Immunolocalization of progesterone and estrogen receptors in the sperm storage tubules of laying and diethylstilbestrol-injected immature hens

Yoshimura, Y.; Koike, Kazuhiko; Okamoto, Toshikazu

doi:10.1093/ps/79.1.94

Cited by 35 publications

(21 citation statements)

References 21 publications

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“…This hypothesis is supported by studies showing that injections of 17 β-estradiol and progesterone induced SST development in immature turkeys (Pescatore and Marquez,'77) and the presence of estrogen and progesterone receptors in the chicken SST (Yoshimura et al, 2000). However, in turkeys, SST containing spermatozoa have been found in birds with undeveloped ovaries (Bakst,'88,'92), suggesting that baseline levels of these hormones and/or some other factor may be sufficient to induce development of SST.…”

Section: Discussionmentioning

confidence: 73%

Development of sperm storage tubules in the quail during sexual maturation

Holm

Ridderstråle

2001

J. Exp. Zool.

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The development of the utero-vaginal sperm storage tubules (SST) in the quail during sexual maturation was studied using light microscopy and image analysis. SST development starts at around 28 days with low columnar cells, 10.9 +/- 0.7 microm high, found at the base of mucosal folds in the distal uterus. Seven days later, small bud-like invaginations consisting of columnar, 16.4 +/- 1.1 microm high cells with basal nuclei were found in this region. An extremely rapid growth of the oviduct occurred at approximately 40-42 days of age with considerable variation in oviductal length between birds, coefficient of variation (CV) 64.8% and 66.2%, respectively. Two of these birds had SST containing spermatozoa but were not laying. At 49 days, oviductal length was 24 +/- 0.5 cm (CV 2.0%), and all birds had functional SST with spermatozoa and had started to lay. Mature SST consist of columnar, nonciliated cells, 19.8 +/- 0.7 microm high. Although development of SST in the quail, to a large extent, coincides with the development of the rest of the oviduct, the present findings suggest that utero-vaginal sperm storage is possible before the complete maturation of the oviduct and subsequent onset of lay.

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Section: Discussionmentioning

confidence: 73%

Development of sperm storage tubules in the quail during sexual maturation

Holm

Ridderstråle

2001

J. Exp. Zool.

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“…High progesterone may have interfered with sperm release from sperm-storage sites in the infundibulum and/or uterovaginal junction, preventing egg fertilization. Although the mechanism of sperm release is unknown, sperm-storage tubules of laying hens contain progesterone receptors (Yoshimura et al 2000). It is unlikely that only Z-containing eggs were unfertilized but we cannot exclude this possibility.…”

Section: Discussionmentioning

confidence: 93%

High progesterone during avian meiosis biases sex ratios toward females

2005

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Evidence of altered primary sex ratios in birds shows that mothers can manipulate the sex of their offspring before oviposition. In birds, females are the heterogametic sex (ZW) and males are homogametic (ZZ). Sex is determined in the first meiotic division, when one sex chromosome is retained in the oocyte and the other segregates to the polar body. Altered primary sex ratios suggest that birds may be capable of biasing the segregation of sex chromosomes during meiosis I. During the time of meiosis I, follicular steroid production is limited primarily to progesterone (P4). We experimentally manipulated the levels of P4 in female domestic chickens during the approximate time of meiosis I. We advanced the ovulation of the first egg of a sequence (or clutch) with a subcutaneous injection of P4. We found a significant effect of P4 dose on the sex of the resulting egg. The high progesterone group produced 25% males whereas the low progesterone group produced 61% males and the control group produced 63% males in the first ovulation of the sequence. We propose that variation in maternal progesterone during the critical time for genetic sex determination is the mechanism for primary sex ratio manipulation in birds.

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“…To our knowledge, these results provide the first evidence showing that the avian oviduct expresses mPR␣. Before the discovery of the mPR, Yoshimura et al (39) demonstrated the presence of nPR protein in the SST cells by immunohistochemistry in laying chicken, although the function of the nPR remains to be investigated. We also detected both the immunoreactive nPR protein (Fig.…”

Section: Discussionmentioning

confidence: 99%

Progesterone Is a Sperm-Releasing Factor from the Sperm-Storage Tubules in Birds

et al. 2011

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Because of the presence of sperm-storage tubules (SST) in the utero-vaginal junction (UVJ) in the oviduct, once ejaculated sperm have entered the female reproductive tract, they can survive for a prolonged time in domestic birds, although the specific mechanisms involved in the sperm uptake into, maintenance within, and controlled release from the SST remain to be elucidated. In this report, we provide evidence that progesterone triggers the release of the resident sperm from the SST in the UVJ. The ultrastructural observation of the SST indicated that the resident sperm are released from the SST around 20 h after oviposition. When laying birds were injected with progesterone, most of the sperm were released from the SST within 1 h of injection. In situ hybridization analyses demonstrated the presence of the transcripts of membrane progestin receptor α in the UVJ, and the translated proteins were detected in the UVJ extracts by Western blotting. Moreover, the number of secretory granules in the SST epithelial cells fluctuates during the ovulatory cycle, and the progesterone administration mimics this phenomena. A binding assay using [(3)H]-progesterone indicated the presence of a high affinity, limited capacity, saturable and single binding site for [(3)H]-progesterone in the membrane fraction of the UVJ, and this receptor did not interact with the synthetic antiprogestin RU486. These results demonstrated for the first time that the progesterone stimulates the release of the resident sperm from the SST and that the release of the sperm might occur via membrane progestin receptor α-mediating signal transduction.

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Immunolocalization of progesterone and estrogen receptors in the sperm storage tubules of laying and diethylstilbestrol-injected immature hens

Cited by 35 publications

References 21 publications

Development of sperm storage tubules in the quail during sexual maturation

Development of sperm storage tubules in the quail during sexual maturation

High progesterone during avian meiosis biases sex ratios toward females

Progesterone Is a Sperm-Releasing Factor from the Sperm-Storage Tubules in Birds

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