Immunological relationship between strains of Theileria anmdata Dschunkowsky and Lihs 1904

Gill, B. S.; Bansal, Gulshan; Bhattacharyulu, Y.; Kaur, Devinder; Singh, Avtar

doi:10.1016/s0034-5288(18)32692-4

Cited by 37 publications

(14 citation statements)

References 2 publications

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“…These results, which are in accord with those reported in India by Gill et al (1980) and Subramanian et al (1987), who described a significant heterologous immunity between different stocks of T. annulata, tend to confirm that in North Africa, cross-protection between non-attenuated stocks of T. annulata appears as the dominant feature.…”

Section: Discussionsupporting

confidence: 92%

A preliminary study on the attenuation of Tunisian schizont-infected cell lines of Theileria annulata

Darghouth¹,

Miled

Bouattour

et al. 1996

Parasitology Research

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Four Theileria annulata cell lines were characterised at low passage levels using two polymorphic markers and then used to infect calves. Their virulence seemed to be related to the number of genotypes present within the cell line. In all, 3 of the 4 cell lines were cultured up to passage 100 or 200 and inoculated into calves. Their characterisation using the same markers indicated that the attenuation was related to a reduction in the parasite polymorphism down to a single genotype. The immunogenicity of the three attenuated cell lines was assessed in calves using two types of challenge. Optimal protection was observed against homologous challenges. The level of immunity to heterologous challenges appeared to decrease with attenuation and seemed to depend on the cell line used.

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Section: Discussionsupporting

confidence: 92%

A preliminary study on the attenuation of Tunisian schizont-infected cell lines of Theileria annulata

Darghouth¹,

Miled

Bouattour

et al. 1996

Parasitology Research

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“…Sergent et al (1945) noted that immunity to a challenge with a homologous stock of T. annulata was stronger than that of a heterologous stock although it was not clear whether this may be attributed to antigenic differences between the stocks or simply differences in virulence. In a more recent study (Gill et al 1980), it was shown that variable, but effective, crossprotection was achieved between different T. annulata stocks of similar virulence. This would obviously have important implications as regards vaccination, since lack of crossprotection between various T. parua strains is an important limiting factor in vaccination against East Coast fever (Irvin et al 1983).…”

Section: Discussionmentioning

confidence: 99%

The development and specificity of cytotoxic cells in cattle immunized with autologous or allogeneicTheileria annulata–infectedlymphoblastoid cell lines

Innes

Millar

Brown

et al. 1989

Parasite Immunology

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Two groups of animals were immunized with either 10(6) autologous or 10(6) allogeneic Theileria annulata-infected lymphoblastoid cells cultured in vitro. The development and specificity of cytotoxic cells generated in vivo were measured throughout immunization and challenge using a panel of target cells that were either Theileria-infected or uninfected blast cells of known bovine lymphocyte antigen (BoLA) specificities. After inoculation of the cell lines the two groups showed distinct differences in both their clinical responses and the target specificity of the cytotoxic cells detected. The allogeneic T. annulata cell line recipients showed a very mild clinical response, and on day 9 after inoculation a strong cytotoxic response was detected. The response appeared to be directed against the allogeneic major histocompatibility complex (MHC) antigens of the inoculated cell line in some form of graft rejection response. By day 23 the predominant cytotoxic response was directed against the recipient animals' own cells infected with the parasite. In contrast, the autologous T. annulata cell line recipients showed very severe clinical reactions, and low levels of cytotoxicity were detected. The cytotoxicity was directed against parasite-infected targets but did not appear to be MHC restricted until day 20. Both groups were immune to a heterologous sporozoite challenge that proved lethal to two susceptible control animals, and on day 10 after challenge a peak of cytotoxicity was detected which was directed against the autologous infected target cell. This would suggest that this cytotoxic response was MHC restricted and was also cross-reactive between the heterologous parasite stocks used.

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“…Group 1: Immunized with 10 6 T. annulata macroschizont infected cells (Hisar strain, Gill et al 1980), generated in vitro from the skin donor animal as previously described (Brown 1983).…”

Section: Skin Grafting and Immunizationmentioning

confidence: 99%

“…Gharb strain sporozoites (Ouhelli 1985)) and monitored for 28 days. Animals immunized with one strain of the parasite are immune to challenge with a different strain (Gill et al 1980). Four out of six of the skin grafted animals were susceptible to challenge, while only one of six of the nongrafted animals were not protected ( Table 1).…”

Section: Susceptibility To Challenge Correlates With Cell Line Re-isomentioning

confidence: 99%

Allograft responses can interfere with the development of immunity against Theileria annulata following vaccination with parasite infected cell lines

Nichani

Brown

Campbell

et al. 1997

Parasite Immunology

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Theileria annulata macroschizont-infected cell lines are successfully used as vaccines in several countries. The inoculated animals produce a strong allogeneic response against the MHC antigens of the immunizing cell line followed by an anti-parasite response. Immunity against the parasite wanes in the absence of challenge and re-immunization is sometimes recommended. However, it is not known if allogeneic responses generated by the first immunization with a T. annulata infected cell line will interfere with the boosting of immunity against the parasite at the time of re-immunization with the same cell line. Animals were primed against MHC antigens by skin grafting, followed by immunization with a T. annulata infected cell line prepared from the skin donor. A strong anti-MHC response was produced. This interfered with parasite transfer and the development of an anti-parasite immune response; the effect was more marked when a low vaccine cell dose was used. There was a negative correlation between the ease of isolating infected cells from the animals after cell line immunization, and the subsequent response to challenge. Where no cell lines could be isolated, the animals were fully susceptible to sporozoite challenge. These observations are of immediate importance in endemic areas where cell lines of T. annulata schizonts are being used as vaccines to control the disease.

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Immunological relationship between strains of Theileria anmdata Dschunkowsky and Lihs 1904

Cited by 37 publications

References 2 publications

A preliminary study on the attenuation of Tunisian schizont-infected cell lines of Theileria annulata

A preliminary study on the attenuation of Tunisian schizont-infected cell lines of Theileria annulata

The development and specificity of cytotoxic cells in cattle immunized with autologous or allogeneicTheileria annulata–infectedlymphoblastoid cell lines

Allograft responses can interfere with the development of immunity against Theileria annulata following vaccination with parasite infected cell lines

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