Immunological evidence for a ribulose bisphosphate carboxylase-like protein in the symbiotic dinoflagellate Symbiodinium sp.

Abstract

1. Summary Whereas previously there has been no convincing evidence for ribulose bisphosphate carboxylase in dinoflagellates, a strong and highly specific reaction was observed when antibodies to the denatured large subunit of the (silver beet) protein were used to probe Western blots of whole soluble fractions of various Symbiodinium isolates. No reaction was observed using extracts from Symbiodinium isolated from a host which had been maintained under low light intensity. The results imply extensive sequence homology between the large subunit of ribulose bisphosphate carboxylase and a dinoflagellate protein of M, approximately 35 000.

PRELIMINARY INVESTIGATIONS INTO THE STRUCTURE AND ACTIVITY OF RIBULOSE BISPHOSPHATE CARBOXYLASE FROM TWO PHOTOSYNTHETIC DINOFLAGELLATES1

“…Polyclonal antiserum to silver beet Rubisco was prepared previously (Gerberding et al 1990). Antibody to both the LSu and SSu of Rubisco from 1. galbana was kindly provided by Dr. Paul Falkowski, Brookhaven National Laboratory, Upton, New York.…”