Immunologic deviations in recurrent spontaneous abortion mouse model

Ansariniya, Hossein; Zare, Fateme; Mosaffa, Nariman; Idali, Farah; Shabani, Mahdi; Hadinedoushan, Hossein

doi:10.1111/aji.13631

Cited by 7 publications

(3 citation statements)

References 75 publications

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“…Recurrent spontaneous miscarriage (RSM) refers to two or more consecutive spontaneous abortions with the same spouse before the 20th week of gestation [ 1 ]. RSM accounts for approximately 2–5% of the total pregnancies [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

Sun,

Yao,

et al. 2024

Epigenetics

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Decidual macrophages are the second-largest immune cell group at the maternal-foetal interface. They participate in apoptotic cell removal, and protect the foetus from microorganisms or pathogens. Dysfunction of decidual macrophages gives rise to pregnancy complications such as preeclampsia and recurrent spontaneous miscarriage (RSM). However, the mechanisms by which decidual macrophages are involved in the occurrence of adverse pregnancy outcomes have not been elucidated. Here we integrated DNA methylation and gene expression data from decidua macrophages to identify potential risk factors related to RSM. GPR133 was significantly hypomethylated and upregulated in decidual macrophages from RSM patients. Further demethylation analysis demonstrated that GPR133 expression in decidual macrophages was significantly increased by 5-Aza-dC treatment. In addition, the influence of GPR133 on the phagocytic ability of macrophages was explored. Phagocytosis was impaired in the decidual macrophages of RSM patients with increased GPR133 expression. Increased GPR133 expression induced by demethylation treatment in the decidual macrophages of healthy control patients led to a significant decrease in phagocytic function. Importantly, knockdown of GPR133 resulted in a significant improvement in the phagocytic function of THP-1 macrophages. In conclusion, the existing studies have shown the influence of GPR133 on the phagocytic function of decidual macrophages and pregnancy outcomes, providing new data and ideas for future research on the role of decidual macrophages in RSM.

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Section: Introductionmentioning

confidence: 99%

Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

Sun,

Yao,

et al. 2024

Epigenetics

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“…Under these circumstances, it is crucial to identify factors that indicate a high risk for the presence of CE. [18][19][20][21][22] Analysis of these factors will allow targeted selection of patients for hysteroscopy or other methods of endometrial biopsy, thereby increasing the eff ectiveness of diagnostic eff orts.…”

Section: Introductionmentioning

confidence: 99%

Correlation Ratio of Chronic Endometritis With Anamnestic Factors and Ultrasound Characteristics of the Endometrium in Women of Reproductive Age

Likhachov,

Taranovska,

Akimov

et al. 2024

Neonatol. hìr. perinat. med.

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The diagnosis of chronic endometritis is problematic because of its asymptomatic course and the need for invasive uterineprocedures to confi rm the diagnosis histologically. Therefore, it is important to identify factors that indicate a high risk of this condition. Aim: The aim of this study is to identify factors that have a statistically signifi cant correlation with the presence of CE in women of reproductive age and to validate them by histologic examination of the endometrium.Material and Methods. A retrospective analysis of 400 histories of women of reproductive age who underwent hysteroscopyand endometrial biopsy for histologic examination was performed. Histologically, 154 women (38.5 %) were found to havechronic endometritis (the group of women with chronic endometritis), while 246 women (61.5 %) were found to have otherconditions unrelated to chronic endometritis (the group of women without chronic endometritis). Analysis of anamnestic factors and ultrasound criteria associated with an increased likelihood of chronic endometritis was performed when comparing data from women in these groups. The strength of the relationship between these factors on the one hand and the development of chronic endometritis on the other hand was assessed by calculating the Kendall’s τ (tau) rank correlation coeffi cient. The strength of the relationship between the mentioned factor and CE was considered strong (+++) with a τ coeffi cient of 0.5 or higher, moderate (++) with a τ coeffi cient in the range of 0.2 to 0.499, weak (+) with a τ coeffi cient in the range of 0.1 to 0.199. A τ-coeffi cient value between 0 and 0.099 indicated no correlation between the factor and chronic endometritis.The study was conducted in accordance with the principles of patient- centered care, the requirements of the Tokyo Declaration of the World Medical Association, the international recommendations of the Helsinki Declaration of Human Rights, the Convention of the Council of Europe on Human Rights and Biomedicine, the laws of Ukraine, and the Code of Ethics of the Ukrainian Physician.The paper is an excerpt from the initiative scientifi c research project of the Department of Obstetrics and Gynecology No.2 at Poltava State Medical University, entitled «Optimization of approaches to the management of pregnancy in women at high risk of obstetric and perinatal pathology» (State registration number 0122U201228, duration: 10.2022-09.2027) in collaboration with the Department of Pathological Physiology at Poltava State Medical University.Results and Discussion. The determination of the correlation coeffi cient allowed us to identify the factors with the strongestassociation with chronic endometritis. Among them are 5 infectious factors: cervicitis in the past medical history (τ coeffi cient 0.625245229; p<0.0000000001); chronic infl ammation of the uterine appendages (τ coeffi cient 0.522536031; p<0.0000000001); sexually transmitted diseases in the past medical history (τ coeffi cient 0.547218916; p<0.0000000001); chronic urogenital infl ammatory diseases in the sexual partner (τ coeffi cient 0. 529314979; p<0.0000000001); previous multiple use of intrauterine contraception (τ-coeffi cient 0.502383401; p<0.0000000001); 4 symptomatic consequential factors: infertility in past medical history (τ-coeffi cient 0.683492482; p<0.0000000001); missed miscarriages in past medical history (τ-coeffi cient 0. 644489429; p<0.0000000001); recurrent early pregnancy loss (τ-coeffi cient 0.625942138; p<0.0000000001); abnormal uterine bleeding (τ-coeffi cient 0.650850348; p<0.0000000001); and 4 ultrasound criteria, such as the presence of local endometrial thickening in the form of a polyp (τ-coeffi cient 0.641820318; p<0. 0000000001); increased echogenicity of the endometrium (τ-coeffi cient 0.665249637; p<0.0000000001); heterogeneity of the endometrial echo structure with areas of increased and decreased echogenicity (τ-coeffi cient 0.693152163; p<0.0000000001); hyperechogenic structures in the basal layer of the endometrium (τ-coeffi cient 0.521658745; p<0.0000000001). In our opinion, the combination of an infectious or symptomatic factor with an ultrasound criterion indicates a high probability of chronic endometritis. In the absence of ultrasound signs, the combination of 1 infectious and 1 symptomatic- consequential factor indicates a high risk of chronic endometritis. To confirm the representativeness of this method, we analyzed endometrial biopsies obtained by curettage in 100 women with this combination of factors. The diagnosis was confi rmed histologically in 87 women. Conclusion. Our proposed assessment of high-risk criteria for chronic endometritis, based on the combination of infectious,symptomatic- consequential, and ultrasound criteria, allows predicting the presence of this pathology in 87 % of patients withoutтthe need for invasive interventions. This helps to improve patient selection for hysteroscopy and avoid unnecessary uterine interventions in the diagnosis of chronic endometritis in women planning pregnancy.

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“…Complex immunological processes at the maternal-fetal interface have been mainly studied in rodent models. Preclinical studies have shown that the equilibrium of T-, B-and uterine natural killer (NK) cells (9) and the production of immunosuppressive cytokines by regulatory T cells (10) is important in a typical pregnancy. These models have also shown that a shift in T-helper (Th) cell balance towards increased Th1 cells, complement activation and the depletion of regulatory T cells or NK cells may all lead to adverse pregnancy outcomes, including fetal rejection, impaired fetal growth, and abnormal neurodevelopment (11)(12)(13)(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Inflammatory markers in pregnancy – surprisingly stable. Mapping trajectories and drivers in four large cohorts

Gigase,

Suleri,

Isaevska

et al. 2024

Preprint

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Adaptations of the immune system throughout gestation have been proposed as important mechanisms regulating successful pregnancy. Dysregulation of the maternal immune system has been associated with adverse maternal and fetal outcomes. To translate findings from mechanistic preclinical studies to human pregnancies, studies of serum immune markers are the mainstay. The design and interpretation of human biomarker studies require additional insights in the trajectories and drivers of peripheral immune markers. The current study mapped maternal inflammatory markers (C-reactive protein (CRP), interleukin (IL)-1β, IL-6, IL-17A, IL-23, interferon-γ) during pregnancy and investigated the impact of demographic, environmental and genetic drivers on maternal inflammatory marker levels in four multi-ethnic and socio-economically diverse population-based cohorts with more than 12,000 pregnant participants. Additionally, pregnancy inflammatory markers were compared to pre-pregnancy levels. Cytokines showed a high correlation with each other, but not with CRP. Inflammatory marker levels showed high variability between individuals, yet high concordance within an individual over time during and pre-pregnancy. Pre-pregnancy body mass index (BMI) explained more than 9.6% of the variance in CRP, but less than 1% of the variance in cytokines. The polygenic score of CRP was the best predictor of variance in CRP (>14.1%). Gestational age and previously identified inflammation drivers, including tobacco use and parity, explained less than 1% of variance in both cytokines and CRP. Our findings corroborate differential underlying regulatory mechanisms of CRP and cytokines and are suggestive of an individual inflammatory marker baseline which is, in part, genetically driven. While prior research has mainly focused on immune marker changes throughout pregnancy, our study suggests that this field could benefit from a focus on intra-individual factors, including metabolic and genetic components.

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Immunologic deviations in recurrent spontaneous abortion mouse model

Cited by 7 publications

References 75 publications

Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

Upregulation of GPR133 expression impaired the phagocytosis of macrophages in recurrent spontaneous miscarriage

Correlation Ratio of Chronic Endometritis With Anamnestic Factors and Ultrasound Characteristics of the Endometrium in Women of Reproductive Age

Inflammatory markers in pregnancy – surprisingly stable. Mapping trajectories and drivers in four large cohorts

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