Immunolocalization of natriuretic peptides and their receptors in goat (Capra hircus) heart

Abstract

Natriuretic peptides are structurally similar, but genetically distinct, hormones that participate in cardiovascular homeostasis by regulating blood and extracellular fluid volume and blood pressure. We investigated the distribution of natriuretic peptides and their receptors in goat (Capra hircus) heart tissue using the peroxidase-anti-peroxidase (PAP) immunohistochemical method. Strong staining of atrial natriuretic peptide (ANP) was observed in atrial cardiomyocytes, while strong staining for brain natriuretic peptide (BNP) was observed in ventricular cardiomyocytes. Slightly stronger cytoplasmic C-type natriuretic peptide (CNP) immunostaining was detected in the ventricles compared to the atria. Natriuretic peptide receptor-A (NPR-A) immunoreactivity was more prominent in the atria, while natriuretic peptide receptor-B (NPR-B) immunoreactivity was stronger in the ventricles. Cytoplasmic natriuretic peptide receptor-C (NPR-C) immunoreactivity was observed in both the atria and ventricles, although staining was more prominent in the ventricles. ANP immunoreactivity ranged from weak to strong in endothelial and vascular smooth muscle cells. Endothelial cells exhibited moderate to strong BNP immunoreactivity, while vascular smooth cells displayed weak to strong staining. Endothelial cells exhibited weak to strong cytoplasmic CNP immunoreactivity. Vascular smooth muscle cells were labeled moderately to strongly for CNP. Weak to strong cytoplasmic NPR-A immunoreactivity was found in the endothelial cells and vascular smooth muscle cells stained weakly to moderately for NPR-A. Endothelial and vascular smooth cells exhibited weak to strong cytoplasmic NPR-B immunoreactivity. Moderate to strong NPR-C immunoreactivity was observed in the endothelial and smooth muscle cells. Small gender differences in the immunohistochemical distribution of natriuretic peptides and receptors were observed. Our findings suggest that endothelial cells, vascular smooth cells and cardiomyocytes express both natriuretic peptides and their receptors.

Spatiotemporal expression patterns of natriuretic peptides in rat testis and epididymis during postnatal development

“…Studies have shown that natriuretic peptides (NPs) are involved in the regulation of steroidogenesis in the testis (El‐Gehani, Tena‐Sempere, Ruskoaho, & Huhtaniemi, ). NPs are composed of three structurally closely related but functionally distinct molecules: atrial natriuretic peptide (NPPA), brain natriuretic peptide (NPPB) and C‐type natriuretic peptide (NPPC) (Levin, Gardner, & Samson, ; Öztop, Cinar, & Turk, ). NPs perform their physiological functions by binding to two different membrane‐bound guanylyl cyclase‐coupled receptors (NPR‐A and NPR‐B) and activating cyclic guanosine monophosphate (cGMP) signalling cascades.…”

Seasonal morphology and immunoreactivity of cytokeratin and atrial natriuretic peptide in dromedary camel poll glands

“…ANP and BNP hormones are mainly produced in cardiac muscles (Clavell et al., 1993; Mathew et al., 2017; Minamino et al., 1993; Ruskoaho, 1992; Öztop et al 2018). ANP has also been reported in the respiratory (Tei, 2004) and reproductive (El‐Gehani et al., 2001; Gutkowska et al., 1999; Ivanova et al., 2003; Kim, et al., 1992; Mourdjeva et al., 2001) organs.…”

“…ANP might play an important role in the control of surfactant secreted by alveolar pneumocytes in the fetal lung of sheep (Mathew et al., 2017). In the cardiovascular system ANP and their receptors have also been detected in the vascular endothelial cells, vascular smooth muscles and cardiac muscles of goat (Öztop et al, 2018). ANP and BNP are found to be involved in the regulation of blood pressure and homeostasis of fluid‐volume, depending on their natriuretic and diuretic effect in the kidney and on their relaxation action in vascular smooth muscles (Ruskoaho, 1992).…”

Sperm Functional Genome Associated With Bull Fertility

“…Furthermore, high levels of transmembrane protein 2, prosaposin, and NAD (P) (+)-arginine ADP ribosyltransferase proteins had the highest positive correlations with fertility ranking, whereas quantities of nucleotide exchange factor SIL1, galectin-3-binding protein, and vitamin D-binding protein exhibited the highest negative correlations with fertility ranking ( 77 ) ( Table 2 ). The C-type natriuretic peptide (NPPC) is a member of natriuretic peptides that exerts its physiological functions through binding to two distinct membrane-bound guanylyl cyclases and activating cyclic guanosine monophosphate signaling pathways ( 78 , 79 ). In addition to being synthesized by cardiomyocytes and known to modulate vascular permeability and dilation/constriction, NPPC is also produced locally by Sertoli cells in the testis and serves in an autocrine manner ( 80 ).…”