Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes

Immunological and molecular diagnosis of cysticercosis

“…69 Yang et al described a 10 kDa antigen from cyst fluid, also belonging to the 8 kDa family, reacting mainly to IgG4 and IgG1. 74 This 10 kDa antigen was also produced in recombinant form and showed a good performance to differentiate active from inactive NCC. 71 Other native antigens under research include parasite proteases.…”

Taenia solium metacestode preparation in rural areas of sub-Saharan Africa: a source for diagnosis and research on cysticercosis

“…Obtained low kDa bands in the immunoblot testing are well known and have been described as diagnostic bands in several previous publications. 22,[24][25][26][27] The single additional band at 14 kDa, which was shown in 6 out of 48 cases, has been also described as diagnostic band before. 27 This study has several limitations: The percentage of positive tested pigs has to be interpreted carefully and cannot be used as prevalence rates: In our study, pigs were tested by convenience sampling in previous known endemic areas.…”

“…Suitability of collected material for immunodiagnosis was proven in a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay by presence of diagnostic banding patterns as previously described, 22,[24][25][26][27] when incubated with confirmed neurocysticercosis serum panels. Obtained low kDa bands in the immunoblot testing are well known and have been described as diagnostic bands in several previous publications.…”

“…Previous reports have indicated a prevalence of porcine cysticercosis in endemic districts between 8.2% and 46.7%. [10][11][12][13] Human and animal disease together cause societal burden and significant economic loss which amounted in the Eastern Cape Province in South Africa to [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] Since T. solium cysticercosis/NCC represents an emerging public health problem in many parts of the world, massive efforts are being made to improve prevention, diagnosis and treatment in humans and swine. 14 For the diagnose of T. solium cysticercosis in humans several serological tests (immunoblot, especially enzyme-linked immunoelectrodiffusion transfer blot [EITB]; antibody-enzyme linked immunosorbent assay [ELISA], antigen-ELISA) are used to confirm clinical and radiological findings.…”

Immunoblot findings of calcareous corpuscles binding proteins in cyst fluid of Taenia solium metacestodes

“…1B). Antigenic protein of 10 kDa is known to be a subunit of a 150 kDa protein in the cyst fluid of T. solium metacestodes and is a highly specific and sensitive antigen for the diagnosis of cysticercosis (Cho et al, 1988(Cho et al, , 1992Yang et al, 1998). This molecule has been cloned and its antigenic sites analyzed (Chung et al, 1999(Chung et al, , 2002.…”