Immunological profile of periapical endodontic infection in patients undergoing haematopoietic transplantation

Diniz, Julia Mourão Braga; Espaladori, Marcela Carvalho; Silva, Maria Elisa E Souza; Brito, Luciana Carla Neves de; Vieira, Leda Quércia; Sobrinho, Antônio Paulino Ribeiro

doi:10.1007/s00784-020-03448-5

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…Increased mRNA expression of CCL-2, an anti-inflammatory factor responsible for initial immune cell recruitment [ 48 ], angiogenesis, and homeostasis [ 85 ] however, could be detected on the soft and intermediate substrates rather than the stiff ones. Overall, it is tempting to speculate that DPSCs serve as trophic mediators in vivo.…”

Section: Discussionmentioning

confidence: 99%

Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering

et al. 2023

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Dental pulp regeneration strategies frequently result in hard tissue formation and pulp obliteration. The aim of this study was to investigate whether dental pulp stem cells (DPSCs) can be directed toward soft tissue differentiation by extracellular elasticity. STRO-1-positive human dental pulp cells were magnetically enriched and cultured on substrates with elasticities of 1.5, 15, and 28 kPa. The morphology of DPSCs was assessed visually. Proteins relevant in mechanobiology ACTB, ITGB1, FAK, p-FAK, TALIN, VINCULIN, PAXILLIN, ERK 1/2, and p-ERK 1/2 were detected by immunofluorescence imaging. Transcription of the pulp marker genes BMP2, BMP4, MMP2, MMP3, MMP13, FN1, and IGF2 as well as the cytokines ANGPT1, VEGF, CCL2, TGFB1, IL2, ANG, and CSF1 was determined using qPCR. A low stiffness, i.e., 1.5 kPa, resulted in a soft tissue-like phenotype and gene expression, whereas DPSCs on 28 kPa substrates exhibited a differentiation signature resembling hard tissues with a low cytokine expression. Conversely, the highest cytokine expression was observed in cells cultured on intermediate elasticity, i.e., 15 kPa, substrates possibly allowing the cells to act as “trophic mediators”. Our observations highlight the impact of biophysical cues for DPSC fate and enable the design of scaffold materials for clinical pulp regeneration that prevent hard tissue formation.

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Section: Discussionmentioning

confidence: 99%

Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering

et al. 2023

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“…Bone resorption is a frequent property of asymptomatic AP due to the secretion of mediators that induce osteoclastic activity [5] and is conducted by proinflammatory cytokines, such as IL-1β, TNF-α and interferongamma (IFN-γ) [6]. These mediators are responsible for bone resorption and remodelling of the progress of periapical tissues [7,8]. INF-γ is produced by Type 1 T-helper (Th1) cells.…”

Section: Introductionmentioning

confidence: 99%

“…The activated monocytes, T lymphocytes and macrophages release TNF-α as a proinflammatory cytokine and have a critical function in the immune response, differentiation, growth regulation, physiological behaviour and survival of a group of cells. TNF-α has the potential to induce the secretion of other cytokines, inflammatory mediators and enzymes [8]. High levels of TNF-α have been detected in periapical exudate [24].…”

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confidence: 99%

Effects of Nd: YAG laser on tumour necrosis factor‐alpha, interleukin‐1beta and interferon‐gamma levels in teeth with apical periodontitis: A clinical study

Baris,

Turkyilmaz,

Derici

2023

Aust Endodontic J

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This study aimed to evaluate the levels of tumour necrosis factor‐alpha (TNF‐α), interleukin‐1beta (IL‐1β) and interferon‐gamma (IFN‐γ) with asymptomatic apical periodontitis (AP). A total of 60 participants were randomly divided into two groups: the conventional irrigation (control) and the Nd: YAG laser irradiation. The interstitial fluids were obtained after root canal cleaning (day 0) and 1 week later (day 7). The TNF‐α, IL‐1β and IFN‐γ levels were assayed using the enzyme‐linked immunosorbent assay. The Mann–Whitney U, continuity correction chi‐square, Pearson chi‐square and Fisher exact tests were used. An increased level of cytokines on day 7 in the control group was observed, without statistically significant differences (p > 0.05). All cytokine levels decreased over time in the laser group. Only the IL‐1β level showed a significant difference (p < 0.05). Nd: YAG irradiation has a positive effect on decreasing the proinflammatory cytokine level and may help to control infection in teeth with AP.

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Selenium intracanal dressing: effects on the periapical immune response

et al. 2020

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Immunological profile of periapical endodontic infection in patients undergoing haematopoietic transplantation

Cited by 6 publications

References 39 publications

Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering

Biomechanical Modulation of Dental Pulp Stem Cell (DPSC) Properties for Soft Tissue Engineering

Effects of Nd: YAG laser on tumour necrosis factor‐alpha, interleukin‐1beta and interferon‐gamma levels in teeth with apical periodontitis: A clinical study

Selenium intracanal dressing: effects on the periapical immune response

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