Immunological examination of peripheral blood in patients with colorectal cancer compared to healthy controls

Špaček, Jan; Vočka, Michal; Netíková, Irena; Skálová, Helena; Dundr, Pavel; Konopásek, Bohuslav; Závadová, Eva; Petruželka, Luboš

doi:10.1080/08820139.2018.1480030

Cited by 24 publications

(18 citation statements)

References 40 publications

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“…It is well known that immunity decreases with aging and the number of immune cells in cancer patients changes with the development of the disease [25,26]. In the present study, we found that the circulating NK cells were positively correlated to the T and B lymphocytes, which are important immune cells of the human immune system [5][6][7].…”

Section: Discussionsupporting

confidence: 62%

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Tang

Xie

et al. 2020

BMC Gastroenterol

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Background: The association between natural killer (NK) cells and survival in colorectal cancer (CRC) patients remains controversial. This study aimed to clarify the prognostic value of peripheral blood NK cells in CRC patients.Methods: A total of 447 CRC patients who underwent radical surgery and chemotherapy were retrospectively analyzed. Cox regression analyses were used to identify independent prognostic indicators. Correlation between NK cell percentage and other clinicopathological features (gender, age, histological grade, tumor stage, immune cells, and inflammatory indicators) was analyzed. The prognostic values of the combinations of NK cell percentage and other clinicopathological features were also determined. Results: Multivariate Cox regression analysis revealed that NK cell percentage in the peripheral blood was an independent prognostic indicator in CRC patients. A higher percentage of NK cells indicated a longer survival time than a lower percentage. NK cell percentage was positively correlated to the T and B lymphocyte counts and negatively correlated to the patients' age and albumin levels. With an area of 0.741 under a receiver operating characteristic curve, NK cells have a moderate predictive value for 3rd-year survival in CRC. This area increased to 0.851 by combining NK cell percentage with the B lymphocyte count. Elderly patients and those at an advanced clinical stage presented a lower percentage of NK cells than younger patients and those at an early clinical stage.Conclusions: This study demonstrated that NK cells in the blood were an independent predictor of survival in CRC patients, and the combined count of NK cells and B lymphocytes could increase the prognostic value.

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Section: Discussionsupporting

confidence: 62%

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Tang

Xie

et al. 2020

BMC Gastroenterol

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“…We also discovered a higher proportion of activated NK cells in ltrated in patients with MUC4 mutated, indicating that MUC4 mutation could stimulate activation of NK cells. Studies show that NK cells were major players in the early detection, control of tumor growth and metastasis [37][38][39]. Previous studies reported that M2-tumor-associated macrophages (M2-TAMs) were common in malignant tumors, which creates a favorable microenvironment for tumor progression [40,41].…”

Section: Discussionmentioning

confidence: 99%

MUC4 mutation correlates with tumor mutation burden and the immune microenvironment in colorectal cancer

T¹,

Wang²,

Halike³

et al. 2020

Preprint

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Background: Immunotherapy is a new strategy for Colorectal cancer (CRC) treatment. Tumor mutation burden (TMB) may act as an emerging biomarker for predicting responses to immunotherapy. Nevertheless, no studies investigate if these gene mutations correlate to TMB and tumor-infiltrating immune cells. Methods: Somatic mutation data for CRC samples were obtained from The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) datasets. Then, we investigated the relationship between these mutant genes, TMB and overall survival outcomes. GSEA analysis was performed to explore the underlying mechanism of mutant gene. Finally, we further verified the connection between gene mutations and immune response.Results: We identified 17 common mutant genes shared by both two cohorts. Further analysis found that MUC4 mutation was strongly related to higher TMB and predicted a poorer prognosis. Moreover, functional enrichment analysis of samples with MUC4 mutation revealed that they were involved in regulating the natural killer cell mediated cytotoxicity signaling pathway. Significant changes in the proportion of the immune cells of CD8 T cells, activated NK cells, M1 macrophages and resting memory CD4 T cells were observed using the CIBERSORT algorithm. Conclusions: Our research revealed that MUC4 mutation significantly correlated with increased TMB, a worse prognosis and modulating the immune microenvironment, which may be considered a biomarker to predict the outcome of the immune response in colorectal cancer.

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“…We also discovered a higher level of activated NK cells in ltrated in samples with MUC4 mutated, indicating that MUC4 mutation could stimulate activation of NK cells,however, more data are needed for various kinds of tumors. Studies show that NK cells were major players in the early detection, control of tumor metastasis in colon cancer [36][37][38]. Previous studies reported that M2-tumorassociated macrophages (M2-TAMs) were common in malignant tumors, which creates a favorable microenvironment for tumor progression [39,40].…”

Section: Some Researches Have Revealed the Relationship Between Tumormentioning

confidence: 99%

Prognostic Biomarker MUC4 Mutations in Colon Adenocarcinoma Correlating with Tumor Microenvironment

Hui

Gao

2021

Preprint

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Background: Immunotherapy is a new strategy for Colorectal cancer (CRC) treatment. Tumor mutation burden (TMB) may act as an emerging biomarker for predicting responses to immunotherapy. Nevertheless, no studies investigate if these gene mutations correlate to TMB and tumor-infiltrating immune cells. Methods: Somatic mutation data for CRC samples were obtained from The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) datasets. Then, we investigated the relationship between these mutant genes, TMB and overall survival outcomes. GSEA analysis was performed to explore the underlying mechanism of mutant gene. Finally, we further verified the connection between gene mutations and immune response.Results: We identified 17 common mutant genes shared by both two cohorts. Further analysis found that MUC4 mutation was strongly related to higher TMB and predicted a poorer prognosis. Moreover, functional enrichment analysis of samples with MUC4 mutation revealed that they were involved in regulating the natural killer cell mediated cytotoxicity signaling pathway. Significant changes in the proportion of the immune cells of CD8+ T cells, activated NK cells, M1 macrophages and resting memory CD4+ T cells were observed using the CIBERSORT algorithm. Conclusions: Our research revealed that MUC4 mutation significantly correlated with increased TMB, a worse prognosis and modulating the immune microenvironment, which may be considered a biomarker to predict the outcome of the immune response in colorectal cancer.

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Immunological examination of peripheral blood in patients with colorectal cancer compared to healthy controls

Abstract: Our results show peripheral blood levels of TGF beta and VEGF were significantly increased in colorectal patients and changes in cellular anticancer immunity in comparison to control group. These results will be compared with results from Immunoscore.

Cited by 24 publications

References 40 publications

Prognostic value of peripheral blood natural killer cells in colorectal cancer

Prognostic value of peripheral blood natural killer cells in colorectal cancer

MUC4 mutation correlates with tumor mutation burden and the immune microenvironment in colorectal cancer

Prognostic Biomarker MUC4 Mutations in Colon Adenocarcinoma Correlating with Tumor Microenvironment

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