Immunologic histamine release from isolated human basophils during specific immunotherapy

Chyrek-Borowska, S; Kuczewska, B; Rogalewska, A; Hofman, J

doi:10.1007/bf01988014

Cited by 5 publications

(1 citation statement)

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“…It has been shown that the expression of high‐affinity IgE receptors or several function‐molecules on basophils is up‐regulated in allergic patients or after stimulation in vitro in human and mouse studies [ 60–63]. The histamine release from basophils was sometimes decreased after immunotherapy [ 64, 65]. We also found in the C2.0 group that the plasma histamine concentration after antigen challenge was suppressed.…”

Section: Discussionmentioning

confidence: 50%

Hyposensitization to allergic reaction in rDer f 2-sensitized mice by the intranasal administration of a mutant of rDer f 2, C8/119S

Yasue

Yokota

Fukada

et al. 1998

Clinical and Experimental Immunology

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SUMMARYC8/119S is a mutant of recombinant Der f 2 (rDer f 2), and lacks a disulphide bond possessed by wildtype rDer f 2. In humans and mice, C8/119S has a very weak IgE-binding capacity compared with the wild-type, but possesses a T cell reactivity comparable to that of the wild-type. C8/119S may thus be a safe immunotherapeutic agent for house dust mite allergy. The aim of the present study was to evaluate whether the intranasal administration of C8/119S could suppress an immediate allergic reaction in mice sensitized with wild-type rDer f 2, possessing an allergic activity comparable to native counterparts purified from mite extract. Seven-week-old male A/J mice were immunized with wild-type rDer f 2 four times, and then intranasally administered 0·2-2 mg of wild-type, 0·2-20 mg of C8/119S, or PBS alone, three times a week for 4 weeks. Seven days after the last administration, the mice were examined for an immediate allergic reaction. The animals administered 2 mg of C8/119S (C2.0 group) showed significantly reduced immediate bronchoconstriction provoked by the i.v. injection of 1 and 10 mg of wild-type rDer f 2, compared with the PBS-treated mice. Similar results were obtained when we examined mice 10 weeks after the last administration. The reactions in the other groups given wild-type or C8/119S also tended to decrease in severity in comparison with the animals of the PBS group. The allergic phenotypes of the T cells, B cells, and basophils in the C2.0 group were shifted to that of naive mice without immunization. We conclude that C8/119S has hyposensitizing activities in mice sensitized with wild-type rDer f 2. C8/119S may be useful for immunotherapy of house dust mite allergy.

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