Immunologic and Osteogeneic Properties of Xenogeneic and Allogeneic Demineralized Bone Transplants

Abstract

Xenografting is increasingly being developed as a response to the shortage of human tissues. However, antigenic components of bone material eliciting immune responses--particularly of cellular nature--are blamed for the reduction of the osteoinductive properties of bone and bone-derived implants. The aim of our study was to compare the immunologic response and osteogenesis induced by antigen-depleted allogeneic and xenogeneic bone-derived implants to that induced by partially antigen-depleted material heterotopically placed (muscular pouch) in rats. Wistar rats received bone-derived implants of different antigeneic condition, from both xenogeneic (rabbit) and allogeneic (rat) origin. After sacrifice, animals were evaluated for osteogenesis and immune response. New bone formation was observed around all bone-derived implants, whether fully or partially antigen-extracted, and from both xenogeneic and allogeneic origin. No significant humoral response resulted following bone implantation. Cellular response showed a similar pattern in partial and fully antigen-extracted bone of both allogeneic and xenogeneic origin. Xenogeneic antigen-extracted bone from safe donor sources could be a suitable solution to human tissue shortage in a near future.

The optimization of pepsin processing time to balance the mechanical and biological properties of xenogeneic bone

“…The xenogeneic bone, as a bone graft material with extensive sources, low cost, matched mechanical properties, and bionic structure, has been applied to repair bone defects since the 17th century 9 . The key point for clinical applications of xenogeneic bone is to eliminate its immunogenic components, leaving only inorganic calcium phosphate components 10 . With the development of processing technologies, many xenogeneic bone products have been explored for clinical application to repair maxillofacial defects 11,12 .…”

Avance de tuberosidad tibial con heteroimplante óseo para el tratamiento de ruptura de ligamento cruzado anterior en caninos

“…Los heteroimplantes óseos procedentes de huesos bovinos procesados (desproteinizado y desantigenizado) se han utilizado experimental y clínicamente demostrando su capacidad osteoinductora (hueso esponjoso) y de resistencia biomecánica (hueso cortical). Estos, además, presentan una ventaja económica (Bojrab 1996;Bojrab y Monnet 2011;Zunino et al 2004).…”

Evaluation of biocompatibility of α-1,3 galactosyltransferase knockout pig bone graft in a rat calvarial defect model

“…특히 신장이나 심장, 간과 같은 다른 연부조직 장기에 비해 골은 면역 반응을 일으키는 단백질이 적으며 동결건조나 탈 회 동결건조, 방사선 조사 등에 의해 골의 특성을 유지하면서 도 간단하게 항원성을 줄일 수 있다고 알려져 왔다 [12][13][14][15].…”