Immunological detection of Phaeoacremonium aleophilum, a fungal pathogen found in esca disease

Fleurat‐Lessard, Pierrette; Luini, Estelle; Berjeaud, Jean‐Marc; Roblin, Gabriel

doi:10.1007/s10658-013-0372-7

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…al GFP was localized in the lumen of xylem vessel and xylem fibers six and twelve weeks post inoculation [ 38 ]. This study is consistent with the immunolocalization performed four months post inoculation [ 39 ] and also confirms microscopic observation using non-specific technics to localize fungal agents [ 40 ]. Landi et al .…”

Section: Introductionsupporting

confidence: 91%

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Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli

et al. 2016

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The filamentous fungi Phaeoacremonium aleophilum (P.al, Teleomorph: Togninia minima) and Phaeomoniella chlamydospora (P.ch) are believed to be causal agents of wood symptoms associated with the Esca associated young vine decline. The occurrence of these diseases is dramatically increasing in vineyards all over the world whereas efficient therapeutic strategies are lacking. Both fungi occupy the same ecological niche within the grapevine trunk. We found them predominantly within the xylem vessels and surrounding cell walls which raises the question whether the transcriptional response towards plant cell secreted metabolites is comparable. In order to address this question we co-inoculated grapevine callus culture cells with the respective fungi and analyzed their transcriptomes by RNA sequencing. This experimental setup appears suitable since we aimed to investigate the effects caused by the plant thereby excluding all effects caused by other microorganisms omnipresent in planta and nutrient depletion. Bioinformatics analysis of the sequencing data revealed that 837 homologous genes were found to have comparable expression pattern whereas none of which was found to be differentially expressed in both strains upon exposure to the plant cells. Despite the fact that both fungi induced the transcription of oxido- reductases, likely to cope with reactive oxygen species produced by plant cells, the transcriptomics response of both fungi compared to each other is rather different in other domains. Within the transcriptome of P.ch beside increased transcript levels for oxido- reductases, plant cell wall degrading enzymes and detoxifying enzymes were found. On the other hand in P.al the transcription of some oxido- reductases was increased whereas others appeared to be repressed. In this fungus the confrontation to plant cells results in higher transcript levels of heat shock and chaperon-like proteins as well as genes encoding proteins involved in primary metabolism.

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Section: Introductionsupporting

confidence: 91%

“…al . [ 41 ][ 39 ]. Since growth in wood is extremely slow and retrieval of fungal cells difficult and very inefficient we designed an experimental setup that allows good recovery of cellular material under tightly controlled conditions.…”

Section: Discussionmentioning

confidence: 99%

Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli

et al. 2016

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“…P. chlamydospora causes more wood degradation than P. aleophilum ; however, the two strains are equally aggressive on grapevine cuttings in laboratory conditions ( Laveau et al, 2009 ). These pathogens consist of tracheomycetes, which invade xylem vessels: P. aleophilum , for example, was immuno-localized in xylem vessels, fibers, and pith 4 months after internodal inoculation ( Fleurat-Lessard et al, 2014 ), whereas P. chlamydospora was identified mainly in xylem vessels and surrounding fibers ( Valtaud et al, 2009 ; Fleurat-Lessard et al, 2010 ; Mutawila et al, 2011 ). Both fungi are likely to share the same ecological niche and their synergetic interaction has already been investigated, showing that P. chlamydospora secretes toxins affecting the plant and favoring the activity of P. aleophilum wood-degrading enzymes ( Luini et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Variations in Early Response of Grapevine Wood Depending on Wound and Inoculation Combinations with Phaeoacremonium aleophilum and Phaeomoniella chlamydospora

et al. 2016

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Defense mechanisms in woody tissue are poorly understood, especially in vine colonized by trunk pathogens. However, several investigations suggest that molecular mechanisms in the central tissue of Vitis vinifera L. may be involved in trunk-defense reactions. In this work, the perception of Phaeoacremonium aleophilum and Phaeomoniella chlamydospora alone or together were investigated in cuttings of Cabernet Sauvignon trunks. Plant responses were analyzed at the tissue level via optical microscopy and at the cellular level via plant-gene expression. The microscopy results revealed that, 6 weeks after pathogen inoculation, newly formed vascular tissue is less developed in plants inoculated with P. chlamydospora than in plants inoculated with P. aleophilum. Co-inoculation with both pathogens resulted in an intermediate phenotype. Further analysis showed the relative expression of the following grapevine genes: PAL, PR10.3, TL, TLb, Vv17.3, STS, STS8, CWinv, PIN, CAM, LOX at 10, 24, 48, and 120 h post-inoculation (hpi). The gene set was induced by wounding before inoculation with the different pathogens, except for the genes CAM and LOX. This response generated significant noise, but the expression of the grapevine genes (PAL, PR10.3, TL, TLb, Vv17.3, STS, STS8, CWinv, and PIN) still differed due to perception of mycelium by the plant. Furthermore, at 48 hpi, the induction of PAL and STS8 differs depending on the pathogen, and a specific pattern emerges from the different inductions associated with the different treatments. Based on these results, we conclude that V. vinifera L. trunk perceives the presence of pathogens differently depending on the inoculated pathogen or even on the combination of co-inoculated pathogens, suggesting a defense orchestration in the perennial organs of woody plants.

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“…Luini et al (2010) reported a polypeptide fraction secreted by P. minimum that triggered the death of grapevine 41BT cells in culture, induced the membrane depolarization of cells, induced the activation of plant secondary metabolism, predominantly anthocyanin synthesis, and acted on key enzymatic reactions that are known to participate in the elicitation process, namely NADPH oxidase and phenylalanine ammonia lyase. Polypeptides have recently been traced by immunological methods (Fleurat-Lessard et al 2014), and this has confirmed that these compounds have a significant role in the disease expression.…”

Section: Plantsmentioning

confidence: 88%

Phaeoacremonium: From esca disease to phaeohyphomycosis

et al. 2015

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Immunological detection of Phaeoacremonium aleophilum, a fungal pathogen found in esca disease

Cited by 20 publications

References 39 publications

Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli

Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli

Variations in Early Response of Grapevine Wood Depending on Wound and Inoculation Combinations with Phaeoacremonium aleophilum and Phaeomoniella chlamydospora

Phaeoacremonium: From esca disease to phaeohyphomycosis

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