Immunologic Significance of In Vitro Lymphocyte Responses

Daguillard, F

doi:10.1016/s0025-7125(16)32397-5

Cited by 26 publications

(26 citation statements)

References 63 publications

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“…(c) Though all cultures were standardized to contain 1 X 100 lymphocytes/culture the number of neutrophils and monocytes in SF cell cultures were three to eight times the number in blood cell cultures and this could have inhibitory effects on the proliferative We are planning to extend our in vitro observations to the in vivo system and examine the possibility of modulating the inflammatory response of experimental immune arthritis, induced by immune complexes or aggregate preparations, by means of injections of aggregate-free preparations in the SF compartment. Our failure to demonstrate significant differences in PHA responses between normal and RA blood lymphocytes would indicate that cell-mediated immunity as expressed by the TD cell function is probably intact in the peripheral blood compartment of RA patients (1). The PHA responses of SF lymphocytes, on the other hand, were poor (Table II).…”

Section: Introductionmentioning

confidence: 80%

“…The in vitro blastogenic response of human lymphoid cells upon incubation with antigens and phytomitogens is being used extensively to assess the immunocompetence of cells of various lymphoid compartments (1). It has been shown by several investigators that thymusDr.…”

Section: Introductionmentioning

confidence: 99%

“…Received for publication 27 November 1972 and in revised form 9 March 1973. dependent (TD) 1 cells respond in vitro to phytohemagglutinin (PHA), whereas bone marrow-dependent (BD) cells carry immunoglobulin receptors on their surface (1). Human gamma globulin (HGG) in its aggregated or aggregate-free forms had been shown to be immunogenic or tolerogenic respjectively both in vivo (2), and in vitro (3).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Study of the In Vitro Proliferative Responses of Blood and Synovial Fluid Leukocytes of Rheumatoid Arthritis Patients

Reynolds¹,

Abdou²

1973

J. Clin. Invest.

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Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Study of the In Vitro Proliferative Responses of Blood and Synovial Fluid Leukocytes of Rheumatoid Arthritis Patients

Reynolds¹,

Abdou²

1973

J. Clin. Invest.

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“…Blast transformation: the transformation of lymphocytes in vitro to large (blast) cells has been suggested to be a correlate of immunocompétence [9] and cell-mediated immune responses [2]. Therefore, this characteristic was utilized to exam ine the capability of lymphocytes from parasitized mice to respond to both nonspecific (PHA) and specific (PPD) stimulation.…”

Section: Analyses Of Cell Stimulationmentioning

confidence: 99%

In vitro Responses of Spleen Cells from Trichinella spiralis-Infected Mice

Molinari¹,

Ebersole²

1977

Int Arch Allergy Immunol

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The in vitro cellular immune responses of spleen cells from mice infected with Trichinella spiralis and immunized with BCG have been investigated. ICR/CD-1 mice were originally infected with 200 T. spiralis larvae 22 days prior to infection with 4 × 10⁶ viable or heat-killed mycobacteria. Analysis of the splenic cell populations indicated that significant increases in adherent cells (macrophages) were noted only in groups previously infected with the nematode; the concentration of non-adherent cells (lymphocytes) did not vary significantly among any of the experimental groups. Assay of blast cell transformation and ³H-thymidine incorporation demonstrated the ability of T. spiralis infection to potentiate in vitro cellular immune reactions. These findings support earlier in vivo studies concerning nematode-induced immunopotentiation of delayed-type hypersensitivity reactions, and provide additional evidence that infection with this nematode enhances the immune capabilities of both stimulated lymphocytes and nonspecific phagocytic cells.

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“…The results ob tained thereby often determine whether to withhold certain therapeutic procedures such as blood transfusion or live vaccina tion, which are dangerous for T cell defi cient patients [7], In the following study the influence of the thymus on both the appearance of T an tigen and the proliferative response to Con A and PHA was studied in rats deprived of the thymus influence by either thymectomy or thymectomy followed by irradiation and bone marrow reconstitution. Cells carrying T antigen were identified in the lymphoid organs through their reaction with a specific anti-rat brain serum (ARBS).…”

Section: Introductionmentioning

confidence: 99%

Immunological Activities of Rat Lymphocytes

Roy¹,

McNicoll²,

Daguillard³

1976

Int Arch Allergy Immunol

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Lymphocytes from the peripheral blood, thoracic duct, spleen and lymph nodes of normal, thymectomized (Tx) and thymectomized lethally irradiated marrow reconstituted (TxBM or B rats) Lewis rats were studied for their ability to proliferate in vitro in the presence of Con A or PHA. At the same time the lymphoid tissues of these animals were examined for the presence of B cells or T cells by immunofluorescence staining with antiserums to rat immunoglobulins or rat brain antigens (ARBS), respectively. The specificity of ARBS fort T cells was first established in both cytotoxicity and immunofluorescence studies with thymocytes as well as purified T and B cells. In various lymphoid tissues from Tx and B rats, cells carrying brain antigens were found which were unable to respond to Con A and PHA. Thymectomy has a lesser effect on the response to Con A than to PHA; this was particularly true in the thoracic duct where, following thymectomy, lymphocytes continued to respond to Con A but lost their ability to respond to PHA. This suggests that the response to PHA and Con A may belong to different cells’ subsets but that the ability to respond to either one of these mitogens may be impaired in cells carrying known T cell markers.

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Immunologic Significance of In Vitro Lymphocyte Responses

Cited by 26 publications

References 63 publications

Comparative Study of the In Vitro Proliferative Responses of Blood and Synovial Fluid Leukocytes of Rheumatoid Arthritis Patients

Comparative Study of the In Vitro Proliferative Responses of Blood and Synovial Fluid Leukocytes of Rheumatoid Arthritis Patients

<i>In vitro</i> Responses of Spleen Cells from <i>Trichinella spirali</i><i>s</i>-Infected Mice

Immunological Activities of Rat Lymphocytes

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