Immunological and microbiological findings after the application of two periodontal surgical techniques: a randomized, controlled clinical trial

Kyriazis, Thomas; Gkrizioti, Sofia; Tsalikis, Lazaros; Sakellari, Dimitra; Deligianidis, Aristeides; Konstantinidis, Antonis

doi:10.1111/jcpe.12149

Cited by 4 publications

(29 citation statements)

References 28 publications

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“…The sample size of the included studies ranged from 7 30,31 to 41 patients 32 . About half of the studies 25,32–40 had a split‐mouth design. Follow‐up time points varied and ranged from 6 weeks to 12 months after surgical procedure.…”

Section: Resultsmentioning

confidence: 99%

“…Follow‐up time points varied and ranged from 6 weeks to 12 months after surgical procedure. Microbiological follow‐up time points were reported as follows: 3 months in 75% (21 out of 28), 25,28,30,32,34,36–51 6 months in 57% (16 out of 28), 8,25,27,30,32–34,36,38,39,42,44–46,50,51 12 months in 29% (8 out of 28), 8,25,27,31,33,34,42,50 9 months in 11% (3 out of 28), 25,30,34 1.5 months 26 and 2 months 52 in 7% (2 out of 28) and 4 months 52 and 10 months 35 in 4% (1 out of 28) 35 of the studies.…”

Section: Resultsmentioning

confidence: 99%

“…Studies provided detailed description of the sampling method including removal of supragingival plaque prior subgingival plaque sample collection, type of curette used and sampling site. In the majority of the studies, subgingival plaque samples were collected with a sterile periodontal curette (57%) 8,25,26,28,30,33,34,36,37,44–48,52,54 . The second most common sampling method used was sterile paper points (35%) 27,31,32,40–43,49–51 .…”

Section: Resultsmentioning

confidence: 99%

“…Four studies (4 out of 28, 14%) 34,38,39,46 did not describe data collection in regard to plaque levels in the methodology section. From the remaining 24 studies, three studies (3 out of 24, 13%) did not present the data in the article 30,49,50 and one study (1 out of 24, 4%) only reported baseline data 36 . Among the 24 studies which collected data on plaque levels, two different plaque indices were used.…”

Section: Resultsmentioning

confidence: 99%

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Influence of periodontal surgery on the subgingival microbiome—A systematic review and meta‐analysis

Krajewski

Perussolo

Gkranias

et al. 2023

J of Periodontal Research

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Objective The objective of this systematic review and meta‐analysis was to evaluate the effect of periodontal surgery on the subgingival microbiome. Background Periodontitis is a chronic inflammation of the tooth supporting tissues caused by the dysbiosis of the subgingival biofilm. It is managed through different non‐surgical and surgical treatment modalities. Recent EFP S3 guidelines recommended performing periodontal surgery as part of Step 3 periodontitis treatment after Step 1 and Step 2 periodontal therapy, with the aim to achieve pocket closure of persisting sites. Changes in the sub‐gingival microbiome may explain the treatment outcomes observed at different time points. Various microbiological detection techniques for disease‐associated pathogens have been evolved over time and have been described in the literature. However, the impact of different types of periodontal surgery on the subgingival microbiome remains unclear. Methods A systematic literature search was conducted in Medline, Embase, LILACS and Cochrane Library supplemented by manual search (23DEC2019, updated 21APR2022). Results From an initial search of 3046 studies, 28 were included according to our specific inclusion criteria. Seven microbiological detection techniques were used to analyse disease‐associated species in subgingival plaque samples: optical microscope, culture, polymerase chain reaction (PCR), checkerboard, enzymatic reactions, immunofluorescence and 16S gene sequencing. The included studies exhibited differences in various aspects of their methodologies such as subgingival plaque sample collection or treatment modalities. Clinical data showed a significant decrease in probing pocket depths (PPD) and clinical attachment loss (CAL) after periodontal surgery. Microbiological findings were overall heterogeneous. Meta‐analysis was performed on a sub‐cohort of studies all using checkerboard as a microbiological detection technique. Random effect models for Treponema denticola (T. denticola), Porphyromonas gingivalis (P. gingivalis) and Tannerella forsythia (T. forsythia) did not show a significant effect on mean counts 3 months after periodontal surgery. Notably, Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) showed a significant increase 3 months after periodontal surgery. 16S gene sequencing was used in one included study and reported a decrease in disease‐associated species with an increase in health‐associated species after periodontal surgery at 3 and 6 months. Conclusion This systematic review has shown that the effect of periodontal surgery on the changes in subgingival microbiome is heterogeneous and may not always be associated with a decrease in disease‐associated species. The variability could be attributed to the microbiological techniques employed for the analysis. Therefore, there is a need for well‐designed and adequately powered studies to understand how periodontal surgery influences the subgingival microbiome and how the individual's microbiome affects treatment outcomes after periodont...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Influence of periodontal surgery on the subgingival microbiome—A systematic review and meta‐analysis

Krajewski

Perussolo

Gkranias

et al. 2023

J of Periodontal Research

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“…Kyriazis et al ., found that there was a constant decrease in red complex periodontal pathogens after periodontal osseous surgery: Pg was reduced from 3.1 x 10 5 to 1.7 x 10 5 , Tf was reduced from 6.7 x 10 5 to 4.2 x 10 5 , while Td remained almost stable (3.43 x 10 5 to 3.95 x 10 5 ) [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiological Response to Periodontal Therapy: A Retrospective Study

Checchi¹,

Pascolo²

2018

TODENTJ

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Background: Periodontitis is a multifactorial infection caused by a complex of pathogenic bacterial species that induce the destruction of periodontal structures.Objective:The aim of this study is to evaluate the presence and bacterial load of six periodontal pathogens bacteria, measured at initial visit and after osseous surgery in patients affected by chronic periodontitis and treated between 2005 and 2007.Methods:This cohort study was carried out on a sample of 38 consecutive patients affected by severe chronic periodontitis, diagnosed at baseline on the basis of probing depths equal to 6.68 ± 1.47 mm. On each subject, a microbiological test was performed before periodontal initial therapy and after osseous surgery (one year later). Five compromised teeth were chosen for each patient (the same teeth, before and after surgery), for a total of 190 teeth. Real-time PCR based analysis computed total bacterial load of the samples and quantified six periodontal pathogens: Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum and Prevotella intermedia. Data collection was made consulting medical charts.Results:Pocket probing depth reduction after surgery was 4.50 ± 1.54 mm (p=0.0001). The mean number of sites with bleeding at baseline was 2.08 ± 1.17 and 0.58 ± 1.00 after surgery (p=0.001). The mean number of sites with suppuration at baseline was 0.26 ± 0.86 and 0 after surgery (p=0.02). Cell count of each pathogen and total cell count were significantly higher at baseline than after surgery. Almost all bacteria presented a mean percentage reduction equal to that of the total count, except for Aa and Pi, which seemed to show a greater resistance. The difference of bacterial load, both before and after surgery, between smokers and non-smokers was not statistically significant (p<0.05). A statistically significant correlation was detected between pocket probing depth variation and bleeding on probing variation before and after the surgery, controlling for age (r=0.6, p=0.001). No significant correlations were observed between pocket probing depth and bacterial loads, except for Pg (r=0.5, p=0.001), Tf (r=0.6, p=0.001) and Td (r=0.4, p=0.02).Conclusions:Reduction of presence and bacterial load of the examined periodontal pathogens bacteria after osseous surgery, along with periodontal pocket reduction, appeared to be essential to achieve and maintain periodontal stability over years.

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Cytokine levels in crevicular fluid associated with compliance during periodontal maintenance therapy

et al. 2018

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Immunological and microbiological findings after the application of two periodontal surgical techniques: a randomized, controlled clinical trial

Abstract: The choice of the periodontal surgical procedure does not seem to affect the immunological and the microbiological profile of patients with chronic periodontitis.

Cited by 4 publications

References 28 publications

Influence of periodontal surgery on the subgingival microbiome—A systematic review and meta‐analysis

Influence of periodontal surgery on the subgingival microbiome—A systematic review and meta‐analysis

Microbiological Response to Periodontal Therapy: A Retrospective Study

Cytokine levels in crevicular fluid associated with compliance during periodontal maintenance therapy

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