Immunolocalization of metabotropic glutamate receptor 1α (mGluR1α) in distinct classes of interneuron in the CA1 region of the rat hippocampus

Ferraguti, Francesco; Cobden, Philip M.; Pollard, Marie; Cope, David W.; Shigemoto, Ryuichi; Watanabe, Masahiko; Somogyi, Péter

doi:10.1002/hipo.10163

Cited by 119 publications

(123 citation statements)

References 75 publications

(135 reference statements)

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“…4 B). However, these double-labeled interneurons had dendrites mostly localized to the alveus and adjoining stratum oriens but not to the stratum radiatum in CA1, resembling O-LM cells (Ferraguti et al, 2004).…”

Section: Identification Of Glur4-positive Interneuronsmentioning

confidence: 94%

“…However, these neurons contribute to only a minor subpopulation of the GluR4-immunopositive dendrites we examined because their dendrites are very sparse in stratum radiatum (Ferraguti et al, 2004). The GluR4-immunonegative interneuron dendrites may at least originate from calretinin-and calbindin-containing interneurons (Fig.…”

Section: Identification Of Glur4-immunopositive Interneurons and ⑀2 Amentioning

confidence: 99%

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Target-Cell-Specific Left-Right Asymmetry of NMDA Receptor Content in Schaffer Collateral Synapses in ϵ1/NR2A Knock-Out Mice

Kawakami

Shinohara

et al. 2005

J. Neurosci.

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Input-dependent left-right asymmetry of NMDA receptor ⑀2 (NR2B) subunit allocation was discovered in hippocampal Schaffer collateral (Sch) and commissural fiber pyramidal cell synapses (Kawakami et al., 2003). To investigate whether this asymmetrical ⑀2 allocation is also related to the types of the postsynaptic cells, we compared postembedding immunogold labeling for ⑀2 in left and right Sch synapses on pyramidal cells and interneurons. To facilitate the detection of ⑀2 density difference, we used ⑀1 (NR2A) knock-out (KO) mice, which have a simplified NMDA receptor subunit composition.The labeling density for ⑀2 but not 1 (NR1) and subtype 2/3 glutamate receptor (GluR2/3) in Sch-CA1 pyramidal cell synapses was significantly different between the left and right hippocampus with opposite directions in strata oriens and radiatum; the left to right ratio of ⑀2 labeling density was 1:1.50 in stratum oriens and 1.44:1 in stratum radiatum. No significant difference, however, was detected in CA1 stratum radiatum between the left and right Sch-GluR4-positive (mostly parvalbumin-positive) and Sch-GluR4-negative interneuron synapses. Consistent with the anatomical asymmetry, the amplitude ratio of NMDA EPSCs to non-NMDA EPSCs in pyramidal cells was approximately two times larger in right than left stratum radiatum and vice versa in stratum oriens of ⑀1 KO mice. Moreover, the amplitude of long-term potentiation in the Sch-CA1 synapses of left stratum radiatum was significantly larger than that in the right corresponding synapses. These results indicate that the asymmetry of ⑀2 distribution is target cell specific, resulting in the left-right difference in NMDA receptor content and plasticity in Sch-CA1 pyramidal cell synapses in ⑀1 KO mice.

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Section: Identification Of Glur4-positive Interneuronsmentioning

confidence: 94%

Section: Identification Of Glur4-immunopositive Interneurons and ⑀2 Amentioning

confidence: 99%

Target-Cell-Specific Left-Right Asymmetry of NMDA Receptor Content in Schaffer Collateral Synapses in ϵ1/NR2A Knock-Out Mice

Kawakami

Shinohara

et al. 2005

J. Neurosci.

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Section: Selective Expression Of Mglur8 In Relation To Specific Cell mentioning

confidence: 97%

“…Interneurons receiving mGluR8 innervation and positive for mGluR1a, SOM, and weakly for PV are probably O-LM cells Klausberger et al, 2003;Ferraguti et al, 2004). A few other mGluR8-decorated cells contained CR and may be IS (Freund and Buzsaki, 1996;Ferraguti et al, 2004). The rare calbindin-or CCK-positive neurons innervated by mGluR8 terminals may belong to the Schaffer collateral-associated interneurons expressing both CCK and calbindin (Cope et al, 2002).…”

Section: Selective Expression Of Mglur8 In Relation To Specific Cell mentioning

confidence: 99%

“…Scale bars, 30 m. classes, which include O-LM cells, O-Bi cells, and interneurons selectively targeting other interneurons (IS) (Losonczy et al, 2002;Ferraguti et al, 2004). Because O-LM cells are known to express high levels of postsynaptic mGluR1a receptors (Baude et al, 1993), to contain both SOM and PV (Klausberger et al, 2003;Ferraguti et al, 2004) and also to be heavily decorated by mGluR7a-containing terminals (Shigemoto et al, , 1997, we performed a series of triple-immunolabeling experiments combining antisera for these markers (Table 1). Terminals containing mGluR8a immunoreactivity unevenly innervated mGluR1a-immunolabeled neurons expressing SOM as well as PV (Fig.…”

Section: Mglur1a-expressing Interneurons Are Targeted By Terminals Immentioning

confidence: 99%

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Metabotropic Glutamate Receptor 8-Expressing Nerve Terminals Target Subsets of GABAergic Neurons in the Hippocampus

Ferraguti¹,

Klausberger²,

Cobden³

et al. 2005

J. Neurosci.

121

138

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Presynaptic metabotropic glutamate receptors (mGluRs) show a highly selective expression and subcellular location in nerve terminals modulating neurotransmitter release. We have demonstrated that alternatively spliced variants of mGluR8, mGluR8a and mGluR8b, have an overlapping distribution in the hippocampus, and besides perforant path terminals, they are expressed in the presynaptic active zone of boutons making synapses selectively with several types of GABAergic interneurons, primarily in the stratum oriens. Boutons labeled for mGluR8 formed either type I or type II synapses, and the latter were GABAergic. Some mGluR8-positive boutons also expressed mGluR7 or vasoactive intestinal polypeptide. Interneurons strongly immunopositive for the muscarinic M2 or the mGlu1 receptors were the primary targets of mGluR8-containing terminals in the stratum oriens, but only neurochemically distinct subsets were innervated by mGluR8-enriched terminals. The majority of M2-positive neurons were mGluR8 innervated, but a minority, which expresses somatostatin, was not. Rare neurons coexpressing calretinin and M2 were consistently targeted by mGluR8-positive boutons. In vivo recording and labeling of an mGluR8-decorated and strongly M2-positive interneuron revealed a trilaminar cell with complex spike bursts during theta oscillations and strong discharge during sharp wave/ripple events. The trilaminar cell had a large projection from the CA1 area to the subiculum and a preferential innervation of interneurons in the CA1 area in addition to pyramidal cell somata and dendrites. The postsynaptic interneuron type-specific expression of the high-efficacy presynaptic mGluR8 in both putative glutamatergic and in identified GABAergic terminals predicts a role in adjusting the activity of interneurons depending on the level of network activity.

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Quantitative Assessment of Hippocampus Architecture Using the Optical Disector

Jinno

2013

Neurostereology

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Immunolocalization of metabotropic glutamate receptor 1α (mGluR1α) in distinct classes of interneuron in the CA1 region of the rat hippocampus

Cited by 119 publications

References 75 publications

Target-Cell-Specific Left-Right Asymmetry of NMDA Receptor Content in Schaffer Collateral Synapses in ϵ1/NR2A Knock-Out Mice

Target-Cell-Specific Left-Right Asymmetry of NMDA Receptor Content in Schaffer Collateral Synapses in ϵ1/NR2A Knock-Out Mice

Metabotropic Glutamate Receptor 8-Expressing Nerve Terminals Target Subsets of GABAergic Neurons in the Hippocampus

Quantitative Assessment of Hippocampus Architecture Using the Optical Disector

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