Immunolocalization of melatonin receptors in bovine ovarian follicles and in vitro effects of melatonin on growth, viability and gene expression in secondary follicles

Paulino, Laís R F M; Barroso, P.A.A.; Silva, Bianca.R.; Barroso, Laryssa.G.; Barbalho, Efigênia.C.; Bezerra, F.T.G.; Souza, Ana Liza Paz; Monte, Alane Pains Oliveira do; Silva, Anderson.W.B.; Matos, Maria Helena Tavares de; Silva, José.R.V.

doi:10.1016/j.domaniend.2022.106750

Cited by 12 publications

(7 citation statements)

References 64 publications

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“…Thus, our study confirms that melatonin effectively inhibited ROS generation. Furthermore, the diquat-induced reduction in GPX1 and SOD1 protein abundance, was reversed following treatment of mice with melatonin, a result that is in accordance with previous studies [ 40 , 41 , 42 , 43 ].…”

Section: Discussionsupporting

confidence: 92%

Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice

Yang

Cheng

et al. 2023

Toxics

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Diquat is a fast, potent, and widely used bipyridine herbicide in agriculture and it induces oxidative stress in several animal models. However, its genotoxic effects on the male reproductive system remain unclear. Melatonin is an effective free-radical scavenger, which has antioxidant and anti-apoptotic properties and can protect the testes against oxidative damage. This study aimed to investigate the therapeutic effects of melatonin on diquat-induced testicular injury in mice. The results showed melatonin treatment alleviated diquat-induced testicular injury, including inhibited spermatogenesis, increased sperm malformations, declined testosterone level and decreased fertility. Specifically, melatonin therapy countered diquat-induced oxidative stress by increasing production of the antioxidant enzymes GPX1 and SOD1. Melatonin treatment also attenuated diquat-induced spermatogonia apoptosis in vivo and in vitro by modulating the expression of apoptosis-related proteins, including P53, Cleaved-Caspase3, and Bax/Bcl2. Moreover, melatonin restored the blood-testicular barrier by promoting the expression of Sertoli cell junction proteins and maintaining the ordered distribution of ZO-1. These findings indicate that melatonin protects the testes against diquat-induced damage by reducing oxidative stress, inhibiting apoptosis, and maintaining the integrity of the blood–testis barrier in mice. This study provides a theoretical basis for further research to protect male reproductive health from agricultural pesticides.

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Section: Discussionsupporting

confidence: 92%

Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice

Yang

Cheng

et al. 2023

Toxics

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“…The presence of melatonin in culture medium did not influence expression of SOD, CAT, PRDX-6 or GPXI mRNA oocyte-granulosa complexes from early antral follicles cultured for 8 days. However, Paulino et al (2022) reported that 10 −7 M melatonin increased mRNA expression for CAT and SOD enzymes in bovine secondary follicles cultured in vitro for 18 days. These can be due to differences in culture periods and the fact that we evaluated mRNA expression in oocyte-granulosa complexes instead of whole follicles.…”

Section: Discussionmentioning

confidence: 97%

“…However, Paulino et al. (2022) reported that 10 −7 M melatonin increased mRNA expression for CAT and SOD enzymes in bovine secondary follicles cultured in vitro for 18 days. These can be due to differences in culture periods and the fact that we evaluated mRNA expression in oocyte‐granulosa complexes instead of whole follicles.…”

Section: Discussionmentioning

confidence: 99%

“…As an alternative to improve follicular ultrastructure and to control oxidative stress during in vitro culture of bovine early antral follicles, melatonin has been highlighted due to the its protective effect on murine preantral follicles (Jang et al., 2016; Kandemir et al., 2017), and the ability to improve the development of secondary follicles, and oocyte competence in pigs (Cao et al., 2019). In addition, melatonin promotes activation of primordial follicles during in vitro culture of bovine ovarian cortical tissue (Cavalcante et al., 2019), and growth of in vitro cultured secondary follicles (Paulino et al., 2022). Therefore, we formulate the hypothesis that melatonin is able to improve viability, ultrastructural integrity and mitochondrial activity, as well as to preserve chromatin and to regulate the ROS levels and expression of mRNA for antioxidant enzymes of oocyte‐granulosa complexes (OGC) of in vitro cultured early antral follicles.…”

Section: Introductionmentioning

confidence: 99%

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Melatonin improves the viability and ultrastructure of bovine oocyte‐granulosa complexes of in vitro cultured early antral follicles

Silva,

Nascimento,

Costa

et al. 2024

Reprod Domestic Animals

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This study aims to investigate the effects of melatonin on follicular growth, viability and ultrastructure, as well as on the levels of mRNA for antioxidant enzymes, reactive oxygen species (ROS) and meiotic progression in oocytes from in vitro cultured bovine early antral follicles. To this end, isolated early antral follicles (500–600 μm) were cultured in TCM‐199+ alone or supplemented with 10−6, 10−7 or 10−8 M melatonin at 38.5°C with 5% CO2 for 8 days. Follicle diameters were evaluated at days 0, 4 and 8 of culture. At the end of culture, ultrastructure, chromatin configuration, viability (calcein‐AM and ethidium homodimer‐1 staining), and the levels of ROS and mRNA for catalase (CAT), superoxide dismutase (SOD) and peroxiredoxin 6 (PRDX6) and glutathione peroxidase (GPx) were investigated in oocyte‐granulosa cell complexes (OGCs). The results showed that early antral follicles cultured with 10−6 and 10−8 M melatonin had a progressive and significant increase in their diameters throughout the culture period (p < .05). Additionally, oocytes from follicles cultured with 10−7 or 10−8 M melatonin had increased fluorescence for calcein‐AM, while those cultured with 10−6 or 10−7 M had reduced fluorescence for ethidium homodimer‐1. Different from follicles cultured in other treatments, those cultured with 10−8 M melatonin had well‐preserved ultrastructure of oocyte and granulosa cells. Melatonin, however, did not influence the levels of ROS, the mitochondrial activity, oocyte meiotic resumption and expression mRNA for SOD, CAT, GPX1 and PRDX6. In conclusion, the presence of 10−8 M melatonin in culture medium improves viability and preserves the ultrastructure of oocyte and granulosa cells of early antral follicles cultured in vitro.

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“…In addition, the classic membrane receptors for melatonin, MT1 and MT2, are found in several areas of the ovary, e.g., oocyte, granulosa cells, luteal cells, etc. [ 48 , 49 , 50 , 51 ].…”

Section: Melatonin: Redox Mechanisms For Preserving Reproductive Healthmentioning

confidence: 99%

Aging-Related Ovarian Failure and Infertility: Melatonin to the Rescue

et al. 2023

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Aging has a major detrimental effect on the optimal function of the ovary with changes in this organ preceding the age-related deterioration in other tissues, with the middle-aged shutdown leading to infertility. Reduced fertility and consequent inability to conceive by women in present-day societies who choose to have children later in life leads to increased frustration. Melatonin is known to have anti-aging properties related to its antioxidant and anti-inflammatory actions. Its higher follicular fluid levels relative to blood concentrations and its likely synthesis in the oocyte, granulosa, and luteal cells suggest that it is optimally positioned to interfere with age-associated deterioration of the ovary. Additionally, the end of the female reproductive span coincides with a significant reduction in endogenous melatonin levels. Thus, the aims are to review the literature indicating melatonin production in mitochondria of oocytes, granulosa cells, and luteal cells, identify the multiple processes underlying changes in the ovary, especially late in the cessation of the reproductive life span, summarize the physiological and molecular actions of melatonin in the maintenance of normal ovaries and in the aging ovaries, and integrate the acquired information into an explanation for considering melatonin in the treatment of age-related infertility. Use of supplemental melatonin may help preserve fertility later in life and alleviate frustration in women delaying childbearing age, reduce the necessity of in vitro fertilization–embryo transfer (IVF-ET) procedures, and help solve the progressively increasing problem of non-aging-related infertility in women throughout their reproductive life span. While additional research is needed to fully understand the effects of melatonin supplementation on potentially enhancing fertility, studies published to date suggest it may be a promising option for those struggling with infertility.

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Immunolocalization of melatonin receptors in bovine ovarian follicles and in vitro effects of melatonin on growth, viability and gene expression in secondary follicles

Cited by 12 publications

References 64 publications

Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice

Melatonin Ameliorates Diquat-Induced Testicular Toxicity via Reducing Oxidative Stress, Inhibiting Apoptosis, and Maintaining the Integrity of Blood-Testis Barrier in Mice

Melatonin improves the viability and ultrastructure of bovine oocyte‐granulosa complexes of in vitro cultured early antral follicles

Aging-Related Ovarian Failure and Infertility: Melatonin to the Rescue

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