Immunolocalization of Nidogen in Rat Uterus, Oviduct and Ovary

Abstract

This study was designed to determine the distribution patterns of nidogen-1 (N-1) and -2 (N-2) in the rat uterus, oviduct and ovary. For this purpose, 6 female Wistar-albino rats found in proestrous period were used. The tissue samples fixed in 10% neutral formalin were examined immunohistochemically. Sections were incubated with primary goat-polyclonal N-1 and -2 antibody. Both the N-1 and -2 immunoreacted strongly with the uterine luminal and gland epithelium, many cells in the uterine stroma, the oviduct epithelium, the follicular cells of the unilaminar primary follicles, interstitial cells, and atretic follicles. They were moderately expressed in the follicular epithelium of primary and secondary follicles. The immunostaining intensity of N-1 was higher than that of N-2 in theca interna cells of graafian follicles. N-1 and -2 immunostaining were weakly detected in some of the primordial follicles, and in the granulosa lutein cells of corpus luteum. Neither theca externa nor zona pellucida immunoreacted with N-1 and -2.In conclusion, the present study showed that the nidogen are synthesized by the uterine luminal and the endometrial gland epithelium, the oviduct epithelium and ovarian follicles in the rats.

Expression of extracellular matrix proteins nidogen‐1 and legumain in endometrial carcinomas

“…Staining was completed with DAB Chromogen (DBS, Pleasanton, CA, USA) for 1–2 min, and slides were counterstained with Harris's hematoxylin, dehydrated and then cover‐slipped with Permount. All specimens were examined with a light microscope 4,8 …”

“…All specimens were examined with a light microscope. 4,8 Sections were evaluated concerning the localizations of NID1 and LGMN in a semiquantitative manner using a light microscope and selected areas were photographed. The intensity of staining was scored as negative (À), weak (+), moderate (++), or strong (+ ++).…”

“…The intensity of staining was scored as negative (À), weak (+), moderate (++), or strong (+ ++). 4,8 The intensity score was multiplied with the percentage of tumor tissue stained and the resulting score was recorded (range: 0 to 300). We investigated the role of NID1 in tumor invasion by comparing the expression of NID1 in two tumors, superficial and deep.…”

“…Nidogen‐1 serves as a bridge between the ECM molecules, laminin‐1, and type IV collagen. It is included in the composition of basement membranes and it acts as a cell‐adhesion molecule by binding to integrins 4 …”

“…It is included in the composition of basement membranes and it acts as a cell-adhesion molecule by binding to integrins. 4 Elucidation of the steps associated with recurrence, myometrial, ovarian, cervical involvement as well as lymph node metastasis, and positive ascites cytology is crucial. Understanding the mechanisms involved in these steps may represent a critical development for the identification of new therapeutic targets that may predict the spread of cancer.…”