Immunolocalization of insulin, glucagon, pancreatic polypeptide, and somatostatin in the pancreatic islets of the possum, Trichosurus vulpecula

Reddy, Shiva; Bibby, N.J.; Fisher, Sophie; Rb, Elliott

doi:10.1016/0016-6480(86)90042-0

Cited by 12 publications

(10 citation statements)

References 16 publications

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“…They were also demonstrated associated with acinar cells and pancreatic duct. However, unlike other researchers, Reddy et al (1986) reported that these-immunoreactive cells were observed in most islets where they occurred as groups of cells peripherally and within the pancreatic islets of several marsupial species. In the present study, most of insulin-immunoreactive cells were restricted to the central regions of islets similar to those of previous rodents (Sasaki et al, 1991;Warbritton et al, 1994;Gomez-Dumm et al, 1995;Wieczorek et al, 1998;Yukawa et al, 1999;Camihort et al, 2000).…”

Section: Discussioncontrasting

confidence: 44%

An immunohistochemical study of pancreatic endocrine cells in SKH-1 hairless mice

Hs²,

Jh³

2010

Eur J Histochem

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SUMMARYThe regional distribution and frequency of the pancreatic endocrine cells in the SKH-1 hairless mouse were studied by an immunohistochemical (peroxidase anti-peroxidase; PAP) method using four types of specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (PP). The pancreas of mice were divided into three portions; pancreatic islets, exocrine and pancreatic ducts. The pancreatic islets were further subdivided into three regions (central, mantle and peripheral region) according to their located types of immunoreactive cells. In the pancreatic islet portions, insulin-immunoreactive cells were located in the central and mantle regions with 84.60 ± 7.65 and 33.00 ± 12.45/100 cells frequencies, respectively, but most of somatostatin-, glucagon-and PPimmunoreactive cells were detected in the mantle and peripheral regions. In the mantle region, somatostatin-, glucagon-and PP-immunoreactive cells were demonstrated with 28.70 ± 9.91, 52.00 ± 14.05 and 2.60 ± 1.51/100 cells frequencies, respectively, and showed 6.20 ± 2.86, 15.30 ± 5.31 and 21.50 ± 10.28/100 cells frequencies, respectively in peripheral regions. However, glucagon-immunoreactive 229 cells were also demonstrated in the central regions with 4.00 ± 2.83/100 cells frequency. In the exocrine portions, insulin-, glucagon-, somatostatin-and PPimmunoreactive cells were demonstrated in the SKH-1 mouse with 0.90 ± 0.74, 0.80 ± 0.79, 4.90 ± 3.54 and 2.70 ± 1.34/100 cells frequencies, respectively. In the pancreatic duct portions, insulin-, glucagon-and somatostatin-immunoreactive cells were demonstrated in the subepithelial connective tissues and showed islet-like appearances with 30.30 ± 14.67, 2.70 ± 3.13 and 5.90 ± 4.23/100 cells frequencies, respectively. However, no PP-immunoreactive cells were demonstrated in these regions. In conclusion, some peculiar distributional patterns of pancreatic endocrine cells were found in the SKH-1 hairless mouse.

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Section: Discussioncontrasting

confidence: 44%

An immunohistochemical study of pancreatic endocrine cells in SKH-1 hairless mice

Hs²,

Jh³

2010

Eur J Histochem

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“…In the kangaroo species examined, the β-cells are found in peripheral clusters, with occasional β-cells in the islet interior which is composed of α-cells 17. In Trichosurus vulpecula , β-cells are found in the periphery and α-cells in the islet center, except where they reached the periphery through β-cell clusters 17,19. δ-cells are reportedly found throughout the islet 17.…”

Section: Marsupialmentioning

confidence: 93%

“…The α-cells are the predominant endocrine cells comprising up to 70% of the islet cells, depending on the species 17. A predominance of α-cells has also been reported in opossum species, including the Virginia opossum ( Didelphis virginiana ) and the common brushtail opossum ( Trichosurus vulpecula ; β-cells and α-cells, ~50% and ~40% of endocrine cells, respectively) 17–19…”

Section: Marsupialmentioning

confidence: 99%

“…Differences in islet structure have additionally been noted in studies of more distantly related organisms as well. The expanded presence of α-cells in marsupial islets17–19 and δ-cells in bird islets,16 and the presence of scattered endocrine cells throughout the pancreatic tissue and absence of distinct islets in some reptiles20 are only a few such examples.…”

Section: Introductionmentioning

confidence: 99%

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Pancreatic islet plasticity: Interspecies comparison of islet architecture and composition

Steiner¹,

Kim²,

Miller³

et al. 2010

Islets

397

339

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The pancreatic islet displays diverse patterns of endocrine cell arrangement. The prototypic islet, with insulin-secreting β-cells forming the core surrounded by other endocrine cells in the periphery, is largely based on studies of normal rodent islets. Recent reports on large animals, including humans, show a difference in islet architecture, in which the endocrine cells are randomly distributed throughout the islet. This particular species difference has raised concerns regarding the interpretation of data based on rodent studies to humans. On the other hand, further variations have been reported in marsupials and some nonhuman primates, which possess an inverted ratio of β-cells to other endocrine cells. This review discusses the striking plasticity of islet architecture and cellular composition among various species including changes in response to metabolic states within a single species. We propose that this plasticity reflects evolutionary acquired adaptation induced by altered physiological conditions, rather than inherent disparities between species.

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“…In the possum, Trichosurus vulpecula, it was described by another group that insulin cells were found in islets not only centrally but also in the periphery of islets, and in some islets the glucagon cells were the dominant cell type, found both centrally and in the mantle (Reddy et al 1986). PP cells were quite rare with usually only one or two per islet, while somatostatin cells were mainly in the periphery.…”

Section: Marsupialsmentioning

confidence: 97%

The Comparative Anatomy of Islets

Heller¹

2014

Islets of Langerhans

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Immunolocalization of insulin, glucagon, pancreatic polypeptide, and somatostatin in the pancreatic islets of the possum, Trichosurus vulpecula

Cited by 12 publications

References 16 publications

An immunohistochemical study of pancreatic endocrine cells in SKH-1 hairless mice

An immunohistochemical study of pancreatic endocrine cells in SKH-1 hairless mice

Pancreatic islet plasticity: Interspecies comparison of islet architecture and composition

The Comparative Anatomy of Islets

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