1976
DOI: 10.1128/iai.14.6.1297-1301.1976
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Immunological and serological diversity of Neisseria gonorrhoeae: gonococcal serotypes and their relationship with immunotypes

Abstract: Strains of gonococcus were shown to be immunologically heterologous. Serum bactericidal activity generally correlated with induced immunity to gonococcal challenge as detected by the guinea pig subcutaneous chamber model. Sera devoid of bactericidal activity reflected the lack of cross-protection in subcutaneous chambers. Factors affecting the bactericidal assay described in this report include (i) source of complement, (ii) concentration of test antigen and complement activity, and (iii) presence of calcium a… Show more

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Cited by 15 publications
(12 citation statements)
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References 8 publications
(8 reference statements)
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“…These data suggest that antibodies directed against gonococcal lipopolysaccharide were responsible for the bactericidal activity seen in the chamber fluids of immune animals in the present study and that this antibodycomplement-mediated bactericidal activity plays a major role in the protection of these chambers against N. gonorrhoeae infection. This conclusion is in agreement with suggestions made by other investigators using this animal model (5,6,27).…”
Section: Discussionsupporting
confidence: 94%
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“…These data suggest that antibodies directed against gonococcal lipopolysaccharide were responsible for the bactericidal activity seen in the chamber fluids of immune animals in the present study and that this antibodycomplement-mediated bactericidal activity plays a major role in the protection of these chambers against N. gonorrhoeae infection. This conclusion is in agreement with suggestions made by other investigators using this animal model (5,6,27).…”
Section: Discussionsupporting
confidence: 94%
“…Previous work with the guinea pig subcutaneous (s.c.) chamber model has demonstrated the ability of gonococcal ribosomal preparations to elicit a protective immune response to a subsequent challenge with Neisseria gonorrhoeae (8,9). In agreement with others (1,5,6,15,27), the protective response seen in the s.c. chamber model correlated with the presence of hemagglutinating and bactericidal antibodies in the serum of immunized animals. However, the nature of the protection in the chamber fluid has not been fully investigated.…”
supporting
confidence: 85%
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“…The bactericidal assay employed in these studies was a modification of the method of Wong et al (25): microtiter plate wells were blanked with 25 PI of diluent (0.006% MgCl2.6H20, 0.004% CaCl2.2H20, and 1% bovine serum albumin [pH 7.2]). Heat-inactivated (56°C, 30 min) test serum (25 pl) was added to the first well and serially diluted.…”
Section: (11)mentioning
confidence: 99%
“…Immediately after dilution, 25 ,ul of a 1/10 dilution of guinea pig sera previously tested as being negative for antigonococcal activity was added to each well as a p1l of a 107-colony-forming units per ml inoculum of gonococci and incubated at ambient temperature for 90 min. After the incubation, 10 p1 of the reaction mixture was plated on GC agar plates (Difco) containing VCN inhibitor (BBL Microbiology Systems), allowed to air dry for 20 min with the cover on, and incubated overnight at 370C in a 5% C02 atmosphere.…”
Section: (11)mentioning
confidence: 99%