Immunological Analysis of Mycotoxins

Wilkinson, A. W.; Ward, C. M.; Morgan, Michael R. A.

doi:10.1007/978-3-662-02783-7_7

Cited by 12 publications

(6 citation statements)

References 82 publications

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“…The widespread occurrences of mycotoxins in foods and feeds pose a serious health threat to humans and animals; the most toxic of these mycotoxins are T-2 toxin (T-2), ochratoxin A (OA), aflatoxin Bi (AFBi), and possibly citrinin (CTN) (CAST, 1989). Immuno-based assays have been developed for the detection and quantification of mycotoxins (Pestka, 1988; Chu, 1990Chu, , 1992Candlish, 1991;Wilkinson et al, 1992). Mycotoxins by themselves are incapable of eliciting immune responses due to their small size but can be rendered immunogenic by covalent coupling to large molecular weight carrier proteins (Harlow and Lane, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…Some mycotoxins such as OA contain an active carboxyl group, and others such as T-2 and AFBi require the introduction of a group having a carboxyl functionality. These carboxyl groups can then be activated and covalently coupled to amine groups in proteins to form stable amide linkages (Wilkinson et al, 1992;Candlish, 1991). Several amide-bond formation reactions have been employed including those that make use of the TV-hydroxysuccinimide activated esters (NHS), water-soluble carbodiimides (WSC), and mixed anhydrides (MA).…”

Section: Introductionmentioning

confidence: 99%

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Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for Immunoassays

Xiao¹,

Clarke²,

Marquardt³

et al. 1995

J. Agric. Food Chem.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for Immunoassays

Xiao¹,

Clarke²,

Marquardt³

et al. 1995

J. Agric. Food Chem.

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“…The mycotoxins deoxynivalenol (DON), zearalenone (ZEN) and T-2 toxin (T-2) were analysed by ELISA (enzyme-linked immunosorbent assay) method (Wilkinson et al, 1992). The RIDASCREEN® quantitative test kits (R-Biopharm AG, Darmstadt, Germany), were used for the analysis.…”

Section: Methodsmentioning

confidence: 99%

Fungi and Mycotoxins in Fresh Bee Pollen

Sinkevičienė

Marcinkevičienė²,

Baliukonienė³

et al. 2020

Rural Development 2019

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The aim of the present study was to determine the concentration of microscopic fungi and selected mycotoxins in fresh bee pollen, stored for different periods. In the study, 12 pollen samples collected from the same apiary families were investigated. Bees have collected the pollen from different plants. The moisture content of fresh pollen varied between 14.2 and 22.7%. During studies, the most prevalent fungal genera of Fusarium, Penicillium, Alternaria, Mucor and yeast were found in fresh bee pollen. The amounts of microscopic fungi increased from 2.9×10 3 to 4.4×10 3 cfu g -1 as the pollen storage time increased. The significantly higest amounts of fungal colonies was determined after 3 days storage of undried pollen. The most significant Fusarium spp. increase (14.9%) was determined after 2 days of storage. The highest levels of mycotoxins ZEN and DON were determined after 3 days of pollen storage.

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“…DON content in grain was estimated by a competitive direct enzymelinked immunosorbent assay (CD-ELISA) method (Wilkinson et al, 1992). Quantitative test kits Veratox® ("Neogen", UK) were used for the analysis.…”

Section: Methodsmentioning

confidence: 99%

Distribution of B type trichothecene producing Fusarium species in wheat grain and relation to mycotoxins DON and NIV concentrations

Supronienė

Sakalauskas

Mankevičienè

et al. 2016

Zemdirbyste-Agriculture

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Fusarium head blight (FHB) is one of the most important cereal diseases causing yield losses and reducing its quality. B type trichothecenes (TRI) deoxynivalenol (DON) and nivalenol (NIV) are the main mycotoxins associated with FHB of wheat and other small-grain cereals. Usually, a particular Fusarium strain is able to produce only one type of B trichothecene. The detection of gene for NIV, DON and its acetylated derivatives 3-acetyldeoxynivalenol (3ADON) or 15-acetyldeoxynivalenol (15ADON) production is used for detection of FHB pathogens in plant material and their chemotyping. The current study presents the distribution of DON and NIV mycotoxins and their potential producers in Lithuanian wheat grain grown in 2013 and 2014. Grain samples of spring wheat (114) and winter wheat (30) were collected from 49 farms situated in 12 administrative districts of Lithuania. Fusarium species were identified and quantified by the morphological and quantitative polymerase chain reaction (qPCR) techniques. DON concentrations were estimated by the enzyme-linked immunosorbent assay (ELISA) in all collected grain samples and NIV using ultra performance liquid chromatography coupled with mass spectrometry (UPLC/MS) in 17 arbitrarily selected samples. F. graminearum, F. culmorum and F. poae were identified as species capable of producing mycotoxins DON and NIV in wheat grain. The highest DON quantities were identified in the grain of spring wheat grown in 2013 and this was clearly linked to F. graminearum DNA quantities (r = 0.783, p < 0.01). F. poae stood out as a potential NIV producer in the grain of Lithuania-grown wheat, since a positive correlation (r = 0.62, p < 0.01) between the quantities of F. poae DNA and NIV concentrations was established. F. culmorum was detected in unexpectedly small quantities in wheat grain and was found to be the potential producer of DON, but not NIV.

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Immunological Analysis of Mycotoxins

Cited by 12 publications

References 82 publications

Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for Immunoassays

Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for Immunoassays

Fungi and Mycotoxins in Fresh Bee Pollen

Distribution of B type trichothecene producing Fusarium species in wheat grain and relation to mycotoxins DON and NIV concentrations

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