Immunologic Studies of Intrinsic Factor

Precipitating Serum Antibodies to Intrinsic Factor in Pernicious Anemia

“…patients in whom a previous intake of hog I F was known, supports the hypothesis that the oral administration of hog I F may result in immunization. The figures obtained were not as high as in the preliminary group studied (12), where 9 out of 1 1 p.a. patients on oral hog I F therapy had antibodies.…”

“…Philips-Duphar, Amsterdam, specific activity 20--130mCi/mg) was added in an amount corresponding to the Bl,-binding capacity (12). A micro-version of the agar-gel diffusion technique (26) was used for the antigen-antibody precipitin reactions as described previously (12). Antigen and serum were tested undiluted and diluted 1 to 4, and each test run at least in duplicate.…”

Separation and Immunologic Characterization of Hog Intrinsic Factor in Comparison with Human Intrinsic Factor

“…7). On the other hand, component B, but not A, crossreacted with rabbit antiserum to human IF preparation and reacted with sera from patients treated with hog IF, these sera having been shown to react with a B,,-binder in hog IF preparations in previous works (16,17) 11).…”

“…T h e irnmunojmcipitin reactions were studied by a micro version (34) of the Ouchterlcny technique (24) with use of autoradiography to visualize the reactions of B,,-binding substances (15, 16).…”

“…,4nti~era, produced by immunization of rabbits with human or hog IF preparations according to an earlier described technique (15,16). In a similar way antiserum ro hog gall-bladder bile was produced, 2 ml bile being used for each injection.…”