Immunological Estimation of Inflammatory Interleukins (IL-4, IL-6 & IL-10) Among Patients with Bladder Cancer

Abstract

The aim of this study was to determine Immunomolecular expression of inflammatory interleukins (IL-4, IL-6 & IL-10) by using ELISA, conventional PCR and Sequencing technologies, to give acknowledge about the roles of these interleukins in patients with bladder cancer in Basrah province. A case-control study included 85 confirmed bladder cancer patients and 80 individual as a control group. Data about age, gender, smoking, alcohol drinking, family history, occupation, residency and clinical findings for all patients with urothelial carcinoma were collected. This study show the effect of demographical factors ( age, gender smoking, family history, alcohol drinking, occupation and residency) in patients with bladder cancer. The current study measure interleukins (IL-4, IL-6 & IL-10) concentration (pg\ml) by using ELISA technology among various age groups, gender, tumor sages (T1-T4) and case status (newly diagnosed, advance & recurrence). The results show significant increase of interleukins 4, 6 and 10 in comparison with control group (P-value= 0.008, 0.009, 0.001) respectively.