Immunological and Hematological Response to Local Transplantation of Stem Cells in Injured Radial Nerve of Dogs

Abstract

The current study was carried out to investigate the immunological and hematological changes due to local transplantation of human umbilical cord-mesenchymal-stem cells (HUC-MSCs) and scaffold-stem cells (SSCs) into the injured radial nerve. Therefore, three equal groups of dogs were subjected to this study; experimental (EG), positive control (PCG) and negative control (NCG). At 1st week, dogs of EG were showed an obvious mobility dysfunction. At 2nd and 4th weeks, there were apparent improvements reported on general and physical activities as well as functional ability of forelimb with the presence of slight lameness that was cured completely at 5th week. Regarding to immunobiomarkers, insignificant differences were showed at 1st week. However, significantly increase in IgG and TNF-α, and decrease in IL-10 was reported at 2nd, 4th, and 6th weeks. Regarding to hematologic parameters, significantly increases were recorded in total WBCs from 2nd week onwards, lymphocytes and neutrophils at 2nd week, monocytes at the 2nd and 4th weeks, and total RBCs at the 8th and 16th weeks. Significant differences were not reported in values of PCV and Hb throughout this study. In conclusion, HUC-MSCs and SSCs confirmed high activities in supporting of immunological and hematological responses, and in restoration of nerve function

Efficacy of Chitosan Nanoparticle Transplantation on Regeneration of Acute Spinal Cord Injury in Dogs Model

“…The histology of the hemisection spinal cord in the control group exhibited severe vacuolation of the nerve fibres in the white matter, which was linked to pro-inflammatory cytokines which result in secondary cascades of events that occur after several hours to days of spinal cord injury, including mitochondrial dysfunction, failure of aerobic energy metabolism, and eventually the production of free oxygen radicals, which lead to lipid peroxidation and increased vascular permeability, local ischemia, intraneuronal edoema, and degenerate axons. Essa et al (2020) and Fan et al (2022) have found similar results.…”

Study the Effect of Zinc Oxide Nanoparticles on Regeneration of Damaged Sciatic Nerve in Rabbits Model

“…Employing scalpel blade 15, the subcutaneous tissue and fascia lata were then excised along the same line. The biceps femur muscle has been divided cranially, and the semitendinosus muscle was separated posteriorly by blunt dissection using Mayo scissors in order to expose the sciatic nerve and cut it free from the surrounding tissues [10,19]. The nerve had been gently compressed through putting a wooden tongue depressor under it while employing artery forceps (Figure 1).…”