Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice

Xing, Yizhan; Xu, Wei; Yang, Kang; Lian, Xiaohua; Yang, Tian

doi:10.1016/j.acthis.2010.06.006

Cited by 20 publications

(20 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, response to prostaglandin E2 (PGE2) may be modulated by DP-derived Wnt5a in response to Wnt3a from surrounding matrix cells because expression of EP2, a Wnt3a target (25), occurs in DP of anagen hair follicles (26). This speculation is in line with the finding of a study using whisker follicle organ culture, which demonstrated inhibited growth of the hair shaft by Wnt5a (21). In addition, the low expression of Wnt5a in DP in telogen would allow the neogen phase to proceed in response to Wnt3a.…”

Section: Resultssupporting

confidence: 74%

“…In addition, promotion of hair shaft growth by Wnt10b via a canonical Wnt signalling pathway has been reported (17)(18)(19). Wnt5a is a specific DP signature gene (20) and its expression is highest in the anagen phase (21,22). Hu et al (23) recently reported that Wnt5a, as a paracrine factor of DP cells, has a unique function in control of hair follicle differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…Hu et al (23) recently reported that Wnt5a, as a paracrine factor of DP cells, has a unique function in control of hair follicle differentiation. However, in an in vitro study using whisker follicle organ culture, Xing et al (21) reported on inhibition of hair shaft growth by Wnt5a.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Wnt5a attenuates Wnt/β‐catenin signalling in human dermal papilla cells

Kwack

Kim

et al. 2013

Experimental Dermatology

View full text Add to dashboard Cite

Abstract:Findings of recent studies have demonstrated modulation of Wnt/b-catenin signalling by Wnt5a, which is highly expressed in hair follicular dermal papilla (DP) in vivo. Here, we investigated the question of whether Wnt5a can affect canonical Wnt/b-catenin signalling in DP cells. Treatment with Wnt5a resulted in attenuation of Wnt3a-mediated elevation of b-catenin signalling, which was increased by Wnt5a siRNA transfection in cultured DP cells, as examined by reporter assay. In addition, treatment with Wnt5a resulted in repressed Wnt3a-mediated expression of Axin2, EP2 and LEF1 in cultured DP cells, whereas Wnt5a siRNA transfection resulted in increased Wnt3a-mediated expression of the genes in isolated DPs of cultured hair follicles. Moreover, treatment with Wnt5a resulted in attenuation of Wnt3a-mediated accumulation of b-catenin in the nucleus in DP cells. Our data strongly suggest that Wnt5a acts as an autocrine factor and attenuates canonical Wnt signalling pathway in human DP cells.Abbreviations: DPdermal papilla; EP2prostaglandin E receptor 2; LEF1lymphoid enhancer-binding factor 1; rhWnt5arecombinant human Wnt5a; rmWnt3arecombinant mouse Wnt3a.

show abstract

Section: Resultssupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Wnt5a attenuates Wnt/β‐catenin signalling in human dermal papilla cells

Kwack

Kim

et al. 2013

Experimental Dermatology

View full text Add to dashboard Cite

show abstract

“…[18][19][20] Among proteins associated with the Wnt signaling pathway, Wnt5α promotes hair cycle and hair follicle growth. Wnt protein binds to the Wnt receptor, Frizzled (FZ) proteins, and the low-density lipoprotein receptor-related protein (LRP) family and activates the canonical Wnt signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Visible-to-Near IR Quantum Dot–Based Hypermulticolor High-Content Screening of Herbal Medicines for the Efficacy Monitoring of Hair Growth Promotion and Hair Loss Inhibition

Kim

Lim

Lee³

et al. 2013

SLAS Discovery

View full text Add to dashboard Cite

There is a growing interest in alopecia prevention strategies, as the number of alopecia patients is increasing. We examine the efficacy of herbal medicine for hair growth promotion/hair loss inhibition in two cell lines via Western blot and highcontent screening (HCS). Nine herbal extracts were obtained from three different herbal medicine mixtures using 3 different extraction methods. Five target proteins-IGF-1 (insulin-like growth factor-1), TGF-β2 (transforming growth factor-β2), VEGF (vascular endothelial growth factor), DKK-1 (Dickkopf-1), and Wnt5α-were observed for the assessment of hair growth promotion/hair loss inhibition efficacy. The efficacies of nine extracts were compared with minoxidil as control. Efficacy was defined as a rise in the expression levels of IGF-1, VEGF, and Wnt5α but a decrease in DKK-1 and TGF-β2. Intracellular concurrent imaging of these proteins was successfully achieved using HCS, employing visible-to-near infrared probing based on quantum-antibody conjugates and hypermulticolor imaging.

show abstract

“…Therefore, administration of Wnt3a may regulate the expression of BMP-6 which can be beneficial for hair growth6. Recently, it has been reported that Wnt5a is highly expressed by dermal papilla cells during the anagen phase9. It is likely that increased levels of Wnt5a expression in our model may be produced by anagen phase dermal papilla cells.…”

mentioning

confidence: 88%

Effects of Murine Dermal Cells on the Regulation of Hair Growth Is Dependent on the Cell Number and Post-Natal Age of Newborn Mice

Park

Choi

et al. 2012

Ann Dermatol

View full text Add to dashboard Cite

Dermal cells from neonatal mice can initiate the formation of hair follicles (HFs) when combined with adult mouse epidermal cells and transplanted subcutaneously into athymic mice. In the present study, the effects of dermal cells on HF formation were tested in terms of total cell number and the time course of cell harvest. Results demonstrated that the number of dermal cells is critical to the formation of HF. Furthermore, hair forming ability is rapidly decreasing as the neonatal mice age. To examine potential differences in gene expression, cDNA array was performed. Results demonstrate that numerous molecules which are directly involved in receptor and signaling correlated with decreased hair inductivity in early time points after delivery. It is reported that bone morphogenic protein (BMP)-6 and Wnt3a treatment increased hair inductivity of dermal papilla cells. But in our study, no changes were observed in the expression levels of BMP-6 and Wnt3a. However, several Wnt related genes demonstrate increased or decreased expression levels. Thus, our results suggest that co-ordinated regulation of these molecules will be important in hair neogenesis within our model system.

show abstract

Immunolocalization of Wnt5a during the hair cycle and its role in hair shaft growth in mice

Cited by 20 publications

References 27 publications

Wnt5a attenuates Wnt/β‐catenin signalling in human dermal papilla cells

Wnt5a attenuates Wnt/β‐catenin signalling in human dermal papilla cells

Visible-to-Near IR Quantum Dot–Based Hypermulticolor High-Content Screening of Herbal Medicines for the Efficacy Monitoring of Hair Growth Promotion and Hair Loss Inhibition

Effects of Murine Dermal Cells on the Regulation of Hair Growth Is Dependent on the Cell Number and Post-Natal Age of Newborn Mice

Contact Info

Product

Resources

About