Immunological cross-reactions and interactions between the Drosophila melanogaster ref(2)P protein and sigma rhabdovirus proteins

Wyers, Françoise; Dru, Philippe; Simonet, B.; Contamine, Didier

doi:10.1128/jvi.67.6.3208-3216.1993

Cited by 23 publications

(13 citation statements)

References 30 publications

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“…Among these, ref (2)P, which is necessary for male fertility (18), is the best-characterized gene. The Ref(2)P protein shares a conformation-dependent epitope with the SiV N protein and forms a complex with the viral P protein (104). This complex formation with the P protein is probably one of the mechanisms by which ref (2)P renders resistance to SiV infection, as the P protein is essential for rhabdovirus replication.…”

Section: Interaction Between Sigma Virus and Drosophilamentioning

confidence: 99%

Cellular and Molecular Aspects of Rhabdovirus Interactions with Insect and Plant Hosts

Ammar

Tsai

Whitfield

et al. 2009

Annu. Rev. Entomol.

155

153

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The rhabdoviruses form a large family (Rhabdoviridae) whose host ranges include humans, other vertebrates, invertebrates, and plants. There are at least 90 plant-infecting rhabdoviruses, several of which are economically important pathogens of various crops. All definitive plant-infecting and many vertebrate-infecting rhabdoviruses are persistently transmitted by insect vectors, and a few putative plant rhabdoviruses are transmitted by mites. Plant rhabdoviruses replicate in their plant and arthropod hosts, and transmission by vectors is highly specific, with each virus species transmitted by one or a few related insect species, mainly aphids, leafhoppers, or planthoppers. Here, we provide an overview of plant rhabdovirus interactions with their insect hosts and of how these interactions compare with those of vertebrate-infecting viruses and with the Sigma rhabdovirus that infects Drosophila flies. We focus on cellular and molecular aspects of vector/host specificity, transmission barriers, and virus receptors in the vectors. In addition, we briefly discuss recent advances in understanding rhabdovirus-plant interactions.

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Section: Interaction Between Sigma Virus and Drosophilamentioning

confidence: 99%

Cellular and Molecular Aspects of Rhabdovirus Interactions with Insect and Plant Hosts

Ammar

Tsai

Whitfield

et al. 2009

Annu. Rev. Entomol.

155

153

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“…A polymorphism in the host gene ref (2)P-where a complex mutation has transformed a glutamine-asparagine group to a single glycine-reduces the viral replication rate (Brun and Plus 1980;Contamine et al 1989;Dru et al 1993;Wayne et al 1996;Bangham et al 2007). The mechanisms of this resistance are unclear, although the ref(2)P protein is known to physically interact with the viral P protein (Wyers et al 1993). The ref (2)P polymorphism greatly reduces the rate of vertical transmission, both when the virus is transmitted by the mother through the egg and by the father through sperm (Bangham et al 2008a).…”

Section: Virusmentioning

confidence: 99%

THE DYNAMICS OF RECIPROCAL SELECTIVE SWEEPS OF HOST RESISTANCE AND A PARASITE COUNTER-ADAPTATION INDROSOPHILA

Wilfert

Jiggins

2012

Evolution

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Host-parasite coevolution can result in consecutive selective sweeps of host resistance alleles and parasite counter-adaptations. To illustrate the dynamics of this important but little studied form of coevolution, we have modeled an ongoing arms race between Drosophila melanogaster and the vertically transmitted sigma virus, using parameters we estimated in the field. We integrate these results with previous work showing that the spread of a resistance allele of the ref(2)P gene in the host was followed by the spread of a virus genotype, which overcomes this resistance. In line with these observations, our model predicts that there can be rapid selective sweeps in both the host and parasite, which can drive large changes in the prevalence of infection. The virus will tend to be ahead in the arms race, as incomplete dominance slows down host adaptation and selection for host resistance is weaker than selection for parasites to overcome resistance-the "life-dinner" principle. This asymmetry in the adaptation rates results in a partial sweep of the host resistance allele, as it loses its advantage part way through the selective sweep. This well-understood natural system illustrates how the outcome of host-parasite coevolution is determined by different population genetic parameters in the field. K E Y W O R D S :Cost of infection, dominance, life dinner principle, partial sweep, speed, selective sweep.

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“…The Ref(2)P protein is found in complexes associated with the viral P protein, which is required for RNA polymerase activity. Furthermore, the cellular protein shares conformation-dependent epitopes that are common with some of those of the structural N protein associated with the viral genome (21).…”

mentioning

confidence: 99%

“…Immunoprecipitation and Western blot (immunoblot) analysis. The preparation and purification of anti-Ref 2P protein antibodies have already been described (21). T-REF antibodies were raised against the whole permissive Ref(2)P protein expressed in recombinant baculovirus-infected Spodoptera frugiperda cells.…”

mentioning

confidence: 99%

“…Sigma virus ribonucleoprotein (RNP) antibodies were directed against the proteins associated with the viral RNP complex. The immunoprecipitation of the proteins has been described previously (21). Under denaturing conditions, the proteins were extracted from ground embryos or adult flies in 1ϫ SB (62 mM Tris-HCl [pH 6.8], 1% sodium dodecyl sulfate [SDS], and 1.25% ␤-mercaptoethanol) and a mixture of protease inhibitors called CLAPA (1 g [each] of chymostatin, leupeptin, antipain, and pepstatin per ml and 8 g of aprotinin per ml) at 100ЊC for 15 min.…”

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confidence: 99%

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Localization of domains within the Drosophila Ref(2)P protein involved in the intracellular control of sigma rhabdovirus multiplication

Wyers

Petitjean

Dru

et al. 1995

J Virol

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The ref(2)P gene of Drosophila melanogaster interferes with sigma rhabdovirus multiplication. This gene is highly variable, and the different alleles are considered permissive or restrictive according to their effects on virus replication. In all cases, the mechanisms involve intracellular interactions between the sigma virus and Ref(2)P proteins. We showed that the N-terminal domain of the Ref(2)P protein was required for its activity in vivo. The protein was inactive in the null p od2 mutant when its first 82 amino acids were deleted. The p ⌬n gene was constructed so that the first 91 amino acids coded for by the restrictive alleles could be expressed in vivo. It was active in a transformed line. This sequence was sufficient to impart a restrictive phenotype to an adult D. melanogaster fly after it was injected with the virus. However, the truncated protein expressed by p ⌬n did not have an effect on the hereditary transmission of the sigma virus to the offspring of the infected flies, even though it contained the restriction site. The native Ref(2)P protein has been previously shown to have conformation-dependent epitopes common with some of those of the viral N protein. We demonstrated the following. (i) These epitopes were found in a domain of the Ref(2)P protein distinct from the site involved in restriction. (ii) They were modified in the N protein of the haP7 sigma virus mutant selected as being adapted to the restrictive alleles of the ref(2)P gene; only one mutation in the N gene, leading to an amino acid substitution, distinguished the haP7 mutant from the original virus. (iii) The virus strains partially or totally adapted to the effects of the full restrictive protein expressed by p p were always found to multiply to a lesser extent in the presence of the protein expressed by p ⌬n. These data suggest that two distinct domains of the Ref(2)P protein are involved in the control of sigma virus multiplication.

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Immunological cross-reactions and interactions between the Drosophila melanogaster ref(2)P protein and sigma rhabdovirus proteins

Cited by 23 publications

References 30 publications

Cellular and Molecular Aspects of Rhabdovirus Interactions with Insect and Plant Hosts

Cellular and Molecular Aspects of Rhabdovirus Interactions with Insect and Plant Hosts

THE DYNAMICS OF RECIPROCAL SELECTIVE SWEEPS OF HOST RESISTANCE AND A PARASITE COUNTER-ADAPTATION INDROSOPHILA

Localization of domains within the Drosophila Ref(2)P protein involved in the intracellular control of sigma rhabdovirus multiplication

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