Immunological Properties of Lipopolysaccharide from a Marine Bacterium, Photobacterium phosphoreum PJ‐1

Immunochemical Comparisons among Lipopolysaccharides from Symbiotic Luminous Bacteria Isolated from Several Luminous Marine Animals

“…The preparations also contained 2 to 8% nucleic acid and 0.4 to 1.6% protein except that VG-I LPS fell out of the range with 18.8%. Of these LPS, we reported the chemical and biological properties of PJ-I LPS in a previous paper (7).…”

“…First, we performed comparative double immunodiffusion tests with these LPS and the antisera against the above bacteria according to the method of Ouchterlony (9). Antisera against LPS from these symbiotic luminous bacteria were prepared by injecting heat-killed (100 C, 2 hr) cells at a density of 10 5 or 10 9 cells per ml into rabbits as described previously (7). As shown in Fig.…”

Chemical and Biological Properties of Lipopolysaccharides from Symbiotic Luminous Bacteria from Several Luminous Marine Animals

“…(17) in 0.6-x8-cm gels of 5.8% polyacrylamide containing 0.21 % N,N'-methylene bisacrylamide, 0.025% N,N,N',N'-tetramethylethylenediamine, 1% SDS, and 0.15% ammonium persulfate. The conditions for the electrophoresis of LPS have been previously reported (21). These gels were stained for carbohydrate with the periodate-Schiff reagent according to the procedure of DiRienzo and MacLeod (6).…”

“…The analytical procedures were performed as previously described (21). Total carbohydrate was determined by the phenol-H 2S04 method (8).…”

“…Recently, we reported that the lipopolysaccharide (LPS) from the symbiotic luminous marine bacterium Photobacterium phosphoreum PJ-l is characterized by the low content of2-keto-3-deoxyoctonate (KDO; 0.2%, wjw) and has potent and diverse biological activities similar to those of LPS of the terrestrial gram-negative bacteria Salmonella typhimurium and Escherichia coli 0111 : B4 (20,21). Moreover, we reported that four of the LPS preparations from the symbiotic luminous bacteria isolated from six species of luminous animals employed in the present study did not form any 137 precipitin line with heterologous anti-LPS serum (19).…”