Immunologic Determination of Seminal Secretions by a Latex Microtiter Technique

Matsuzawa, Shigetaka; Itoh, Yuta; Miyauchi, Chitose; Hara, Mitsuwo; Inoue, Tomoyoshi; Tsuda, Ryouichi

doi:10.1520/jfs12202j

Cited by 17 publications

(3 citation statements)

References 4 publications

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“…The presence of human seminal plasma proteins in biological stains has been demonstrated by an absorption test using anti-human seminal protein rabbit serum. The authors claim this test to be sensitive and highly specific (284). The estimation of time since intercourse has been reported by Rutter et al (285).…”

Section: Forensic Biochemistrymentioning

confidence: 80%

“…An RIA method was described for the detection of human prostrate-specific acid Phosphatase in forensic semen samples in which sperm cels could not be seen microscopially (281). The authors claim this test to be sensitive and highly specific (284). A new method for the detection of l~uman semen by monitoring the electrophoretic pattern of glycylprolyl di eptide aminopeptidase (GP-DAP) has been reported.…”

Section: Trace Evidencementioning

confidence: 99%

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Pharmaceuticals and related drugs

Gilpin¹,

Pachla²,

Ranweiler³

1983

Anal. Chem.

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Forensic Science in Applications Reviews. Given the tremendous diversity of materials received by forensic science facilities and the varied assortment of analytical techniques applied to their characterization, there has, and will remain, serious interest on the part of forensic scientists in the subject content of most reviews appearing within this Journal. It is the aim of this article to augment these reviews with a concise survey of articles appearing in publications that primarily appeal to forensic practitioners. To accomplish this objective, we have focused our attention on the following journals:

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Section: Forensic Biochemistrymentioning

confidence: 80%

Section: Trace Evidencementioning

confidence: 99%

Pharmaceuticals and related drugs

Gilpin¹,

Pachla²,

Ranweiler³

1983

Anal. Chem.

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“…The simplest technique of passive agglutination is the slide method (Watson 1965), in which the formation of coarse agglutinates is investigated with the unaided eye or under weak magnification. A broadly used and sensitive technique is the investigation of agglutinated and precipitated microsphere patterns, which has been performed in most cases with a microtiter plate (Boyden 1951;Stavitsky 1954a; Ransom et al 1968; Matsuzawa and Itoh 1979; Matsuzawa et al 1982), and can offer semi-quantitative results. Recently, erythrocytes as a passive support have been replaced by polystyrene latex or other synthetic polymers, but a disadvantage of these polymers is the time it takes (at least 2 hr) to form precipitation patterns.…”

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confidence: 99%

A Continuously Rotating Passive Agglutination Method Using Rapidly Precipitable Latex Particles and Its Application.

Kimura

Matsuzawa

1991

Tohoku J. Exp. Med.

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A new passive agglutination method is reported.Large, very heavy, uniform latex particles coated with antigens or antibodies were mixed with corresponding antibodies or antigens in reacting wells of an appropriate shape and size. Agglutination results were read after 30 min of continuous rotation at a suitable speed. With this simple and rapid continuously rotating passive agglutination method, we could detect antidinitrophenol monoclonal antibodies, natural anti-dinitrophenol antibodies, rheumatoid factor, allergic antibody to tick, human chorionic gonadotrophin and human IgE. This method is suitable to detect weak and small quantities of antibodies in a brief time.

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Detection of nonprostatic PSA in serum and nonserum samples from women

Filella¹,

Molina²,

Alcover³

et al. 1996

Int. J. Cancer

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Prostate-specific antigen (PSA) has been characterized as a specific prostate marker, although recent studies have suggested the existence of PSA of nonprostatic sources. We have assessed the presence of PSA by an ultrasensitive assay in 276 serum and nonserum samples from women. Our data show the presence of PSA in 90% of nonserum samples, including milk secretions, breast cysts, amniotic fluids and broncho-alveolar washings. We have also detected its presence in 58% of the sera tested, although it only exceeded 0.1 microg/l in 6 of the cases. We conclude that PSA can no longer be considered a specific prostate tissue marker; since the concentrations detected in women's serum are ultralow, this finding appears to interfere minimally with its value as a tumoral marker.

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Immunologic Determination of Seminal Secretions by a Latex Microtiter Technique

Cited by 17 publications

References 4 publications

Pharmaceuticals and related drugs

Pharmaceuticals and related drugs

A Continuously Rotating Passive Agglutination Method Using Rapidly Precipitable Latex Particles and Its Application.

Detection of nonprostatic PSA in serum and nonserum samples from women

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