Immunological and cardio-vascular pathologies associated with SARS-CoV-2 infection in golden syrian hamster

Rizvi, Zaigham Abbas; Dalal, Rajdeep; Sadhu, Srikanth; Kumar, Yashwant; Shrivastava, Tripti; Gupta, Sonu Kumar; Aggarwal, Suruchi; Tripathy, Manas Ranjan; Yadav, Amit Kumar; Medigeshi, Guruprasad R.; Pandey, Amit; Samal, Sweety; Asthana, Shailendra; Awasthi, Amit

doi:10.1101/2021.01.11.426080

Cited by 10 publications

(19 citation statements)

References 78 publications

(158 reference statements)

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“…1D & 1E). As reported earlier, splenomegaly is one of the critical parameters indicative of active infection 20 . Thus we tested the splenomegaly between different groups and found that AO, but not TT, showed inhibition in splenomegaly as compared to the SARS-CoV2 infected hamsters (Fig.…”

Section: Prophylactic Use Of Intranasal Instillation Of Ayush Oil Formulations Prevents Sars-cov2 Infection and Associated Gross Clinicalmentioning

confidence: 77%

“…Hamsters receiving Anu Oil or Til tailya intranasally before SARS-CoV2 infection showed reduced weight loss. It has been shown earlier that even low dose of SARS-CoV2 intra-nasal infection in hamster could result in pneumonitis and lung pathologies 20 . Therefore, it might be possible that even though both Til tailya and Anu Oil can limit SARS-CoV2 entry and replication by some degree but only Anu oil group showed a greater reduction in the lung viral load (around 3 folds).…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, we describe the efficacy of prophylactic use of two intra-nasal Ayurvedic oil formulations using hamster model for SARS-CoV2 challenge. Hamsters are one of the best small animal models for SARS-CoV2 infection which mimic the viral entry, replication of the upper and lower respiratory tract of humans 20,21 . Hamsters receiving Anu Oil or Til tailya intranasally before SARS-CoV2 infection showed reduced weight loss.…”

Section: Discussionmentioning

confidence: 99%

“…SARS-CoV2 infection in hamsters peaks between 4-5 days and is characterized by a reduction in body weight and appearance of pneumonitis in the lungs and splenomegaly [19][20][21][22] . These defined gross clinical parameters were recorded between all the groups i.e.…”

Section: Prophylactic Use Of Intranasal Instillation Of Ayush Oil Formulations Prevents Sars-cov2 Infection and Associated Gross Clinicalmentioning

confidence: 99%

“…Since gross clinical parameter viral load data suggested protection from SARS-CoV2 infection in AO and TT groups, we set out to study the mitigation of pulmonary pathologies such as lung injury, alveolar epithelial injury, bronchitis, pneumonitis and inflammation using histological analysis 20,[25][26][27] . Haematoxylin and eosin (H & E) stained lung data showed a reduction in alveolar epithelial injury, inflammation and pneumonitis in SARS-CoV2 infected-AO treated hamsters as compared to SARS-CoV2 infected hamsters.…”

Section: Prophylactic Use Of Anu Oil Reduces Sars-cov2-induced Lung Pathology In Hamstersmentioning

confidence: 99%

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Effect of prophylactic use of intra-nasal oil formulations in the hamster model of Covid-19

Rizvi¹,

Tripathy²,

Sharma³

et al. 2021

Preprint

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infection initiates with viral entry in upper respiratory tract leading to coronavirus disease 2019 (Covid-19). Severe Covid-19 is characterized by pulmonary pathologies associated with respiratory failure. Thus, therapeutics aimed at inhibiting entry of the virus or its internalization in the upper respiratory tract, are of interest. Herein, we report the prophylactic application of two intra-nasal formulations provided by the National Medicinal Plant Board (NMPB), Anu oil and Til tailya in SARS-CoV2 infection hamster model. Prophylactic nasal instillation of these oil formulations exhibited reduced viral load in lungs, and resulted in reduced body weight loss and pneumonitis. In line with reduced viral load, histopathlogical analysis revealed a reduction in lung pathology in Anu oil group as compared to the control infected group. However, Til tailya group did not show a significant reduction in lung pathology. Furthermore, molecular analysis using mRNA expression profiling indicated reduced expression of pro-inflammatory cytokines genes, including Th1 and Th17 cytokines for both the intra-nasal formulations as a result of decreased viral load. Together, the prophylactic intra-nasal application of Annu oil seems to be useful in limiting both the viral load and disease severity disease in SARS-CoV2 infection in hamster model.

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Section: Prophylactic Use Of Intranasal Instillation Of Ayush Oil Formulations Prevents Sars-cov2 Infection and Associated Gross Clinicalmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Prophylactic Use Of Intranasal Instillation Of Ayush Oil Formulations Prevents Sars-cov2 Infection and Associated Gross Clinicalmentioning

confidence: 99%

Section: Prophylactic Use Of Anu Oil Reduces Sars-cov2-induced Lung Pathology In Hamstersmentioning

confidence: 99%

See 3 more Smart Citations

Effect of prophylactic use of intra-nasal oil formulations in the hamster model of Covid-19

Rizvi¹,

Tripathy²,

Sharma³

et al. 2021

Preprint

Self Cite

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show abstract

Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection

et al. 2021

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SARS-CoV-2 has caused a global pandemic of COVID-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also spread to central nervous system leading to neurological sequelae. We have developed and characterized two new organotypic cultures from hamster brainstem and lung tissues that offer a unique opportunity to study the early steps of viral infection and screening antivirals. These models are not dedicated to investigate how the virus reaches the brain. However, they allow validating the early tropism of the virus in the lungs and demonstrating that SARS-CoV-2 could infect the brainstem and the cerebellum, mainly by targeting granular neurons. Viral infection induces specific interferon and innate immune responses with patterns specific to each organ, along with cell death by apoptosis, necroptosis, and pyroptosis. Overall, our data illustrate the potential of rapid modeling of complex tissue-level interactions during infection by a newly emerged virus.

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An Untargeted LC–MS based approach for identification of altered metabolites in blood plasma of rheumatic heart disease patients

Das

Kumar

Sharma

et al. 2022

Sci Rep

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Rheumatic heart disease (RHD) is often considered as a disease of developing countries and India is the home of about 40% of RHD patients. Environment seems to play a major role in its causation. Since gene environment interactions can lead to alterations of various metabolic pathways, identification of altered metabolites can help in understanding the various pathways leading to RHD. Blood plasma samples from 51 RHD and 49 healthy controls were collected for the study. Untargeted metabolomics approach was used to identify the metabolites that are altered in RHD patients. Data showed 25 altered metabolites among RHD patients. These altered metabolites were those involved in Purine, Glutamine, Glutamate, Pyrimidine, Arginine, Proline and Linoleic metabolism. Thus, the present study illuminates metabolic alterations among RHD patients which can help in determining the potential therapeutic targets.

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Immunological and cardio-vascular pathologies associated with SARS-CoV-2 infection in golden syrian hamster

Cited by 10 publications

References 78 publications

Effect of prophylactic use of intra-nasal oil formulations in the hamster model of Covid-19

Effect of prophylactic use of intra-nasal oil formulations in the hamster model of Covid-19

Hamster organotypic modeling of SARS-CoV-2 lung and brainstem infection

An Untargeted LC–MS based approach for identification of altered metabolites in blood plasma of rheumatic heart disease patients

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