenzimáticas e sorotipos de Salmonella [15,20,21,26]. Nestes estudos, a sensibilidade do teste imunoenzimático variou de 10 2 a 10 6 UFC/mL.…”

Section: Tabela 1 Desempenho Do Teste Imunoenzimático Na Detecçãounclassified

Produção De Imunorreagentes Para Uso Em Um Teste Imunoenzimático De Detecção De Salmonella Em Alimentos

Reis

Mamizuka

Franco

2001

Ciênc. Tecnol. Aliment.

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RESUMOO objetivo deste trabalho foi o desenvolvimento de imunorreagentes policlonais convenientes para uso em um teste imunoenzimáti-co para detecção rápida de Salmonella em alimentos. Foram produzidos seis anti-soros policlonais anti-Salmonella contendo as aglutininas e, h; 1,6; i; 1,2; f,g,s e m,t. Como antígenos, foram empregadas culturas formolizadas de quatro sorotipos de Salmonella (S. Typhimurium fases 1 e 2, S. Anatum fases 1 e 2, S. Agona monofásica e S. Oranienburg monofásica) cultivadas em caldo tripticase de soja. O teste imunoenzimático utilizado foi o indireto, empregando-se anti-IgG-peroxidase como conjugado e OPD-H 2 O 2 como sistema cromógeno. Os testes imunoenzimáticos foram conduzidos com os anti-soros policlonais individuais absorvidos e não-absorvidos, bem como empregandose um anti-soro polivalente, correspondente a um "pool" de antisoros absorvidos e não-absorvidos com culturas puras de Salmonella e outras enterobactérias. A absorção dos soros eliminou as reações cruzadas com todas as enterobactérias testadas nesse estudo, apresentadas tanto por alguns anti-soros individuais quanto pelo polivalente. Entre os seis anti-soros estudados, os que apresentaram melhor desempenho foram o f,g,s não-absorvido e o polivalente absorvido.Palavras-chave: imunorreagentes; Salmonella; ensaio imunoenzimático.

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“…In the case of H antigen, preparation of specific antiserum was facilitated by the use of purified flagellin for immunization, although the presence of conserved regions in the molecule among different serovars originated possible cross-reactions depending on the procedure used for antiserum generation [25,26]. Upon the widespread use of techniques for Monoclonal Antibody (MAb) generation, several MAbs for strain serotyping have been described [27,28].…”

Section: Introductionmentioning

confidence: 99%

Typhoid Fever as a Challenge for Developing Countries and Elusive Diagnostic Approaches Available for the Enteric Fever

Bharmoria¹

2017

IJVR

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Salmonella typhi is the causal organism for the typhoid fever. Typhoid disease is a problem in those developing countries where lack of sanitation, poor water supplies and exposure to unhygienic and polluted environment is a part of routine life of majority of population. Salmonella typhi expresses various virulence antigens like H-antigens, O-antigens and Vi antigens which plays vital role in the infection and pathogenesis of the bacteria. Salmonella typhi causes fever, nausea, headache, body fatigue and bowel related complications in the patients. This disease had been diagnosed by culture method, Widal test for past several decades. These diagnostic techniques are being replaced by the modern assays like ELISA, Dot blot ELISA, DipStick Assays, RT-PCR and other molecular assays. The evolution in diagnostic assays had occurred as the antibiotic resistance in the Salmonella typhi had shown an increase. The Salmonella typhi had been evolved into a multidrug resistant bacteria which need to be timely diagnosed to start the appropriate and correct treatment of typhoid to prevent further complications. This article imparts a brief glimpse on Salmonella typhi, typhoid fever and diagnostic assays available at clinical laboratories. There is a need to focus on the correct and rapid diagnostic methods which should be modified and made accessible to the clinical diagnostic laboratories.

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Immunological relationships betweenSalmonella flagella and their potential application for salmonellae detection by immunoassay

Cited by 19 publications

References 19 publications

Location of epitopes on Campylobacter jejuni flagella

Location of epitopes on Campylobacter jejuni flagella

Produção De Imunorreagentes Para Uso Em Um Teste Imunoenzimático De Detecção De Salmonella Em Alimentos

Typhoid Fever as a Challenge for Developing Countries and Elusive Diagnostic Approaches Available for the Enteric Fever

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